Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Gut Microbes Signal to the Brain When They're Full
Don't have room for dessert? The bacteria in your gut may be telling you something.
Personalized Drug Screening for Multiple Myeloma Patients
A personalized method for testing the effectiveness of drugs that treat multiple myeloma may predict quickly and more accurately the best treatments for individual patients with the bone marrow cancer.
Nanocarriers May Carry New Hope for Brain Cancer Therapy
Berkeley lab researchers develop nanoparticles that can carry therapeutics across the brain blood barrier.
Cancer-Fighting Tomato Component Traced
The metabolic pathway associated with lycopene, the bioactive red pigment found in tomatoes, has been traced by researchers at the University of Illinois.
Batten Disease may Benefit from Gene Therapy
NIH-funded animal study suggests one-shot approach to injecting genes.
Shedding Light on “Dark” Cellular Receptors
UNC and UCSF labs create a new research tool to find homes for two orphan cell-surface receptors, a crucial step toward finding better therapeutics and causes of drug side effects.
Molecule Proves Key to Brain Repair After Stroke
Scientists found that a molecule known as growth and differentiation factor 10 (GDF10) plays a key role in repair mechanisms following stroke.
Scroll Up
Scroll Down

BlueScreen HC - A Luminescence Based, High-Throughput, In Vitro Genotoxicity Assay
Bookmark and Share


The BlueScreen HC assay utilizes a luciferase from the marine copepod Gaussia princeps to generate a luminescent output. Exposure to a genotoxic chemical causes increased expression of GADD45a and thus a dose dependent increase in the production of luciferase from the GADD45a reporter, which is naturally exported from the cell. The amount of luciferase produced by the cells is assessed by injection of a solution of the substrate, coelenterazine, resulting in a short-lived flash luminescence. In addition, reduced cell proliferation, a measure of cytotoxicity, is assessed either by changes in optical absorbance in the BlueScreen HC assay, or in fluorescence from a DNA stain in the BlueScreen HC S9 assay.

This application note shows how the BlueScreen HC and BlueScreen HC S9 assays are highly compatible with the BMG LABTECH FLUOstar Omega microplate reader, making use of the 3 principal detection modes and the reagent injection system.

Further Information


Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos