|Low-cost wind tunnel for micro-arthropods|
Rostislav Zemek and Frantisek Reindl
Here we describe a construction of wind tunnel suitable for close-up observation of micro-arthropods behavior under various conditions. The tunnel was made of glass with inner dimensions 6x6x110 centimeters. Both the inlet and outlet compartments were equipped with an electric fan, series of plastic diffusers and a metal mesh to minimize wind turbulence inside a 40 centimeters-long observation chamber in the middle of the tunnel.
|The first record of Beauveria bassiana (Deuteromycetes) on the hibernating pupae of Cameraria ohridella (Lepidoptera: Gracillariidae)|
Eva Prenerová, Rostislav Zemek, František Weyda
Samples of the horse chestnut leaves with C. ohridella pupae were collected in autumn at Ceske Budejovice. The leaves were dissected and the pupae were individually placed into the 23°C and 95% R.H. Development of mycosis was frequently observed but only a few pupae were infected by B. bassiana. The strains of the fungus were isolated from individual pupae and deposited in Culture Collection of Entomopathogenic Fungi Olesna.
|Evaluation of entomopathogenic fungi as a biological control agent of Cameraria ohridella, an invasive pest of Aesculus hippocastanum in Europe.|
Rostislav Zemek, Eva Prenerová, František Weyda, Lubomír Volter
We evaluated the potential for using entomopathogenic fungi to control the horse chestnut leaf-miner, Cameraria ohridella Deschka et Dimic (Lepidoptera, Gracillariidae). The laboratory bioassays revealed a high insecticidal activity of I. fumosorosea blastospores and conidia on both C. ohridella pupae and eggs.
|Entomopathogenic fungi isolated from soil in the vicinity of Cameraria ohridella - infested horse chestnut trees|
Eva Prenerová, Rostislav Zemek, František Weyda, Lubomír Volter
Occurrence of entomopathogenic fungi in soil samples collected near horse chestnut trees infested by its pest, Cameraria ohridella, was surveyed using the modified Galleria bait method. The results revealed that entomopathogenic fungi frequently occur in the soil samples. Dominant species found were Isaria fumosorosea and Beauveria bassiana. The isolated strains are deposited in the Culture Collection of Entomopathogenic Fungi Olešná in the Czech Republic.
|Tapping into the mechanical links of expansins in leaf morphogenesis|
Expansins are conserved family of non-enzymatic proteins involved in cell wall-loosening accounted for widespread role in plant development. My research focuses on functional analysis of expansins during leaf morphogenesis. Atomic force microscopy was applied to characterise cell wall mechanical properties in an attempt for in vivo quantification of expansin activity. It’s showed how that there is a distinct difference in cell wall extensibility between leaf abaxial and adaxial side.
| A major gene for grain cadmium accumulation in soybean (Glycine max [L] Merr)|
Eduardo Rodriguez Benitez1, Makita Hajika1, Tetsuya Yamada1, Koji Takahashi1 and Nobuhiko Oki1
Quantitative Trait Loci (QTL) analyses were conducted in a population derived from a cross of varieties which differ in their seed Cd accumulation. Results suggested that seed Cd accumulation might be controlled by one major gene
|DNA polymorphism in the pepper-Phytophthora capsici pathosystem|
Cristina Fernández-Otero1,2, Jesús Moreno-González1, and James P. Prince2
Phytophthora capsici is an important soilborne pathogen that produces a devastating disease on many crop species, including pepper. To analyze DNA polymorphism in the P. capsici-pepper pathosystem, six C. annuum lines with various degrees of resistance to P. capsici: Padrón, Couto,CM334,PI201234, SP-11 and JEP; and two P. capsici isolates,GSP1-1 and Ppc3, selected for different levels of virulence, were tested with a variety of molecular markers
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