Corporate Banner
Satellite Banner
Biologics & Bioprocessing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

White Paper Explores Effective Protein Purification

Published: Friday, October 12, 2012
Last Updated: Thursday, October 11, 2012
Bookmark and Share
New white paper entitle “High Throughput FPLC with Packed Resin Tip Technology.”

Rainin Instrument, a METTLER-TOLEDO subsidiary, has published a new white paper entitled “High Throughput FPLC with Packed Resin Tip Technology.”

This addition to METTLER-TOLEDO’s thought-leadership library helps shed light on how novel PureSpeed™ resin tips promote easier, faster and more cost-effective protein and antibody purification than can be achieved using traditional gravity/spin columns.

Using conventional spin columns, protein purification requires a relatively large amount of resin for acceptable performance.

However, experiments detailed in the white paper indicate that the higher resin volumes required in spin columns are not the key to optimizing workflow.

PureSpeed’s bidirectional flow was shown to load PureSpeed’s relatively small resin bed with the most protein possible, providing superior final concentrations and purity when compared to the spin column procedure.

Protein capture was shown to be more repeatable as well, even at significantly reduced elution volumes.

The improved concentrations that PureSpeed enables help provide samples robust enough for assaying or downstream processing. Generally, this saves time by eliminating the need to concentrate recovered protein in a separate step.

The paper also offers guidelines for using PureSpeed tip, body and bed sizes to gain maximum protein/antibody processing.

For a complete copy of the paper, or for more on how PureSpeed can help streamline protein purification saving time and money in the process, visit www.mt.com/purespeed.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

13 Ways to Stop an Unseen Force from Disrupting Weighing
Download a free Mettler Toledo paper to discover how to halt static’s negative effects before the next weigh-in.
Saturday, October 03, 2015
Pharma Tech Trends and Development Cost Solutions
METTLER TOLEDO’s new on-demand webinar, “Methods to Improve Chemical Process Development,” takes on the challenges of today’s pharmaceutical market.
Thursday, April 04, 2013
Scientific News
Understanding Female HIV Transmission
Glowing virus maps points of entry through entire female reproductive tract for first time.
Soy Shows Promise as Natural Anti-Microbial Agent
Soy isoflavones and peptides may inhibit the growth of microbial pathogens that cause food-borne illnesses, according to a new study from University of Guelph researchers.
Designing Better Drugs
A rational drug engineering approach could breathe new life into drug development.
AstraZeneca to Sequence 2 Million Genomes in Search for New Drugs
Company launches integrated genomics approach which aims to transform drug discovery and development.
Factors Influencing Influenza Vaccine Effectiveness Uncovered
The long-held approach to predicting seasonal influenza vaccine effectiveness may need to be revisited, new research suggests.
Study Finds Factors That May Influence Influenza Vaccine Effectiveness
Researchers at NIH have suggested that the long-held approach to predicting seasonal influenza vaccine effectiveness may need to be revisited.
New Model to Enhance Zika Virus Research
The model will allow researchers to better understand how the virus causes disease and aid in the development of antiviral compounds and vaccines.
Improving Flu Vaccine Effectiveness
NIH study finds factors that may influence influenza vaccine effectiveness.
BMS’s Opdivo Clinical Trial Shows Promise
Safety profile of the combination regimen from CheckMate -069 was consistent with previously reported studies and adverse events were managed using established safety algorithms.
CNS Inflammation: A Pathway and Possible Drug Target
Scientists have long known that the central nervous system (CNS) has a remarkable ability to limit excessive inflammation in the presence of antigens or injury, but how it works has been unclear.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!