Corporate Banner
Satellite Banner
AgriGenomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Biolog's Phenotype MicroArray Technology Employed by the UK AHVLA

Published: Friday, January 11, 2013
Last Updated: Friday, January 11, 2013
Bookmark and Share
Technology enabled scientists to make breakthrough discoveries on the tuberculosis bacterium.

In a paper published today in the journal PLOS ONE, a  research team headed by Dr. Paul Wheeler from the Animal Health and Veterinary Laboratories Agency (AHVLA, Weybridge, UK) reported breakthrough progress in understanding the metabolic and phenotypic properties of the bacterium Mycobacterium tuberculosis and its close relative, Mycobacterium bovis.  Link to article: http://dx.plos.org/10.1371/journal.pone.0052673

Mycobacterum tuberculosis is the causative agent of the respiratory disease tuberculosis which infects an estimated 8 million people worldwide and is responsible for 2 million fatalities each year. Tuberculosis is a transmissible respiratory disease and as such represents a major problem and focus of public health efforts around the world.  Mycobacterium bovis afflicts cattle with losses to agriculture of approximately $3 billion per year. These mycobacteria have been very difficult for scientists to study, because they grow very slowly, so experiments can take weeks or months to perform.

The publication from the AHVLA is important in several respects. First, it shows that Biolog's Phenotype MicroArray™ (PM) technology allows these bacteria to be studied much more quickly and easily, which will accelerate the pace of mycobacterial research. Results can be obtained in 7 to 10 days. Second, it demonstrates diagnostic potential by phenotypically differentiating strains of these mycobacteria with different  host ranges and levels of pathogenicity. Third, the paper expands, as well as confirms, our knowledge of the metabolic properties of these mycobacteria. As a consequence, genome annotation can be improved, the biology of these bacteria can be better understood, and hopefully these insights will facilitate discovery of antibiotics more effective in their eradication.

According to Dr. Wheeler, "The genome sequence of Mycobacterium tuberculosis was published in 1998 and high-throughput phenotype analysis of pathogenic mycobacterial strains is urgently needed and long overdue. Molecular typing of Mycobacterium strains has limitations. Though key in surveillance and helpful in identifying emerging strains, it does not provide information on biological properties or phenotypes. This is a substantial gap in our knowledge since it is the phenotype which is selectable and must relate to the evolutionary advantage of one strain over another."

Other mycobacterial species have also been successfully studied with PM technology.  In June of this past year, researchers in the laboratory of Prof. Yung-Fu Chang at Cornell University College of Veterinary Medicine published also in PLOS ONE on their use of PM technology to analyze the metabolic phenotypes of Mycobacterium avium.  In 2009, a team of researchers in the laboratory of Prof. Lacy Daniels at Texas A&M, Kingsville used gene knockouts combined with PM technology to show that the Mycobacterium smegmatis gene homolog of the Mycobacterium tuberculosis gene Rv1238 codes for a transporter of the sugar trehalose and plays a critical role in pathogenicity. Additionally, in a paper just published January 4, 2013 online in the Journal of Bacteriology, Prof. Daniels' lab again analyzes the phenotypes of gene knockouts with PM technology to define the spectrum of antibiotics and antiseptics for mycobacterial efflux pumps. Antibiotic resistance is another focus of mycobacteria research.

Biolog PM technology has now enabled multiple important discoveries with mycobacteria. “We are thrilled that three laboratories have now successfully applied Biolog’s PM technology in pioneering research resulting in breakthrough discoveries” said Dr. Barry Bochner, CEO & CSO at Biolog, Inc. (Hayward, CA). “PM technology is designed to provide high throughput phenotyping and metabolic scanning of cells, making it a powerful complement to genotyping experiments.”

Phenotype MicroArray technology, initially developed with SBIR funding from NIH, is proving to be a cell profiling technology that can yield breakthrough discoveries. It allows scientists to study the growth properties and culture condition responses of bacterial, fungal, and even human cells. As such it is becoming a core technology for many cellular studies.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Flowering Regulation Mechanism Discovered
Monash researchers have discovered a new mechanism that enables plants to regulate their flowering in response to raised temperatures.
Nanoparticles Present Sustainable Way to Grow Food Crops
Nanoparticle technology can help reduce the need for fertilizer, creating a more sustainable way to grow crops such as mung beans.
Analysis of Dog Genome will Provide Insight into Human Disease
An important model in studying human disease, the non-coding RNA of the canine genome is an essential starting point for evolutionary and biomedical studies – according to a new study led by The Genome Analysis Centre (TGAC).
Pathogen Takes Control of Gypsy Moth Populations
A new fungal pathogen is killing gypsy moth caterpillars and crowding out communities of pathogens and parasites that previously destroyed these moth pests.
Super Wheat Brought Closer to Reality
Scientists at the John Innes Centre (JIC) and The Sainsbury Laboratory (TSL) have pioneered a new gene-detecting technology which, if deployed correctly could lead to the creation of a new elite variety of wheat with durable resistance to disease.
Mechanism Behind Plant Withering Clarified
Reproducing the reaction in which harmful reactive oxygen species are created during plant photosynthesis allows researchers to confirm the mechanism behind plant withering.
Sequencing the Salmon Genome
Researchers have established a “human” quality sequence of the Atlantic salmon genome that is now available online.
Improved Path to Cassava Production
Researchers have studied the genetic diversity of cassava, highlighting strategies to improve breeding programmes.
New Online Tool Helps Predict Gene Expression in Plants
Scientists at The Genome Analysis Centre (TGAC) and The John Innes Centre have developed a free online tool that will help a global community of scientists understand more about important food crops.
Rare DNA Transfer Between Animals, Plants
Scientists identify rare DNA transfer between conifers and insects.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!