For isolation of total RNA from plant tissues, optimized for use with bead based homogenizers such as the PowerLyzer™ 24.
Features • Rapid isolation of total RNA from plant tissues in only 35 minutes • Optimized for use with bead based homogenizers such as the PowerLyzer™ 24 • Yields high quality RNA ready for qPCR • Safe and easy protocol uses NO phenol/chloroform extraction, CsCl gradients or ethanol precipitation • Includes all ready-to-use RNase-free reagents and all required spin filters and tubes
The PowerLyzer™UltraClean® Plant RNA Isolation Kit is designed to isolate total RNA from a wide variety of plant materials. Sample sizes from 5 to 100 mg can be processed. All contaminants, such as polysaccharides, are removed, and the eluted RNA is ready for all downstream applications.
Fresh or frozen plant tissues are homogenized using a bead based homogenizer like the PowerLyzer™ 24. After cell lysis, the released RNA is captured on a silica membrane spin filter. The RNA bound to the filter is washed to remove all contaminants. Finally, the RNA is recovered in certified RNase-free water (provided). The eluted RNA is very pure and ready for sensitive applications such as reverse-transcription PCR.