Corporate Banner
Satellite Banner
Biologics & Bioprocessing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Advanced Pipetting Technology Helps Optimize Protein Purification Processes

Published: Monday, December 03, 2012
Last Updated: Monday, December 03, 2012
Bookmark and Share
New white paper highlights the use of Rainin PureSpeed™ protein tips.

Rainin Instrument, a Mettler Toledo subsidiary, has published a new white paper describing the optimization of protein/antibody purification.

The paper, entitled “Optimizing Protein Purification Conditions,” highlights the use of Rainin PureSpeed™ Protein Tips.

Using Rainin’s E4 XLS electronic pipette, PureSpeed tips allow for bidirectional sample flow over the purification resin bed, maximizing target protein capture by allowing the binding reaction to reach equilibrium.

Potential concentration steps, which can actually degrade protein quality, are eliminated, saving time and costly rework.

To demonstrate the process, a 6-His-tagged protein and s 20 µL Ni-IMAC PureSpeed tips were used with a Rainin E4 XLS pipette. Back-and-forth capture flow was used to ensure complete resin exposure during all steps.

Concentrations of imidazole in the wash buffer and final elution were varied to help illustrate purification optimization, targeting both quantity and purity of captured protein.

The experiments illustrate that PureSpeed wash and elution chemistries, which are carried out at reduced volumes, generalize to larger scale preparative systems, making PureSpeed a cost-effective optimization tool.

The paper also reviews aspects such as protein size that can affect the capture equilibrium as well as ways to help enhance protein capture by adjusting pH and flow rate or increasing cycle numbers.

Rainin’s ColorTrak™ Guide also proved useful at helping non-expert operators load and purify a specific protein or antibody.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Pharma Tech Trends and Development Cost Solutions
METTLER TOLEDO’s new on-demand webinar, “Methods to Improve Chemical Process Development,” takes on the challenges of today’s pharmaceutical market.
Thursday, April 04, 2013
Scientific News
Less May Be More in Slowing Cholera Epidemics
Mathematical model shows more cases may be prevented and more lives saved when using one dose of cholera vaccine instead of recommended two doses.
NIH Launches Human RSV Study
Study aims to understand infection in healthy adults to aid development of RSV medicines, vaccines.
Flu Remedies Help Combat E. coli Bacteria
Physiologists from the University of Zurich have now discovered why the intestinal bacterium Escherichia coli (E. coli) multiplies heavily and has an inflammatory effect.
'Fountain of Youth' Protein Points to Possible Human Health Benefit
Patients with higher blood levels of growth factor have lower risk of cardiovascular problems.
Lemon Juice and Human Norovirus
Citric acid may prevent the highly contagious norovirus from infecting humans, scientists discovered from the German Cancer Research Center.
Study Backs Flu Vaccinations for Elderly
Brown University researchers found vaccines well matched to the year’s flu strain significantly reduce deaths and hospitalizations compared to when the match is poor, suggesting that vaccination indeed makes a difference.
Inciting an Immune Attack on Cancer Cells
A new minimally invasive vaccine that combines cancer cells and immune-enhancing factors could be used clinically to launch a destructive attack on tumors.
Protein Found to Play a Key Role in Blocking Pathogen Survival
Calprotectin fends off microbial invaders by limiting access to iron, an important nutrient.
Major Advance Toward More Effective, Long-Lasting Flu Vaccine
Collaboration shows vaccine candidate can produce powerful ‘broadly neutralizing antibodies’ in animal models.
Immune System: Help for Killer Cells
A study from the University of Bonn may show the way to more effective vaccines.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!