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The Case for CASE: Computer-Assisted Structure Elucidation
ACD/Labs

Modern CASE systems such as Structure Elucidator Suite provide the necessary capability to accurately elucidate a novel chemical structure for complex molecules based on readily available NMR data sets. This allows organizations to avoid expensive, labor-intensive, and time-consuming synthetic efforts.

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A Unified Software Platform for Laboratory Informatics
Graham A. McGibbon, Hans de Bie, David Hardy, Ryan Sasaki, Patrick Wheeler, Carol Preisig

Reported here are capabilities in automated workflows involving analytical data with chemical structures. Specifically described is automated homogenization of data from a set of instruments, including NMR structure verification, as one solution.

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Customizable exon-centric target enrichment strategy for copy number and SNP analysis
Arjun Vadapalli*, Kyeong Soo Jeong*, Ashutosh Ashutosh, Devendra Joshi , Eric Lin, Carlos Pabon, Gilbert Amparo, Jayati Ghosh, Douglas Roberts *Equally contributed

Agilent’s Custom OneSeq provides a comprehensive, flexible, and cost-effective means to identify exon-level copy number changes as well as SNP/INDEL in one assay.

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Rapid electrochemical impedance spectroscopy for protein detection in Lab-on-a-Chip devices
T. Pardy(a); N. Sleptsuk(a); M. Min(a); J. Ojarand(a); T. Lakspere(a,b); K. Palm(b)

We present results in rapid solution impedance spectroscopy to detect protein interactions (antibody-antigen) in human serum. Two different serum-antibody mixtures are measured in cuvettes, interfaced with screen-printed electrodes to determine whether the experimental setup can detect differences in composition.

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Advanced Microfluidic Mixing Device for the Study of Macromolecule Dynamics
Shubha Jain, F. Azam, Dr. H. N. Unni

We have developed and characterized a micro-fluidic mixer to study the macro-molecule dynamics such as kinetics of protein folding, DNA sequencing, single molecule study and detection etc. on a micro-second timescale. Numerical simulation has been performed to analyse the study of mixing performance of micro-fluidics channel.

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DNA-free CRISPR-Cas9 genome engineering in zebrafish
Amanda Haas, Alex J. Blasky*, Rytis Prekeris*, John A. Schiel, Melissa L. Kelley, and Anja van Brabant Smith | Dharmacon, now part of GE Healthcare, 2650 Crescent Drive, Suite 100, Lafayette, CO 80026, USA *University of Colorado - Anschutz Medical Campus, Department of Cell & Developmental Biology, Denver, CO, USA

Poster describing the advantages of a DNA-free gene editing system and the application of this system in zebrafish.

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Analysis of Petroleum Products Using Comprehensive Two-Dimensional Gas Chromatography (GCxGC) with Both Time-of-Flight MS and Flame Ionization Detectors
Christina N. Kelly, Jonathan D. Byer, David E. Alonso, Lorne E. Fell, Joseph E. Binkley

Several possible configurations were tested to develop a workflow which would allow either subsequent or simultaneous acquisitions of GC×GC TOFMS and FID data on the same GC×GC instrument for petroleum analysis.

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Analysis of Terpenes Using Gas Chromatography with Vacuum Ultraviolet
Changling Qiu, Jonathan Smuts, Phillip Walsh, and Kevin A. Schug

The VUV absorption spectra for different terpenes were distinctive and differentiable. GC-VUV demonstrated the capabilities for qualitative and quantitative analysis of terpenes in turpertine mixtures. Chromatographic coeluting signals can be deconvolved by the VUV data analysis software.

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600 base reads on the Ion S5™ Next-Generation Sequencing System enables accurate HLA typing of 96 samples on one 530™ chip
Peter B. Vander Horn, Cisilya Duncan, Jamsheed Ghadiri, Amneet Gulati, Diana Jeon, April Jung, Mindy Landes, Tommie Lincecum, Geoffrey Lowman, Vadim Mozhayskiy, Linus Ong, Xinzhan Peng, Maryam Shenasa, Prasanna Thwar

We have demonstrated that by combining improvements in templating and sequencing biochemistry we are able to sequence templates longer than 600 bases with high accuracy on an Ion S5 530 chip.
These improvements open the S5 use space to include haplotyping applications that require longer reads. As a demonstration of that, we accurately typed 96 HLA samples on one 530 chip.

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Scientific News
Plant Analysis – Identifying Metabolites
New plant analysis method shows biologically active plant substances are far more common than previously thought.
Exploring the Genome of the River Blindness Parasite
Researchers have decoded the genome of the parasite that causes the skin and eye infection known as river blindness.
Gene Editing Yields Tomatoes That Ripen Weeks Earlier
Research team develop method to make tomato plants flower and ripen fruit two weeks faster than current growth rates.
Gene-Editing Improves Vision in Blind Rats
Scientists developed a targeted gene-replacement technique that can modify genes in both dividing and non-dividing cells in living animals.
BGI Sequences Gingko Tree, Revealing Large, Highly Repetitive Genome
Researchers at BGI have sequenced the more than 10-gigabase ginkgo genome to find a high number of repetitive sequences as well as a number of gene clusters that appear to be involved in defense mechanisms.
Biologists Discover Origin of Stomata
Researchers discover genetic mechanism similar in flowering plants and mosses is a result of evolutionary conservation.
Uncovering a World of Viruses
Study that shows human diseases like influenza are derived from those present in invertebrates.
Engineering Bacteria to Aid Ethanol
Splicing in genes for ethanol production into bacteria in order to produce ethanol rather than not lactic acid.
Controlling Cell Division in Plants
Researchers succeeded in developing a new compound, a triarylmethane, that can rapidly inhibit cell division in plants.
Plant Aging Study Produces Insights into Crop Yields
New insights into the mechanism behind how plants age may help scientists better understand crop yields and nutrient allocation.
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