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A Novel Approach Toward Microfluidic Drug Metabolite Synthesis – Electrosynthetic Methodology Simulating Cytochrome (CYP450) Oxidation
Romain Stalder, Gregory P. Roth and Philip Podmore

A novel microfluidic technology and electrochemical synthesis method is demonstrated for the efficient generation of known drug metabolites. These metabolites are typically generated on first pass hepatic oxidation in vivo. The FLUX Module, a new microfluidic electrochemical cell manufactured by Syrris Ltd., has been employed to generate the metabolites of five commercial drugs: Tolbutamide, Chlorpromazine, Diclofenac, Primidone and Albendazole.

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A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing
Christian Le Cocq, Kyeong Soo Jeong, Arjun Vadapalli, Joseph Ong, Elin Agne, Filip Karlsson, Ashutosh Ashutosh, Francisco Useche, Jayati Ghosh, Henrik Johansson, Scott Happe, Douglas Roberts, and Holly Hogrefe

Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.

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Mutation Induction in Sucrose Synthase 1 to Study Cold Acclimation in Winter Wheat
Rita Armoniene, Gintaras Brazauskas

Sucrose synthase (Ss) catalyzes the reversible conversion of sucrose and a nucleoside diphosphate into the corresponding nucleoside diphosphate-glucose and fructose being one of the main enzymes of carbohydrate metabolism. The objectives of this study were: to create a TILLING population in winter wheat; to identify new alleles of Ss1 gene; to compare relative expression of Ss1 in leaves and crowns of mutant versus wild type plants during cold acclimation.

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Characterization of the prehaustorial resistance against leaf rust (Puccinia triticina f. sp. tritici) in Einkorn (Triticum monococcum) by massive analysis of cDNA ends (MACE)
Albrecht Serfling1,2, Sven Templer1,3, Dragan Perovic1, Frank Ordon1

Triticum monococcum, a valuable source for horizontal resistance against P. triticina was analyzed microscopically and by transcriptional profiling. MACE showed the increased expression of chitinases, kinases, peroxidases and pathogenesis related genes in the first 8 hai. The high number of differentially expressd tags and the knowledge about SNPs facilitates in silico mapping and the development of polymorphic markers which may accelerate the transfer of this prehaustorial resistance

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Genetic progress in the Romanian triticale breeding program
Ittu Gheorghe*, Saulescu Nicolae, Ittu Mariana, Mustatea Pompiliu and Marinciu Cristina

Since 1984 yield raised up to 43 kg / ha¹ and year¹ or 0.74 % /year¹ this progress being achieved mainly by improving fertility of spikes, plumpness of kernel, the test weight and introduction of short straw RhtB1b (Rht1) and Ddw1 (Hl) genes. In order to improve the yield stability, under predictions of global climatic changes, improvement of genetic diversity to powdery mildew, leaf and yellow rusts, virus and spike diseases and also for pre-harvest sprouting are still demanded.

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Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.

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Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.

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A non-destructive method to assess changes in the biomass of small-grain cereals under field conditions
D. Villegas1, J. Lozano2 and C. Royo1

The poster describes a non-destructive method to assess biomass based on remote sensing techniques. This method is suitable for field phenotyping and it is useful to assess biomass evolution as well as to estimate radiation use efficiency.

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Genetic integrity of rice seeds during ageing and gene bank storage by SSR analysis
I. O. Daniel, K. A. Adeboye, O. W. Adabale, E. S. Aladele and A. Boerner

Rice seeds loose germination capacity with ageing. Loses in genetic integrity was found to occur during artificial ageing at a time when percentage germination declined to 54%.

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Scientific News
Researchers Develop Detailed Genetic Map of World Wheat Varieties
Kansas State University researchers are releasing a study that details the first haplotype map of wheat.
New Test To Revolutionise Disease Detection In People, Crops And Stock
A single-drop DNA test invented by UQ scientists could revolutionise the detection of diseases in humans, livestock and crops.
Fast-Growing Trees That Are Easier To Turn Into Fuel
Researchers at the University of Georgia have discovered that manipulation of a specific gene in a hardwood tree species not only makes it easier to break down the wood into fuel, but also significantly increases tree growth.
Study Seeks to Reduce Effects of the South’s Most Costly Weed
A new study published in the journal Weed Science.
CT Scanning Shows Why Tilting Trees Produce Better Biofuel
Researchers use X-ray micro-computed tomography to examine the willow's growth through high resolution 3D images.
Scientists Transfer Pathogen-Sensing ‘Antenna’ Gene To Wheat
A receptor that recognises bacteria from Arabidopsis thaliana has been transferred to wheat.
Novel Online Bioinformatics Tool Significantly Reduces Time Of Multiple Genome Analysis
UK research collaboration develops a new bioinformatics pipeline that enables automated primer design for multiple genome species, significantly reducing turnaround time.
Biofuel Proteomics
Joint BioEnergy Institute Researchers use proteomics to profile switchgrass.
Parasite Provides Clues To Evolution Of Plant Diseases
A new study into the generalist parasite Albugo candida, cause of white rust of brassicas, has revealed key insights into the evolution of plant diseases to aid agriculture and global food security.
Fighting the Colorado Potato Beetle with RNA
RNA interference protects potato plants against herbivore attack.
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