Corporate Banner
Satellite Banner
Biologics & Bioprocessing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Protein Glycosylation Evaluation in Less Than 10 µg of Protein Samples

Published: Friday, August 23, 2013
Last Updated: Friday, August 23, 2013
Bookmark and Share
Evaluating protein glycosylation in limited-quantity samples by HPAE-PAD.

Thermo Fisher Scientific has developed a new approach that combines high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) analysis, acid hydrolysis, and enzymatic digestion of protein glycans to determine information about glycan identity and terminal carbohydrate linkage isomers from small amounts of protein-just 0.5–1.6 μg per injection.

Application Note (AN) 1050: Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD demonstrates that a suite of HPAE-PAD analyses can be performed with sample quantities as low as 3-10 μg. This direct determination approach saves time and reagent costs.

Cancer research frequently involves studying changes in protein glycosylation to identify important biomarkers such as prostate-specific antigen (PSA).

The AN 1050 methodology uses an ion chromatography system configured for HPAE-PAD to investigate human PSA N-linked glycosylation and to evaluate the potential presence of O-linked glycans with methods optimized to work with small quantities of glycoproteins.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More than 4,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Thermo Strengthens Bioprocessing Offering with Advanced Scientifics Acquisition
Thermo Fisher Scientific has acquired Advanced Scientifics, Inc., a global provider of single-use technologies for customized bioprocessing solutions, for $300 million in cash.
Friday, February 06, 2015
Thermo Fisher Q3 Revenues up 31 Percent
Thermo Fisher announced that revenues grew 31% to $4.17 billion for the third quarter ended September 27, 2014.
Thursday, October 23, 2014
Thermo Fisher, BioAnalytix Collaborate
Collaboration to develop advanced biopharmaceutical analysis workflows.
Thursday, June 12, 2014
Thermo Reports 22% Revenue Rise in Q1 2014
Results include the addition of Life Technologies as of February 4, 2014, and exclude the previously announced related divestitures as of March 22, 2014.
Wednesday, April 23, 2014
Thermo Completes Life Tech Acquisition
The completion of the transaction follows the receipt of all required regulatory approvals.
Tuesday, February 04, 2014
Biosimilar Characterization R&D Facility Opens in Austria
University of Salzburg, Sandoz and Thermo Fisher Scientific collaborate to advance protein-based pharmaceutical development, production, QA/QC.
Wednesday, October 23, 2013
Thermo Fisher Scientific Opens Its First Dry Powder Media Facility in APAC Region
Singapore facility delivers manufacturing redundancy and increases supply assurance for global biologic drug discovery and production.
Tuesday, August 06, 2013
Thermo Fisher Scientific Expands Shanghai Production Capacity
First global company to locally produce cell culture consumable products in China for China.
Friday, January 21, 2011
Scientific News
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Designing Potential AIDS Vaccine Candidates
Findings represent ‘big accomplishment’ in biomedical engineering and design.
Anthrax Proteins Might Help Treat Cancerous Tumors
Studies in mice reveal novel treatment regimen.
HIV Structure Stabilized
Findings represent ‘big accomplishment’ in biomedical engineering and design.
Anthrax Vaccine Protects Monkeys
Vaccination with the anthrax capsule—a naturally occurring component of the bacterium that causes the disease—completely protected monkeys from lethal anthrax infection, according to a recently published study.
Long-Term Culturing of Adult Stem Cells
A new procedure developed by Harvard Stem Cell Institute researchers (HSCI) at Massachusetts General Hospital (MGH) may revolutionize the culturing of adult stem cells.
Solutions for Biotherapeutic Characterization
Innovation to speed the routine.
Plant-Based Vaccine Among Front Runners In Search For New Polio Jab
A researcher from Norwich is part of a consortium that has been awarded $1.5 million to develop safer polio vaccines, using a new technique developed at the John Innes Centre.
Harnessing Helpful Microbes
Seeking to further harness microbes’ many uses, the federal government has launched the National Microbiome Initiative (NMI) to “foster the integrated study of microbiomes across different ecosystems.”
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!