Corporate Banner
Satellite Banner
Biologics & Bioprocessing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Advanced Pipetting Technology Helps Optimize Protein Purification Processes

Published: Monday, December 03, 2012
Last Updated: Monday, December 03, 2012
Bookmark and Share
New white paper highlights the use of Rainin PureSpeed™ protein tips.

Rainin Instrument, a Mettler Toledo subsidiary, has published a new white paper describing the optimization of protein/antibody purification.

The paper, entitled “Optimizing Protein Purification Conditions,” highlights the use of Rainin PureSpeed™ Protein Tips.

Using Rainin’s E4 XLS electronic pipette, PureSpeed tips allow for bidirectional sample flow over the purification resin bed, maximizing target protein capture by allowing the binding reaction to reach equilibrium.

Potential concentration steps, which can actually degrade protein quality, are eliminated, saving time and costly rework.

To demonstrate the process, a 6-His-tagged protein and s 20 µL Ni-IMAC PureSpeed tips were used with a Rainin E4 XLS pipette. Back-and-forth capture flow was used to ensure complete resin exposure during all steps.

Concentrations of imidazole in the wash buffer and final elution were varied to help illustrate purification optimization, targeting both quantity and purity of captured protein.

The experiments illustrate that PureSpeed wash and elution chemistries, which are carried out at reduced volumes, generalize to larger scale preparative systems, making PureSpeed a cost-effective optimization tool.

The paper also reviews aspects such as protein size that can affect the capture equilibrium as well as ways to help enhance protein capture by adjusting pH and flow rate or increasing cycle numbers.

Rainin’s ColorTrak™ Guide also proved useful at helping non-expert operators load and purify a specific protein or antibody.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,400+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

13 Ways to Stop an Unseen Force from Disrupting Weighing
Download a free Mettler Toledo paper to discover how to halt static’s negative effects before the next weigh-in.
Saturday, October 03, 2015
Pharma Tech Trends and Development Cost Solutions
METTLER TOLEDO’s new on-demand webinar, “Methods to Improve Chemical Process Development,” takes on the challenges of today’s pharmaceutical market.
Thursday, April 04, 2013
Scientific News
Understanding Female HIV Transmission
Glowing virus maps points of entry through entire female reproductive tract for first time.
Soy Shows Promise as Natural Anti-Microbial Agent
Soy isoflavones and peptides may inhibit the growth of microbial pathogens that cause food-borne illnesses, according to a new study from University of Guelph researchers.
Designing Better Drugs
A rational drug engineering approach could breathe new life into drug development.
AstraZeneca to Sequence 2 Million Genomes in Search for New Drugs
Company launches integrated genomics approach which aims to transform drug discovery and development.
Factors Influencing Influenza Vaccine Effectiveness Uncovered
The long-held approach to predicting seasonal influenza vaccine effectiveness may need to be revisited, new research suggests.
Study Finds Factors That May Influence Influenza Vaccine Effectiveness
Researchers at NIH have suggested that the long-held approach to predicting seasonal influenza vaccine effectiveness may need to be revisited.
New Model to Enhance Zika Virus Research
The model will allow researchers to better understand how the virus causes disease and aid in the development of antiviral compounds and vaccines.
Improving Flu Vaccine Effectiveness
NIH study finds factors that may influence influenza vaccine effectiveness.
BMS’s Opdivo Clinical Trial Shows Promise
Safety profile of the combination regimen from CheckMate -069 was consistent with previously reported studies and adverse events were managed using established safety algorithms.
CNS Inflammation: A Pathway and Possible Drug Target
Scientists have long known that the central nervous system (CNS) has a remarkable ability to limit excessive inflammation in the presence of antigens or injury, but how it works has been unclear.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!