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Functional Analysis of RAD51B and RAD23B SNPs using Circular Chromosome Conformation Capture (4C) in Human Prostate Cell Lines
C. Cieza-Borrella1, S. Wakerell1 , Q. Karlsson1 , R.A. Eeles1,2, Z. Kote-Jarai1

The aim of this study is to understand the mechanism behind the association between single nucleotide polymorphisms (SNPs), lying in non-coding regions near or within RAD51B and RAD23B, and prostate cancer risk by analysing their interactions with the whole genome using Circular Chromosome Conformation Capture (4C).

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CRISPR-Cas9 genome editing utilizing chemically synthesized RNA
Michael Delaney, Kaizhang He, Eldon Chou, Amanda Haas, Žaklina Strezoska, Melissa L. Kelley, and Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA

Chemical synthesis has been easily applied for rapidly generating either crRNA and tracrRNA or synthetic sgRNA for direct delivery into cells for gene editing applications such as DNA-free options and high throughput arrayed screening.

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The Chemical Synthesis of Long and Highly Modified RNA using 2'-ACE Chemistry
Xiaoqin Cheng, Kristina Larson, Letitia Kwok, David Mierzejewski, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney, David Kitchen, Amanda Haas, Melissa Kelley, Anja van Brabant Smith

Dharmacon has previously developed a novel RNA synthesis chemistry making RNA synthesis as reliable, accessible and of comparable quality as routinely observed in DNA synthesis.

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Blood Transcriptomic Diagnosis of Active TB with Five Genes
JK Roe1*, N Thomas1, E Gil1, K Best1, E Tsaliki1, S Morris-Jones2, S Stafford1, N Simpson1, KD Witt3, B Chain1, R Miller4, A Martineau3, M Noursadeghi1

This study aimed to identify the minimal set of genes that can accurately diagnose active TB, both pulmonary and extrapulmonary, and evaluate their specificity.

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Experimental design considerations for efficient and specific gene knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA
Hidevaldo B. Machado, John A. Schiel, Maren Mayer-Gross, Eldon T. Chou, Melissa L. Kelley, Anja van Brabant Smith. Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA

Precise genome engineering with CRISPR-Cas9 and single-stranded DNA oligo or double-stranded DNA plasmid donors via homology-directed repair (HDR).

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Super-resolution single molecule localization microscopy of the exocytotic machinery underlying insulin secretion.
Deirdre M. Kavanagh, Alison Dun, Rory R. Duncan, and Colin Rickman.

Single molecule imaging of the tSNARE proteins involved in insulin secretion.

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Resistance-Associated Variants in HCV Genotype 1 Populations
Elian Rakhmanaliev1, Pramila Ariyaratne1, Zhang Rui1, Kok Siong Poon2, Cui Wen Chua2, Mui Joo Khoo2, Evelyn S. Koay2, Ekawat Passomsub3, Wasun Chantratita3, Gerd Michel1

In this study we specifically investigated RAVs emerging in HCV genotype 1.

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Integrated Next-Generation Sequencing and qPCR Workflow in Clinical Diagnostics
Elian Rakhmanaliev, Tatiana Ivanova, Gerd Michel

Combined automated qPCR and NGS Sentosa workflow is a reliable and efficient in vitro diagnostics (IVD) tool for the detection and/or quantitation of a wide range of bacterial and viral pathogens as well as gene mutations.

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A Synthetic CRISPR-Cas9 System for Homology-directed Repair
John A. Schiel, Maren M. Gross, Emily M. Anderson*, Eldon T. Chou, Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA

Synthetic, dual-RNA-encoded Cas9 is used for precise homology-directed repair (HDR) gene engineering. Both short and long (GFP) inserts are covered.

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CRISPR-Cas9 genome editing utilizing chemically synthesized RNA
Kaizhang He, Eldon Chou, Amanda Haas, Žaklina Strezoska, Melissa L. Kelley, and Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA

CRISPR-Cas9 gene editing using synthetic crRNA:tracrRNA or sgRNA is highly efficient and easy to use. Synthetic crRNA:tracrRNA is uniquely suited to in vitro and in vivo applications, in particular, DNA-free approach with Cas9 mRNA. Chemical synthesis of guide RNAs allows accurate and rapid production of arrayed crRNA libraries for high-confidence, loss-of-function screens.

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Scientific News
Plant Analysis – Identifying Metabolites
New plant analysis method shows biologically active plant substances are far more common than previously thought.
Exploring the Genome of the River Blindness Parasite
Researchers have decoded the genome of the parasite that causes the skin and eye infection known as river blindness.
Gene Editing Yields Tomatoes That Ripen Weeks Earlier
Research team develop method to make tomato plants flower and ripen fruit two weeks faster than current growth rates.
Gene-Editing Improves Vision in Blind Rats
Scientists developed a targeted gene-replacement technique that can modify genes in both dividing and non-dividing cells in living animals.
BGI Sequences Gingko Tree, Revealing Large, Highly Repetitive Genome
Researchers at BGI have sequenced the more than 10-gigabase ginkgo genome to find a high number of repetitive sequences as well as a number of gene clusters that appear to be involved in defense mechanisms.
Biologists Discover Origin of Stomata
Researchers discover genetic mechanism similar in flowering plants and mosses is a result of evolutionary conservation.
Uncovering a World of Viruses
Study that shows human diseases like influenza are derived from those present in invertebrates.
Engineering Bacteria to Aid Ethanol
Splicing in genes for ethanol production into bacteria in order to produce ethanol rather than not lactic acid.
Controlling Cell Division in Plants
Researchers succeeded in developing a new compound, a triarylmethane, that can rapidly inhibit cell division in plants.
Plant Aging Study Produces Insights into Crop Yields
New insights into the mechanism behind how plants age may help scientists better understand crop yields and nutrient allocation.
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