Corporate Banner
Satellite Banner
Automation & Microfluidics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Portable Device Enables RNA Detection from Ultra-Small Sample

Published: Friday, November 09, 2012
Last Updated: Friday, November 09, 2012
Bookmark and Share
A new power-free microfluidic chip developed by researchers at the RIKEN Advanced Science Institute (ASI) enables detection of microRNA from extremely small sample volume in only 20 minutes.

By drastically reducing the time and quantity of sample required for detection, the chip lays the groundwork for early-stage point-of-care diagnosis of diseases such as cancer and Alzheimer's.

MicroRNAs (miRNAs) are small, non-coding RNA molecules that regulate gene expression in a wide range of biological processes including development, cell proliferation, differentiation and cell death (apoptosis). Concentration of certain miRNA in body fluids increases with the progression of diseases such as cancer and Alzheimer's, generating hope that these short RNA may hold the key to faster, more accurate diagnosis. Currently available techniques for sensitive miRNA detection, however, require days to reach a diagnosis and involve equipment operated only by trained personnel, making them impractical for use in many situations.

The research team set out to overcome these obstacles by developing a device that enables fast, easy-to-use point-of-care (POC) diagnosis from only a very small sample. In earlier research, the team developed a device in the form of a microchip which uses polydimethylsiloxane (PDMS), a silicone compound known for its air absorption properties, to pull reagents into a capture probe for analysis. This pumping technique simplified design by eliminating the need for external power sources, but the device required a quantity of sample too large for practical applications.

The new device also uses PDMS as an air pump, but drastically improves the method's sensitivity through a signal amplification method called laminar flow-assisted dendritic amplification (LFDA). First, DNA fragments which bond to specific miRNA sequences are fixed to a glass surface along with the miRNA sample to be analyzed, and then sandwiched under a layer of PDMS with channels in it (Figure 1). Emptied of air in a vacuum, the PDMS layer induces a pump effect which pulls amplification reagents, inserted at the channel inlets, into the channels and into contact with the miRNA, creating fluorescence-labeled dendritic structures that grow over time and can be quickly detected.

The sensitivity of this technique drastically reduces the sample quantity required for diagnosis to only 0.25 attomoles (10-18 mole), a thousand-fold improvement over the team's earlier model. Together with its detection time of only 20 minutes, these properties make the self-powered device ideal for use in resource-poor environments, promising portable point-of-care diagnosis for millions in developing countries and around the world.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
Capturing Cell Growth in 3-D
Spinout’s microfluidics device better models how cancer and other cells interact in the body.
Device May Detect Urinary Tract Infections Faster
A Lab-on-a-Disc platform developed by a German and Irish team of researchers dramatically cut the time to detect bacterial species that cause urinary tract infections -- a major cause of sepsis.
Automation Abound at AACC in Atlanta
Discover the latest breakthroughs, trends and products from the AACC Annual Meeting & Clinical Lab Expo.
Real-Time Data for Cancer Therapy
Biochemical sensor implanted at initial biopsy could allow doctors to better monitor and adjust cancer treatments.
Lab-on-a-Chip Offers Promise for TB and Asthma Patients
A device to mix liquids using ultrasonics is the first and most difficult component in a miniaturized system for low-cost analysis of sputum from patients with pulmonary diseases such as tuberculosis and asthma.
Paving the way to Better Ovarian Cancer Diagnosis
Aïcha BenTaieb will present her invention for automated identification of ovarian cancer’s many subtypes at an international conference this fall.
New Tech Enables Epigenomic Analysis with a Mere 100 Cells
A new technology that will dramatically enhance investigations of epigenomes, the machinery that turns on and off genes and a very prominent field of study in diseases such as stem cell differentiation, inflammation and cancer has been developed by researchers at Virginia Tech.
Futuristic Brain Probe Allows for Wireless Control of Neurons
NIH-funded scientists developed an ultra-thin, minimally invasive device for controlling brain cells with drugs and light.
Microfluidic Device Mixes And Matches DNA For Synthetic Biology
Researchers have developed a microfluidic device that quickly builds packages of DNA and delivers them into bacteria or yeast for further testing.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!