Corporate Banner
Satellite Banner
Automation & Microfluidics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Bio-Rad Introduces First ProteOn™ Sensor Chip

Published: Thursday, May 24, 2012
Last Updated: Thursday, May 24, 2012
Bookmark and Share
Product capable of label-free interaction analysis of membrane proteins with peptides and small molecules.

Drug Discovery Researchers Get Powerful Tool for Screening Membrane Targets.

Bio-Rad Laboratories, Inc. announced the launch of two surface plasmon resonance (SPR) kits that, when used with the ProteOn XPR36 protein interaction analysis system, enable for the first time the accurate analysis of lipids and membrane proteins with peptides and small molecules.

G-coupled protein receptors, 7-transmembrane receptors, and other integral membrane proteins constitute more than 40% of new drug targets. The new kits — the novel hydrophilic ProteOn LCP liposome capturing kit and the lipophilic ProteOn GLC lipid kit — stably and selectively capture lipids and lipid assemblies for easier screening and analysis of membrane protein targets during drug discovery.

ProteOn_LCP_Capturning_Reag.gif

ProteOn LCP Liposome Capturing Kit

The ProteOn LCP sensor employs memLayer technology to selectively and stably capture lipid assemblies. The “native-lipid like” environment of the chip surface where proteins are captured ensures high quality data.

"The memLayer approach offers an interesting alternative,” said Maria Pavlaki, a senior researcher at Democritus University of Thrace in Greece. “The proteins can be in a more native-like environment (lipoparticles or liposomes) that resembles the cell membrane and are not ‘squashed’ on the surface.”

Capable of capturing multiple layers of lipid assemblies, the ProteOn LCP liposome capturing kit SPR kit is the first to allow label-free detection for binding analysis of peptides and small molecules. And, it empowers researchers to gauge drug absorption by monitoring interactions at the lipid’s surface and tracking uptake of molecules into the assembly.

The ProteOn LCP capturing reagent kit, included in the ProteOn LCP liposome capturing kit, is used to activate the LCP sensor chip with a biotinylated DNA tag that then hybridizes to DNA-tagged lipid assemblies containing membrane proteins of interest.

The surface is easily regenerated with water and ready for re-use.

ProteOn GLC Lipid Kit

The ProteOn GLC lipid kit provides researchers with a basic, easy-to-use option to study interactions of membranes with proteins and peptides. Biopharmaceutical researchers use the kit to capture lipoparticles containing membrane protein drug targets of interest. Antibodies against those drug targets are then screened to measure binding kinetics, an important step in biotherapeutic development.

Researchers can customize the lipophilicity of the GLC sensor surface before capturing lipid assemblies (e.g. proteoliposomes and lipoparticles) by controlling the amount of alkylamine that is bound to the sensor surface. This provides greater experimental flexibility and, because the surface is stable and regenerable, researchers have greater confidence in the quality of data collected over the course of a screening protocol.

"I am very happy with the GLC lipid kit,” said Fang Yi, Ph.D., a scientist at Janssen Biotech “It’s rather straightforward, with very stable capture, high capacity, reproducibility, and also low non-specific binding. I would definitely like to use it for my future lipoparticle characterization studies.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Swiss Study Describes new Human Cytokine Research
Bio-Rad’s technical note describes a human cytokine study undertaken by Swiss researchers who used its Bio-Plex® suspension array system.
Tuesday, December 09, 2008
Bio-Rad Receives a new U.S. Patent for its BioOdyssey Calligrapher Miniarrayer
The patent enables the BioOdyssey Calligrapher MiniArrayer system to maintain movements that are required for microarrying in a benchtop instrument.
Friday, January 18, 2008
Testing Results of the BioOdyssey Calligrapher MiniArrayer for Clinical Research Available
Bio-Rad announces the availability of a technical bulletin describing results of the testing of the BioOdyssey™ Calligrapher™ MiniArrayer.
Thursday, November 08, 2007
Scientific News
Releasing Cancer Cells for Better Analysis
A new device developed at the University of Michigan could provide a non-invasive way to monitor the progress of an advanced cancer treatment.
Releasing Cancer Cells for Better Analysis
A new device developed at the University of Michigan could provide a non-invasive way to monitor the progress of an advanced cancer treatment.
Lab-on-a-Chip for Detecting Glucose
By integrating microfluidic chips with fiber optic biosensors, researchers in China are creating ultrasensitive lab-on-a-chip devices to detect glucose levels.
Soy Shows Promise as Natural Anti-Microbial Agent
Soy isoflavones and peptides may inhibit the growth of microbial pathogens that cause food-borne illnesses, according to a new study from University of Guelph researchers.
Soy Shows Promise as Natural Anti-Microbial Agent
Researchers from University of Guelph show that soy isoflavones and peptides could be used to reduce microbial contamination of food.
Parsortix Demonstrates Benefits Over Marker-Based Systems
Research published online in the International Journal of Cancer, shows the ParsortixTM System efficiently captures and harvests intact, viable circulating tumour cells (CTCs), including EpCAM-negative CTCs, to allow for broader downstream CTC analysis.
Experimental Therapy For Brain Cancer Could Prevent Drug Resistance
Information from penny-sized microfluidic chips allowed researchers to anticipate resistance to cancer treatment.
3D Printing of Lego Fluidics
Study shows how 3D printing can open up microfluidic technology to a wider audience.
New Method to Preserve Device to Monitor HIV Treatment
Inspired by pregnancy tests, scientists have developed a method to store microfluidic devices for months without refrigeration, giving developing countries an inexpensive and reliable way to treat patients.
Migration Creates Cancer Cell Vulnerabilities
Scientists found that migration can damage cancer cells’ nuclei and DNA, requiring repairs for their survival. The results may open new avenues for targeting metastatic cancer.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!