Rainin Instrument, a METTLER-TOLEDO subsidiary, has published a new white paper entitled “High Throughput FPLC with Packed Resin Tip Technology.”
This addition to METTLER-TOLEDO’s thought-leadership library helps shed light on how novel PureSpeed™ resin tips promote easier, faster and more cost-effective protein and antibody purification than can be achieved using traditional gravity/spin columns.
Using conventional spin columns, protein purification requires a relatively large amount of resin for acceptable performance.
However, experiments detailed in the white paper indicate that the higher resin volumes required in spin columns are not the key to optimizing workflow.
PureSpeed’s bidirectional flow was shown to load PureSpeed’s relatively small resin bed with the most protein possible, providing superior final concentrations and purity when compared to the spin column procedure.
Protein capture was shown to be more repeatable as well, even at significantly reduced elution volumes.
The improved concentrations that PureSpeed enables help provide samples robust enough for assaying or downstream processing. Generally, this saves time by eliminating the need to concentrate recovered protein in a separate step.
The paper also offers guidelines for using PureSpeed tip, body and bed sizes to gain maximum protein/antibody processing.
For a complete copy of the paper, or for more on how PureSpeed can help streamline protein purification saving time and money in the process, visit www.mt.com/purespeed.