Corporate Banner
Satellite Banner
Biologics & Bioprocessing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Rockland Immunochemicals Releases Host Cell Protein Antibodies

Published: Tuesday, January 29, 2013
Last Updated: Tuesday, January 29, 2013
Bookmark and Share
New host cell antibodies and custom assays improve bioprocessing workflow by detecting host cell protein contaminants.

Rockland Immunochemicals, Inc. has announced the release of Host Cell Protein (HCP) antibodies that detect high, medium and low molecular weight proteins.  Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP antibodies are used to detect impurities in biologically-based therapeutics. Assays are further developed using custom-manufactured process specific antibodies.

Rockland’s new HCP antibodies will be released and a standardized HCP immunoassay development process presented during the Well Characterized Biotechnology Pharmaceuticals (WCBP) Symposium in Washington, DC January 29-31. This United States Food and Drug Administration (FDA) co-sponsored symposium is the pre-eminent conference addressing the analytical development/regulatory control interface for biotechnology derived health intervention products.

The FDA requires that pharmaceutical and biotechnology companies producing therapeutic antibodies minimize HCP contamination to an acceptable level. Failure to identify and sufficiently remove contaminants early in drug development can result in reduced drug efficacy or adverse toxic or immunological reactions in patients.

ELISA, the most widely used assay for determining the presence of HCPs, uses a polyclonal antibody mixture raised against host cell proteins. The accepted workflow for evaluating the immunocoverage of HCP antibodies involves 2-D gel electrophoresis followed by Western blotting. Rockland now provides Bioprocessing researchers with antibodies and custom HCP assay development  services that streamlines and regulates this workflow.

At the WCBP Symposium, Rockland researchers will present data that shows an improved standardized immunoassay development process for HCP detection using 2D electrophoresis and western blotting. In collaboration with Bio-Rad, the PROTEAN® i12™ IEF System was used to develop the HCP antibodies and measure/calibrate assays. Detecting impurities often unseen by 1-D electrophoresis, this system delivers more complete insight into the immunocoverage of protein components of the host cell lysate. The newly developed Rockland antibodies have been fully evaluated using the 2-D electrophoresis workflow and  provide efficient immunocoverage of E.coli HCP.

“Rockland’s expertise in HCP antibodies and HCP assay development services has allowed Bio-Rad to affirm the value of optimized IEF separations in generating a robust 2D-electrophoresis based assay for evaluating anti-HCP antibodies, ” said Sricharan Bandhakavi, Senior Scientist at Bio-Rad Laboratories.

"Over the last decade, BioPharma has invested significant amounts of money to develop large molecule (antibody) drugs. As these drugs move forward in the approval process, reagents and assays used to analyze purity will increase in importance,” commented Jim Fendrick, President and CEO of Rockland Immunochemicals. “With our well-developed antibody and assay technology, Rockland is well positioned to take advantage of this opportunity and set the standard for optimal quality of application."


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Immunotherapy Agent Benefits Patients with Drug-Resistant Multiple Myeloma in First Human Trial
Daratumumab proved generally safe in patients, even at the highest doses.
Inciting an Immune Attack On Cancer Cells
A new minimally invasive vaccine that combines cancer cells and immune-enhancing factors could be used clinically to launch a destructive attack on tumors.
Less May Be More in Slowing Cholera Epidemics
Mathematical model shows more cases may be prevented and more lives saved when using one dose of cholera vaccine instead of recommended two doses.
NIH Launches Human RSV Study
Study aims to understand infection in healthy adults to aid development of RSV medicines, vaccines.
Flu Remedies Help Combat E. coli Bacteria
Physiologists from the University of Zurich have now discovered why the intestinal bacterium Escherichia coli (E. coli) multiplies heavily and has an inflammatory effect.
'Fountain of Youth' Protein Points to Possible Human Health Benefit
Patients with higher blood levels of growth factor have lower risk of cardiovascular problems.
Lemon Juice and Human Norovirus
Citric acid may prevent the highly contagious norovirus from infecting humans, scientists discovered from the German Cancer Research Center.
Study Backs Flu Vaccinations for Elderly
Brown University researchers found vaccines well matched to the year’s flu strain significantly reduce deaths and hospitalizations compared to when the match is poor, suggesting that vaccination indeed makes a difference.
Inciting an Immune Attack on Cancer Cells
A new minimally invasive vaccine that combines cancer cells and immune-enhancing factors could be used clinically to launch a destructive attack on tumors.
Protein Found to Play a Key Role in Blocking Pathogen Survival
Calprotectin fends off microbial invaders by limiting access to iron, an important nutrient.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!