The Resealable Chip Interface is used in combination with microfluidic chips that can be opened and resealed. The holder clamps the layers together and provides eight fluidic and eight electrical connections to the chip. Two linear connectors 4-way (Part No. 3000148) are included. Reagents, sensors, biosensors or cells can be deposited on the chip base layer. The chip base layer material is typically glass, quartz, or polymer. Applications include: biosensor testing, cell culture and analysis, dielectrophoresis experiments and impedance detection.