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FastStart PCR Master

Product Description
Ready-to-use, double-concentrated one-component Hot Start master mix for amplification of DNA-fragments up to 2 kb by polymerase chain reaction (PCR).

FastStart PCR Master can be used instead of single reagents for nearly all hot start PCR applications. FastStart PCR Master allows you to immediately set up a PCR without time-consuming thawing and pipetting steps. This minimizes the risk of contamination and saves you valuable time for your research.

The FastStart PCR Master generates PCR products with 3'-single A overhangs; therefore, T/A cloning can be applied. Cloning in blunt-end vectors requires a polishing step in advance. FastStart PCR Master accepts modified nucleotides such as DIG-dUTP. Labeling with DIG-dUTP can be achieved by adding the modified nucleotide in a final concentration of 10 µM to the final volume.

Combine this master mix with our Transcriptor First Strand cDNA Synthesis Kit to achieve superior results in two-step RT-PCR.
Product FastStart PCR Master
Company Roche - Products Listing
Price Request a quote
More Information View company product page
Catalog Number 04710452001
Quantity 1 kit 10 x 5 ml for up to 5,000 reactions of 20 µl final reaction volume
Company Logo

Roche - Products Listing
Roche Diagnostics Corporation Roche Applied Science P.O. Box 50414 9115 Hague Road Indianapolis, IN 46250-0414 US

Tel: +1-800-428-5433
Fax: +1-800-428-2883

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