Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Events>This Event
  Events - November 2014

3 days Hands-on qPCR

24 Nov 2014 - 26 Nov 2014 - Prague, Czech Republic

Bookmark and Share

3 DAYS HANDS-ON QPCR Target audience: Beginners to Medium experienced qPCR users Entrance qualifications: Basic Molecular biology or similar Description: The basic real-time qPCR course. You will aquire a comprehensive overview of the possibilities with real-time PCR, how to use it and how to analyze the results. The course contains: Day 1 Basic PCR theory The theory of real-time PCR Applications and possibilities of qPCR. Comparison of qPCR with regular PCR. Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons, etc) Different instrument platforms and their typical uses Primer design The problem of primer-dimer formation and how to minimize them Probe design Experimental design and optimization Basic data handling and analysis Day 2 Introduction to quantification principles Quantification strategies, uses and limitations Calculations using different relative quantification methods Strategies for normalization of qPCR data In situ calibration for compensation of inhibition in samples Absolute quantification Day 3 Sample Preparation (Extraction of RNA and DNA) Principles of RT Priming methods for RT RT optimization The MIQE guidelines Group discussion, bring your own questions

Further information
Scientific News
Photosynthesis Gene Could Help Crops Grow in Adverse Conditions
A gene that helps plants to remain healthy during times of stress has been identified by researchers at Oxford University.
Pancreatic Cancer Stem Cells Could be "Suffocated" by Anti-diabetic Drug
A new study shows that pancreatic cancer stem cells (PancSCs) are virtually addicted to oxygen-based metabolism, and could be “suffocated” with a drug already used to treat diabetes.
Scientists Learn How to Predict Plant Size
VIB and UGent scientists have developed a new method which allows them to predict the final size of a plant while it is still a seedling.
Scientists Home In On Origin Of Human, Chimpanzee Facial Differences
A study of species-specific regulation of gene expression in chimps and humans has identified regions important in human facial development and variation.
Nanoporous Gold Sponge Makes Pathogen Detector
Sponge-like nanoporous gold could be key to new devices to detect disease-causing agents in humans and plants, according to UC Davis researchers.
Genetic Manipulation for Algal Biofuel Production
Studies of the genes involved in oil synthesis in microalgae allow scientists to use a gene promoter to increase algal production of triacylglycerols, which in turn enhances potential biofuel yields.
Phosphorous Fertilizer
UD researchers identify behaviors of nanoparticle that shows promise as nanofertilizer.
Marijuana Genome Unraveled
A study by Canadian researchers is providing a clearer picture of the evolutionary history and genetic organization of cannabis, a step that could have agricultural, medical and legal implications for this valuable crop.
Grape Waste Could Make Competitive Biofuel
The solid waste left over from wine-making could make a competitive biofuel, University of Adelaide researchers have found.
Accelerating Forage Breeding to Boost Livestock Productivity
International expert skill-sets in genomics and bioinformatics enhance our capacity to breed improved forages for Africa.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos