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Genomics Research

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Defining off-target cleavage in a pair of Zinc Finger Nucleases

Poster

Published: April 13, 2012

Defining off-target cleavage in a pair of Zinc Finger Nucleases content piece image

Studies on Zinc Finger Nucleases (ZFN) have shown that they can be toxic in organisms. This is potentially due to ZFN cleavage at multiple off-target sites. In applications of ZFNs in human gene therapy, this off-target cleavage is intolerable. We are attempting to develop a procedure to identify these off-target sites in Drosophila. We can then analyze every new ZFN pair for potential off-target cleavage and select and redesign it to work more efficiently.