Dolomite has successfully developed an innovative Droplet System for the encapsulation of single cells or beads into droplets which benefits a wide range of applications including single cell analysis, high throughput screening and droplet PCR.
The system represents a major breakthrough in microfluidics by providing users with the tools required to perform assays on individual cells in picolitre volume environments.
This novel technology was demonstrated by feeding 2.8$m diameter magnetic beads into monodisperse 100$m diameter droplets at a ratio of 1 bead per droplet with minimal bead losses.
Featuring Dolomite’s standard microfluidic components, the new system allows users to load single cells or beads into droplets.
The Mitos P-Pump allows stirring of the input fluid reservoir, to provide an even distribution of beads and delivers a pulseless and stable flow for excellent control of droplet volumes.
Reliable and leak-free connections are made from pump to tube to chip using Dolomite’s Multiflux® Linear Connectors.
Droplets are isolated in an inert fluorocarbon carrier stream to prevent cross contamination and are stabilized with Pico-Surf™, a biocompatible surfactant designed specifically for generating and stabilizing pico-droplets.