Agilent Technologies Inc. has introduced Agilent SurePrint G3 Gene Expression Microarrays, providing up to one million features per standard 1-by-3-inch slide for added throughput, unique coverage which includes both coding and non-coding RNA.
These third-generation microarrays maintain Agilent’s traditional high level of sensitivity to detect low-expressing genes, along with a high level of accuracy. High-quality data is generated using 60-mer probes fabricated by the latest Agilent SurePrint technology.
“The G3 format provides more coverage based on up-to-date content while also incorporating new non-coding RNA content,” said Chris Grimley, Agilent senior marketing director, Genomics. “In addition, users can run eight whole-genome experiments per slide, increasing throughput and reducing costs for microarrays, reagents and labor.”
For the first time on a commercial product, catalog Agilent G3 Gene Expression Microarrays combine coding and non-coding RNAs on a single array. This comprehensive content includes lincRNA (long intergenic non-coding RNAs) based on novel content from a collaboration with the John Rinn Laboratory at the Broad Institute of MIT and Harvard. The higher density of the Agilent SurePrint G3 Gene Expression Microarrays accommodates the lincRNA content, along with mRNA probes.
“We have been running arrays for years but now we can include the missing lincRNAs,” said Rinn. “Having lincRNAs and protein coding genes on the same arrays provides synergistic data content that allows us to predict what large non-coding RNAs may be doing by correlation with protein coding genes.”
Agilent G3 Gene Expression Microarrays are available in catalog and custom configurations. Customers use Agilent’s exclusive online eArray tool to design custom arrays. Custom configurations offer virtually unlimited options, permitting from 60,000 probes on each of eight arrays per slide to one million probes on a single array. Users can, for example, combine human and pathogen probes while realizing the throughput and low cost of multiplexing experiments.
When used with the new Low Input Quick Amp Labeling Kit, sample input for the G3 Gene Expression Microarray is as low as 10 nanograms for the 8-by-60K format, preserving precious sample while providing cost per experiment.