Corporate Banner
Satellite Banner
AgriGenomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

High-throughput Cryopreservation of Plant Cell Cultures for Functional Genomics

Published: Tuesday, April 10, 2012
Last Updated: Tuesday, April 10, 2012
Bookmark and Share
Here, scientists have developed a simple protocol for cryopreservation of suspension-cultured cells from five commonly used plant species—A. thaliana, Daucus carota, Lotus japonicus, Nicotiana tabacum and Oryza sativa.

Abstract

Suspension-cultured cell lines from plant species are useful for genetic engineering. However, maintenance of these lines is laborious, involves routine subculturing, and hampers wider use of transgenic lines, especially when many lines are required for a high-throughput functional genomics application. Cryopreservation of these lines may reduce the need for subculturing. Here, we established a simple protocol for cryopreservation of cell lines from five commonly used plant species, Arabidopsis thaliana, Daucus carota, Lotus japonicus, Nicotiana tabacum, and Oryza sativa. The LSP solution (2 M glycerol, 0.4 M sucrose, and 86.9 mM proline) protected cells from damage during freezing and was only mildly toxic to cells kept at room temperature for at least two hours. More than 100 samples were processed for freezing simultaneously. Initially, we determined the conditions for cryopreservation using a programmable freezer; we then developed a modified simple protocol that did not require a programmable freezer. In the simple protocol, a thick expanded-polystyrene (EPS) container containing the vials with the cell/LSP solution mixtures was kept at -30°C for 6 hours to cool the cells slowly (prefreezing); samples from the EPS containers were then plunged into liquid nitrogen before long-term storage. Transgenic Arabidopsis cells were subjected to cryopreservation, thawed, and then re-grown in culture; transcriptome and metabolome analyses indicated that there was no significant difference in gene expression or metabolism between cryopreserved cells and control cells. The simplicity of the protocol will accelerate the pace of research in functional plant genomics.

This article was published online in Plant and Cell Physiology and is free to access.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,800+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Arabidopsis TOE Proteins Involved in the Regulation of Flowering Time
This study found that TOE proteins serve as major integrator of developmental and environmental signaling pathways, especially the photoperiod flowering pathway, triggering flowering at an appropriate age and time.
Wednesday, May 06, 2015
Aflatoxin Biosynthesis - a Novel Source of Reactive Oxygen Species
Researchers at the Michigan State University found that the aflatoxin biosynthetic pathway in A. Parasiticus directly or indirectly generates ROS in aflatoxisomes/endosomes and that Superoxide represents a significant fraction of the aflatoxin biosynthesis-derived ROS pool.
Wednesday, May 06, 2015
SSR Markers and Genetic Diversity in White Birch
The results of this study indicate that the white birch trees sampled from six geographical locations had low to moderate similarity (0.025–0.610) and suggested that the SSR primers used in this study can effectively distinguish white birch germplasm.
Wednesday, May 06, 2015
The Genetics Behind Abdominal Pigmentation in Drosophila melanogaster
This study by researchers at the North Carolina State University provides evidence that genetic variation at a number of steps in regulatory, developmental, and transport pathways may contribute to natural variation in abdominal pigmentation.
Wednesday, May 06, 2015
Mass Spec-Based Metabolomics Workflows to Evaluate Plant Immunity
This article reviews metabolomics studies used to evaluate plant immunity with an emphasis on mass spectrometry in its many forms.
Friday, July 25, 2014
Determination of Arabidopsis Thaliana Telomere Length by PCR
Researchers have designed two telomeric degenerated primers that amplify Arabidopsis telomeres by MMQPCR.
Friday, July 11, 2014
FTIR Spectroscopy as a Method to Study Lipid Accumulation in Oleaginous Yeasts
The absorption spectra allows monitoring of the progression of fatty acid accumulation and identification of the most represented classes of the produced lipids.
Tuesday, February 11, 2014
Optical Biosensors for Food Quality and Safety Assurance
With high specificity, convenience and quick response there is growing interest in biosensors for food safety testing.
Monday, October 07, 2013
RNA-Seq Reveals Infection-Related Gene Expression Changes in Phytophthora capsici
This study provides a critical step to characterize the mechanisms of pathogenicity and virulence of P. capsici.
Thursday, September 12, 2013
Simultaneous Saccharification and Co-Fermentation for Bioethanol Production at Lab, PDU and Demo Scales
In this study, researchers designed a SSCF process and scaled up from lab to demo scale reaching 4% (w/v) ethanol using xylose rich corncobs.
Monday, February 04, 2013
DNA Microarrays of GM Crops
Non-uniform distribution pattern for differentially expressed genes of transgenic rice Huahui 1 at different developmental stages and environments.
Wednesday, May 23, 2012
Disruption of a Rice PPR Protein Causes a Seedling-Specific Albino Phenotype and its Utilization to Enhance Seed Purity in Hybrid Rice Production
Great efforts have been made to minimize the environmental effect in hybrid rice production. However, previous attempts using leaf color mutants as screening-markers to monitor seed purity in two-line hybrid rice production have achieved limited success.
Thursday, March 22, 2012
A Novel Biochemical Route for Fuels and Chemicals Production from Cellulosic Biomass
This study proposes an alternative route for fuels and chemical production, which combines cellulase production and enzymatic hydrolysis into a single biological step and produces sugar aldonates instead of sugars as the reactive intermediates.
Thursday, March 08, 2012
Recent Advances of Metabolomics in Plant Biotechnology
This article describes the basic analytical advancements in plant metabolomics and bioinformatics and the application of metabolomics to the biological study of plants.
Thursday, February 16, 2012
Scientific News
Flowers Arrange Themselves for Bees
Study suggests plants can maximise their chances of reproduction by taking advantage of how insects move when they gather nectar.
Improving Wheat Crops in the Field
Agrii, RAGT and the University of Nottingham are developing better disease management and yield production in wheat crops using ASD FieldSpec Handheld 2 portable spectroradiometers.
Unravelling the Roots of Insect’s Waterproof Coating
Researchers have identified the genes that control cuticular lipid production in Drosophila, by performing an RNAi screen and using Direct Analysis in Real Time and GC-MS.
Structural link to Brain Cell Death in Alzheimer's
Study reveals multiple new leads for pursuing potential Alzheimer's treatments
Disentangling the Plant Microbiome
Study says breeding plants, to feed a growing global population, with more beneficial bacteria is far from simple.
Cellular Origin of Skin Cancer Identified
Scientists have identified ‘cell of origin’ in the most common form of skin cancer, and followed the process that leads to tumour growth.
How Plants Sense Electric Fields
An international group of researchers has identified the sensor plants use to sense electric fields. The voltage sensor discovery could contribute to the understanding of how the Ebola virus enters human cells.
Google and EI Partner for Next Generation of Coders
The Earlham Institute's open-source project for visualisation of biological data BioJS acts as mentor organisation for Google Summer of Code 2016.
DNA Production Facility Begins Operation
Scientists mark the opening of the UK's first fully automated DNA construction and modification facility.
A 3D Paper-Based Microbial Fuel Cell
Researchers have developed a proof-of-concept 3D paper-based microbial fuel cell (MFC) that could take advantage of capillary action to guide the liquids through the MFC system and to eliminate the need for external power.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!