Corporate Banner
Satellite Banner
ADME Tox
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Oxygen Can Impair Cancer Immunotherapy
Researchers have identified a mechanism within the lungs where anticancer immune resposnse is inhibited.
Symmetry is Key to Collagen
Researchers describe how symmetry may be the key to growing collagen fibres outside the body.
Biological Barcodes Using CRISPR
Using genome editing tools, researchers are getting closer to understand differentiation of various cell types during development.
Decades Old Chemicals Linked to Current Increased Autism Risk
The chemcials - organochlorines - were banned in the US in 1977 but their side effects are still being seen.
Gene Therapy Via Ultrasound
Research into a gene therapy approach called sonoporation could help combat heart disease and cancer.
The Fight Against Fungal Infections
A pioneering university research team has recently employed such a system within their laboratory to obtain a greater understanding of the dynamics and progression of C. albicans in vivo using a mouse model.
Fly Study into Therapies for HVP-Induced Cancer
Fruit flies may help scientists understand the mechanism by which HPV can cause cancer as well as identify potential drug treatments.
Exploring Ebola-Malaria Link
Data shows people infected with Ebola were more likely to survive if co-infected with malarial parasite.
Precision Nanobots Target Cancerous Tumours
Researchers achieve breakthrough toward redefining anti-cancer drug administration using nanorobotics.
Characterizing the Mouse Gut Microbiome
Study establishes the first public collection of bacteria from the intestine of mice.
Scroll Up
Scroll Down

Rapid Critical Micelle Concentration (CMC) Determination Using Fluorescence Polarization
Bookmark and Share

BioTek Instruments

This application note describes the rapid semi-automated determination of CMC values for surfactants in 384-well microplates using fluorescence polarization. 

These data demonstrate that the use of DAF fluorescence polarization as a means to determine the CMC values for surfactants is not only easy and accurate, but that the method can also be easily scaled for large sample numbers. Unlike fluorescence intensity, fluorescence polarization uses a ratio of two measurements on each well, correcting for differences in intensity brought about by experimental conditions, such as pH, temperature, and surfactant concentration. The Synergy Neo reader is a high throughput reader specifically designed for the measurement of large numbers of samples. 

The reader uses modular optic cubes to measure numerous read modalities, which include UV-Vis absorbance, luminescence, fluorescence intensity, time resolved fluorescence, HTRF®, AlphaScreen®, and fluorescence polarization. In regards to fluorescence polarization, the reader is capable of simultaneously determining parallel and perpendicular measurements. Gen5™ software (BioTek instruments) not only controls reader function, but also is capable of automatically performing the 4-parameter logistic fit and calculating CMC values.


Further Information

Related Content

BioTek Instruments and Global Cell Solutions set to Collaborate
BioTek Instruments, Inc., and Global Cell Solutions, Inc., (GCS) announce a formal collaboration for the coming year at LabAutomation 2010. The poster Automation of a Microplate Cell-based Assay to Measure Activity of the Histamine H1 G Protein-coupled Receptor Using a Novel 3-D Cell Culture Technique is the first in a series of collaborative publications detailing the use of BioTek’s microplate instrumentation for various cell-based assays.
Wednesday, January 27, 2010
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!