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Measurement of Residual Sugar in Wine Using the SpectraMax® Plus384 Microplate Reader
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Molecular Devices

Analysis of malic acid, residual sugar, volatile acidity and ammonia is very important in quality control during wine production. Enzymatic assays carried out in microplate format are quantitative and help achieve high throughput with respect totime and labor. The residual sugar assay involves enzymatic conversion of the analytes to give NADPH as a by-product. Measuring NADPH production by measuring absorbance at 340 nm allows direct quantitation of the analytes in wine samples. Here we describe the use of MDS Analytical Technologies’ SpectraMax® Plus384 microplate reader and SoftMax® Pro software to efficiently collect and analyze the data for enzymatic determination of residual sugar in wine.

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