Corporate Banner
Satellite Banner
Automation & Microfluidics
Scientific Community
Become a Member | Sign in
Home>News>This Article

Density-Dependent Separation of Encapsulated Cells in a Microfluidic Channel

Published: Wednesday, June 13, 2012
Last Updated: Wednesday, June 13, 2012
Bookmark and Share
Cell encapsulation is a highly promising method for forming spherical cell bodies, which can mimic a multi-cellular in-vivo environment, for use as a three-dimensional cell culture model.

This study presents a method for density-based separation of monodisperse encapsulated cells using a standing surface acoustic wave (SSAW) in a microchannel. Even though monodisperse polymer beads can be generated by the state-of-the-art technology in microfluidics, the quantity of encapsulated cells cannot be controlled precisely. In the present study, mono-disperse alginate beads in a laminar flow can be separated based on their density using acoustophoresis. A mixture of beads of equal sizes but dissimilar densities was hydrodynamically focused at the entrance and then actively driven toward the sidewalls by a SSAW. The lateral displacement of a bead is proportional to the density of the bead, i.e., the number of encapsulated cells in an alginate bead. Under optimized conditions, the recovery rate of a target bead group (large-cell-quantity alginate beads) reached up to 97% at a rate of 2300 beads per minute. A cell viability test also confirmed that the encapsulated cells were hardly damaged by the acoustic force. Moreover, cell-encapsulating beads that were cultured for 1 day were separated in a similar manner. In conclusion, this study demonstrated that a SSAW can successfully separate monodisperse particles by their density. With the present technique for separating cell-encapsulating beads, the current cell engineering technology can be significantly advanced.

This article was published online in Biomicrofluidics and is free to access.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Integration of Enabling Methods for Automated Flow Preparation of Piperazine-2-Carboxamide
This work describes the application of automation to performing routine research tasks and how the application of remote monitoring can improve safety and efficiency.
Monday, May 19, 2014
All-Aqueous Multiphase Microfluidics
This paper reviews recent progress in the generation of all-aqueous emulsions and jets in microfluidic systems.
Thursday, February 06, 2014
Rapid Sub-attomole MicroRNA Detection on a Portable Microfluidic Chip
Researchers describe two key technologies to solve one of the major issues for point of care testing application.
Thursday, February 06, 2014
Microfluidics for Synthesis of Peptide-Based PET Tracers
Microfluidics is a very promising technology to meet the increased demand for peptide-based PET tracers.
Thursday, January 09, 2014
Microfluidic Separation of Live and Dead Yeast Cells using Reservoir-Based Dielectrophoresis
Cell separation is an essential step in biological research and has important applications in many areas such as environmental monitoring, food production, and pharmaceutical industry.
Friday, September 06, 2013
The Future of Microfluidics for Biomarker Detection
This article outlines the present status of microfluidics for biomarker detection.
Friday, September 06, 2013
Recent Progress in Lab-on-a-Chip Technology and Its Potential Application to Clinical Diagnoses
This study presents the construction of the lab-on-a-chip (LOC) system for the miniaturization of conventional laboratory apparatuses.
Friday, June 21, 2013
Electron Beam Fabrication of a Microfluidic Device for Studying Submicron-Scale Bacteria
This study presents an EBL and poly(dimethylsiloxane) (PDMS) [28] soft-lithography [29] protocol for the fabrication of a micro?uidic device for microbial studies.
Friday, April 26, 2013
Simple Filter Microchip for Rapid Separation of Plasma and Viruses from Whole Blood
Scientists have developed a disposable, pump-free, size exclusion–based filter microchip that can be used for plasma and virus separation from unprocessed whole blood samples in resource-constrained settings.
Wednesday, October 17, 2012
Microfluidics Based Phantoms of Superficial Vascular Network
This paper presents a method for fast prototyping of phantoms simulating a superficial vasculature network, based on microfluidics.
Wednesday, July 11, 2012
Sheathless Size-based Acoustic Particle Separation
The separation of particles or cells is critical for many biological and biomedical applications, including cell biology, diagnostics and therapeutics. This paper presents a novel sheathless SSAW based particle separation technique in a microfluidic channel.
Wednesday, May 16, 2012
Fluorescence Detection Methods for Microfluidic Droplet Platforms
This article reports the entire experimental process for detection of single cells and the mapping of mixing processes inside picoliter-volume droplets, including microfluidic chip fabrication, the optical setup and the process of droplet generation and detection.
Wednesday, May 16, 2012
Lipid-based Passivation in Nanofluidics
This study demonstrates the use of lipid bilayers as a passivation layer in nanofluidic networks, consisting of nanochannels and nanoslits, fabricated in fused silica.
Wednesday, May 16, 2012
Microfluidic Chip for Molecular Amplification of Influenza A RNA in Human Respiratory Specimens
This paper demonstrates a proof-of-concept microfluidic chip using clinical specimens collected over a two-year period from patients with suspected influenza.
Wednesday, April 11, 2012
Single-Cell Chemical Lysis on Microfluidic Chips with Arrays of Microwells
This study looks at the looks at a way of accurately analysing individual cells in order to investigate cellular content.
Thursday, March 08, 2012
Scientific News
Chip-Based Technology Enables Reliable Direct Detection of Ebola Virus
Hybrid device integrates a microfluidic chip for sample preparation and an optofluidic chip for optical detection of individual molecules of viral RNA.
Stem Cell Research Hints at Evolution of Human Brain
Researchers at UC San Francisco have succeeded in mapping the genetic signature of a unique group of stem cells in the human brain that seem to generate most of the neurons in our massive cerebral cortex.
Developing a Breathalyzer-Type Low Blood Sugar Warning Device For Diabetes
A multidisciplinary team of researchers at Indiana University-Purdue University Indianapolis has been awarded a $738,000 National Science Foundation grant to develop a breathalyzer-type device to detect the onset of hypoglycemia, or low blood sugar episodes, in people with diabetes.
Smartphone App to Monitor Serious Blood Disorder
A researcher from Florida Atlantic University has come up with a unique way to monitor sickle cell disease -- a serious blood disorder -- using a smart phone.
Preventing Crystallization to Improve Drug Efficiency
Esther Amstad and an international team of researchers have developed a method to increase the solubility of poorly soluble substances, such as many of the newly developed drugs.
‘Lab-on-a-Chip’ Technology Cuts Costs of Lab Tests
With ability to analyze minuscule amounts of fluid, Rutgers breakthrough could also promote central nervous system and joint research.
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
Capturing Cell Growth in 3-D
Spinout’s microfluidics device better models how cancer and other cells interact in the body.
Device May Detect Urinary Tract Infections Faster
A Lab-on-a-Disc platform developed by a German and Irish team of researchers dramatically cut the time to detect bacterial species that cause urinary tract infections -- a major cause of sepsis.
Automation Abound at AACC in Atlanta
Discover the latest breakthroughs, trends and products from the AACC Annual Meeting & Clinical Lab Expo.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos