|A Walk-Up ADME Testing System for Medicinal Chemists and Pharmacologists|
Muhammad Alimuddina, Martin Peacock, Russell Dahl, Omar Jina, and Paul Oakley
VivADME was born with the fusion of a problem and a solution: recognition by a Medicinal Chemist that ADME data took too long to obtain, and, the realization that microfluidic tools could address the problem. The vivADME microfluidics solution means that ADME profiling can be performed in minutes with microgram-scale samples in a walk-up system with a very low cost per sample.
|Functional Evaluation of SLC Transporters in BD Gentest™ Cryopreserved Human Hepatocytes Using an Oil-Filtration Suspension Assay|
1Eric Sands, 1Vina Ketty, 2Yong-Hae-Han, 1Joanne Bourgea, 1Chris J. Patten, 1Charles L. Crespi, and 1Guangqing Xiao
The purpose of this study was to evaluate the function of Sodium Taurocholate Co-transporting Polypeptide (NTCP), Organic Anion Transporting Polypeptide (OATP), and Organic Cation Transporter (OCT) in cryopreserved human hepatocytes, and to examine the impact of various drugs on hepatic transporter-mediated uptake. Cryopreserved human hepatocytes were prepared using the Percoll™ method.
|Bioluminescent Approaches for In Vitro ADMET|
James J Cali, Mary Sobol, Dongping Ma, Michael Valley
Bioluminescent ADMET assays couple targets of interest to the photon emitting reaction of firefly luciferase. Multiplexed cell-based assays measure compound cytotoxicity and induction of P450 transcription and enzyme activity. Cell-free membrane assays measure P450 or monoamine oxidase enzyme activities and their inhibition by test compounds.
|Initiatives For The Sharing Of Toxicity Data|
Much high-quality toxicity information is held within organisations rather than being in the public domain. Organisations would benefit from sharing this data with each other in a reciprocal manner where commercial sensitivity issues allow.
|Proteomic Approach to Identify Stage Specific Biomarkers in Streptozotocin Induced Diabetic Nephropathy|
Vikram Sharma and Kulbhushan Tikoo
The presence of albumin in the urine of healthy subjects presents a stumbling block in diagnostics putting a question mark on the validity of albumin test for the detection of diabetic nephropathy. Therefore there arises a dire need to hunt for the proteins in the pertinent bio- fluids which can serve as biomarkers.
|MS-Xelerator™: Advanced Algorithms for LC/MS Data Processing Applied to Biomarker Discovery, Differential Analysis and Quantitative Proteomics|
LC-MS based proteomic experiments are used to compare complex biological samples across multiple conditions. Fast, powerful computational tools are needed to explore and detect differences in the areas of Expression Proteomics and Biomarker Discovery. In general, specialized steps are necessary to solve these difficult problems (binning, alignment & normalization, peak picking, relative quantitation, etc.). MS-Xelerator is a collection of software tools dedicated to all of the above tasks.
|A Robust High Throughput Physicochemical Screen for Phospholipidosis|
Pavol Vitovic, Juha-Matti Alakoskela and Paavo K.J. Kinnunen
The poster presents a simple HT physicochemical screen, predicting PLD in real-time by surface activity profiling (using Kibron Delta-8 analyzer). The results are equal or better than derived from a cell based assay, throughput is up 450 compounds/24 hrs and drug consumption < 1 mg/compound.
|Promega’s Multiplexed Cell Viability and Apoptosis Assays Performed on the PHERAstar|
Tracy Worzella and Brad Larson
Today’s high-throughput screening facilities face increasing demands to generate more information from their existing compound libraries. In this poster, we demonstrate the combination of several Promega cell-based assays (CellTiter-Blue®, Apo-One® and Caspase-Glo® 3/7) multiplexed in both low-volume 384 and 1536-well plate formats. The BMG LABTECH PHERAstar microplate reader is used to record both luminescence and fluorescence, depending on the multiplex combination.
|Promega’s Multiplexed Luciferase Reporter and Cell Viability Assays performed on the PHERAstar|
Tracy Worzella and Brad Larson
By multiplexing a reporter assay (EnduRen™, ViviRen™) with a cell viability assay (CellTiter-Glo®), it is possible to determine if reporter response variations are due to changes in cell number and health. In this poster, we demonstrate the combination of several Promega cell-based assays multiplexed in both low-volume 384- and 1536-well plate formats using the BMG LABTECH PHERAstar microplate reader to record luminescence.