Corporate Banner
Satellite Banner
ADME Tox
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Rates of Nonmedical Prescription Opioid Use Disorder Double in 10 Years
Researchers at NIH have found that the nonmedical use of prescription opioids has more than doubled among adults in the United States from 2001-2002 to 2012-2013.
Self-Assembling Protein Shell for Drug Delivery
Made-to-order nano-cages open possibilities of shipping cargo into living cells or fashioning small chemical reactors.
Guided Chemotherapy Missiles
Latching chemotherapy drugs onto proteins that seek out tumors could provide a new way of treating tumors in the brain or with limited blood supply that are hard to reach with traditional chemotherapy.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
‘Human-on-a-Chip’ Could Replace Animal Testing
Researchers are developing a “human-on-a-chip,” a miniature external replication of the human body, integrating biology and engineering with a combination of microfluidics and multi-electrode arrays.
A New Approach to Chemical Synthesis
Communesins, originally found in fungus, could hold potential as cancer drugs.
‘Missing Tooth’ Hydrogels Handle Hard-to-Deliver Drugs
Rice University’s custom hydrogel traps water-avoiding molecules for slow delivery.
Copper is Key in Burning Fat
Berkeley Lab scientist says results could provide new target for obesity research.
Better Animal Model to Improve HIV Vaccine Development
Penn study identifies a new tool to produce better HIV vaccine designs.
Identifying Side-Effects At Early Stages Of Drug Development
An approach that could reduce the chances of drugs failing during the later stages of clinical trials has been demonstrated by a collaboration between the University of Cambridge and pharmaceutical company GlaxoSmithKline (GSK).
Scroll Up
Scroll Down

Analysis of a New High Throughput Screening Detection Technology for Rapid hERG Safety Testing using a Fluorescence Polarization Assay
Bookmark and Share

Biotek Instruments

Background
Speed and sensitivity are the hallmarks of Fluorescence Polarization (FP) assay technologies. Due to this FP has seen penetration in the drug discovery and drug development markets for High Throughput Screening applications. For a good overview of applications/target audiences for FP here is an easy to read and interesting White Paper with good citations for further reading: Fluorescence Polarization in Life Sciences. The Predictor hERG biochemical FP assay has been used as a model of FP performance validation used on our Synergy lines, not only because it is an assay designed for HTS run at low volume in 384-well plates, but because quantification of hERG channel binding and inhibition are cardio toxicity tests recommended for all drug compounds before human use (e.g. in pre-clinical trials). Therefore, the assay needs to prove it can compare with GLP-compliant gold standard methods, and the detection technology used to produce that data must be rigorous enough to do the same. Although BioTek has published a number of pieces of marketing collateral using the Predictor hERG assay, until Synergy Neo, other Synergy models could only provide sensitivity acceptable for detection of this assay at the cost of speed. Publication of optimal read parameters was either largely ignored or minimized in the other BioTek collateral for this reason, as the combination of assay and detection parameters required for optimal data were largely prohibitory for HTS applications when competitors could do it at ~ 2 min plate read time within a 2 hour assay incubation window.

Further Information

Related Content

BioTek Instruments and Global Cell Solutions set to Collaborate
BioTek Instruments, Inc., and Global Cell Solutions, Inc., (GCS) announce a formal collaboration for the coming year at LabAutomation 2010. The poster Automation of a Microplate Cell-based Assay to Measure Activity of the Histamine H1 G Protein-coupled Receptor Using a Novel 3-D Cell Culture Technique is the first in a series of collaborative publications detailing the use of BioTek’s microplate instrumentation for various cell-based assays.
Wednesday, January 27, 2010
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!