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Dolomite Develops Novel Droplet System

Published: Monday, May 21, 2012
Last Updated: Monday, May 21, 2012
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New system for the encapsulation of single cells or beads into droplets.

Dolomite has successfully developed an innovative Droplet System for the encapsulation of single cells or beads into droplets which benefits a wide range of applications including single cell analysis, high throughput screening and droplet PCR.

The system represents a major breakthrough in microfluidics by providing users with the tools required to perform assays on individual cells in picolitre volume environments.

This novel technology was demonstrated by feeding 2.8$m diameter magnetic beads into monodisperse 100$m diameter droplets at a ratio of 1 bead per droplet with minimal bead losses.

Featuring Dolomite’s standard microfluidic components, the new system allows users to load single cells or beads into droplets.

The Mitos P-Pump allows stirring of the input fluid reservoir, to provide an even distribution of beads and delivers a pulseless and stable flow for excellent control of droplet volumes.

Reliable and leak-free connections are made from pump to tube to chip using Dolomite’s Multiflux® Linear Connectors.

Droplets are isolated in an inert fluorocarbon carrier stream to prevent cross contamination and are stabilized with Pico-Surf™, a biocompatible surfactant designed specifically for generating and stabilizing pico-droplets.


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