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CRISPR-Cas9 genome editing utilizing chemically synthesized RNA
Kaizhang He, Eldon Chou, Amanda Haas, Žaklina Strezoska, Melissa L. Kelley, and Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA

CRISPR-Cas9 gene editing using synthetic crRNA:tracrRNA or sgRNA is highly efficient and easy to use. Synthetic crRNA:tracrRNA is uniquely suited to in vitro and in vivo applications, in particular, DNA-free approach with Cas9 mRNA. Chemical synthesis of guide RNAs allows accurate and rapid production of arrayed crRNA libraries for high-confidence, loss-of-function screens.

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The Case for CASE: Computer-Assisted Structure Elucidation
ACD/Labs

Modern CASE systems such as Structure Elucidator Suite provide the necessary capability accurately elucidate a novel chemical structure for complex molecules based on readily available NMR data sets. This allows organizations to avoid expensive, labor-intensive, and time-consuming synthetic efforts.

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A Unified Software Platform for Laboratory Informatics
Graham A. McGibbon, Hans de Bie, David Hardy, Ryan Sasaki, Patrick Wheeler, Carol Preisig

Reported here are capabilities in automated workflows involving analytical data with chemical structures. Specifically described is automated homogenization of data from a set of instruments, including NMR structure verification, as one solution.

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Gene Expression Patterns in Donor-matched Mesenchymal Stem Cells and Osteocytes from Human Trabecular Bone
Dragos Ilas, Sarah Churchman, Peter Giannoudis, Dennis McGonagle and Elena Jones

The study aimed to investigate gene expression profiles of osteocytes in healthy and OA bone and to compare with donor - matched MSCs.

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Rapid electrochemical impedance spectroscopy for protein detection in Lab-on-a-Chip devices
T. Pardy(a); N. Sleptsuk(a); M. Min(a); J. Ojarand(a); T. Lakspere(a,b); K. Palm(b)

We present results in rapid solution impedance spectroscopy to detect protein interactions (antibody-antigen) in human serum. Two different serum-antibody mixtures are measured in cuvettes, interfaced with screen-printed electrodes to determine whether the experimental setup can detect differences in composition.

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Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics
Shubha Jain, F. Azam, Dr. H. N. Unni

We have developed and characterized a micro-fluidic mixer to study the macro-molecule dynamics such as kinetics of protein folding, DNA sequencing, single molecule study and detection etc. on a micro-second timescale. Numerical simulation has been performed to analyse the study of mixing performance of micro-fluidics channel.

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Analysis of Terpenes Using Gas Chromatography with Vacuum Ultraviolet
Changling Qiu, Jonathan Smuts, Phillip Walsh, and Kevin A. Schug

The VUV absorption spectra for different terpenes were distinctive and differentiable. GC-VUV demonstrated the capabilities for qualitative and quantitative analysis of terpenes in turpertine mixtures. Chromatographic coeluting signals can be deconvolved by the VUV data analysis software.

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Assessing Diversity in Cassava through the Application of Metabolomics
Margit Drapala, Elisabete Carvalhoa, Laura Perez-Fonsa, Elliott Pricea, L. Augusto Becerra Lopez-Lavalleb, Paul D. Frasera

In the present study metabolomic platforms have been established for Cassava and used to assess the biodiversity present in Cassava germplasm collections and elucidate underlying biochemical mechanisms associated with traits of interest.

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Metabolic response to everolimus in patient-derived xenografts of triple negative breast cancer
Leslie R. Euceda1, Deborah K. Hill1,2, Endre Stokke1, Elisabetta Marangoni3, Tone F. Bathen1, Siver A. Moestue1,2

Everolimus treatment metabolic effects on TNBC PDX models were investigated using MR spectroscopy. PLS-DA successfully discriminated treated from controls and PDX expressing INPP4B and not. LMM revealed significant differences in 4/17 metabolites and two metabolite ratios between treated and controls.

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Design considerations for highly specific and efficient synthetic crRNA molecules
Anja van Brabant Smith, Emily M. Anderson, Shawn McClelland, Elena Maksimova, Tyler Reed, Steve Lenger, Žaklina Strezoska, Hidevaldo Machado Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Suite #100, Lafayette, CO 80026, US

An overview of our rational design algorithm for picking highly functional crRNA sequences in combination with comprehensive specificity analysis.

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Scientific News
World’s Largest Coral Gene Database
‘Genetic toolkit’ will help shed light on which species survive climate change.
A Peachy Defense System for Seeds
ETH chemists are developing a new coating method to protect seeds from being eaten by insects. In doing so, they have drawn inspiration from the humble peach and a few of its peers.
Roundup Impacts Gene Expression
Study published on the impact of low-dose toxicity of Roundup weed-killer on gene expression profiles.
Meaningful Part of Maize Genome Defined
FSU-Cornell team show that a small percentage of the maize genome is responsible for 40 percent of a plant’s trait diversity.
Plant Stem Cell Discovery Points to Increased Yields
Braking signals from the leaves tell stem cells to stop proliferating.
Plasma Dose Improves Agricultural Crop Harvests
Researchers at Japan have developed a technique to improve crop yields by treating seeds prior to planting with a safe dose of plasma radiation.
TGAC Installs Largest SGI UV 300 Supercomputer for Life Sciences
The Genome Analysis Centre (TGAC) partners with Global HPC hardware giant SGI to address the most complex problems in genomics analysis.
Carrot Genome Uncovered
Carrot genome paints picture of domestication, could help improve crops.
Flowering Regulation Mechanism Discovered
Monash researchers have discovered a new mechanism that enables plants to regulate their flowering in response to raised temperatures.
Nanoparticles Present Sustainable Way to Grow Food Crops
Nanoparticle technology can help reduce the need for fertilizer, creating a more sustainable way to grow crops such as mung beans.
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