Corporate Banner
Satellite Banner
Biologics & Bioprocessing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Method for Microcarrier Cell Culture using TAP’s ambr Micro Bioreactors

Published: Thursday, October 31, 2013
Last Updated: Thursday, October 31, 2013
Bookmark and Share
Saves time optimizing process conditions enabling rapid vaccine production.

TAP Biosystems has announced that it has developed a new method of using microcarriers with its ambr™ micro bioreactor system.

This technique enables scientists to produce uniform microcarrier samples and perform automated media exchange to rapidly optimize parameters for culturing adherent cell lines on microcarriers, thus shortening timelines for successfully scaling up manufacturing of vaccines and cell therapies.

Scientists at TAP have utilized the ambr workstation to develop an automated method for providing highly consistent dispensing of microcarriers to multiple 10-15mL ambr microbioreactors.

This means scientists can then rapidly test up to 24 cell-specific culture parameters in parallel including stirring, media formulation or feed strategies to determine the optimum conditions for cell attachment, growth rate and vaccine titre, for example.

The new method, which has been developed using Vero adherent cells attached to Cytodex® 1 microcarriers, allows a 20% media exchange to be performed at any time of the day or night on 24 ambr vessels in approximately 4h.

Since the method allows even microcarrier distribution, scientists can then study up to 24 different parameters in parallel, simultaneously.

This reduces reliance on spinner flasks and bench top bioreactors, saving vaccine and cell therapy manufacturers many months of scale-up process development work.

The method and results of the study are available in a technical application note, which can be requested via the link: http://www.tapbiosystems.com/ambr_microvacc/technoterequest_corporate.asp
Dr Barney Zoro, ambr Product Manager at TAP Biosystems stated: "There is a great deal of interest in developing vaccines using attachment dependent Vero cells and microcarriers as they allow cell propagation in bioreactors rather than roller bottles. The use of bioreactors can provide greater process control, resulting in high-titre vaccine production. However, the main issue is how to accurately mimic bioreactor conditions as producing evenly distributed microcarriers and performing media exchange can be problematic using spinner flasks and benchtop bioreactors. Our new method for microcarrier culture using the ambr system means scientists can begin their experiments with uniform microcarrier samples and perform media exchange without interruption of stirring.”

Zoro added: “Being able to assess different parameters, as well as perform process development in weeks rather than months when using ambr will save scientists valuable time, enabling them to rapidly scale up an optimum cell culture process for more affordable vaccines or as a quick response to unexpected situations such as a pandemic threat.”


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Merck and TAP Biosystems Finalists for “Best Collaboration Award”
Recognizing ambr250 as a breakthrough technology for process development.
Wednesday, October 08, 2014
TAP and Gallus Co-host Free Webinar
Explaining the benefits of using ambr15 microbioreactors for DoE.
Wednesday, October 01, 2014
New Webinars Explore Feed Strategy Challenges Using Mini Bioreactors
Detailing how to achieve consistent feeding regimes for reproducible scale-up.
Thursday, September 25, 2014
New Webinar on Mimicking Perfusion Culture Using Micro Bioreactors
Presents validation data for improving media screening and process optimization.
Friday, February 14, 2014
Scientific News
First Artificial Ribosome Designed
Researchers at the University of Illinois at Chicago and Northwestern University have engineered a tethered ribosome that works nearly as well as the authentic cellular component, or organelle, that produces all the proteins and enzymes within the cell.
Experimental MERS Vaccine Shows Promise in Animal Studies
A two-step regimen of experimental vaccines against Middle East respiratory syndrome (MERS) prompted immune responses in mice and rhesus macaques, report National Institutes of Health scientists who designed the vaccines.
Sweet Revenge Against Superbugs
A special type of synthetic sugar could be the latest weapon in the fight against superbugs.
Researchers Develop Vaccine that Protects Primates Against Ebola
A collaborative team from The University of Texas Medical Branch at Galveston and the National Institutes of Health have developed an inhalable vaccine that protects primates against Ebola.
Universal Flu Vaccine in the Works
A new study has demonstrated a potential strategy for developing a flu vaccine with potent, broad protection.
Immunotherapy Shows Promise for Myeloma
A strategy, which uses patients’ own immune cells, genetically engineered to target tumors, has shown significant success against multiple myeloma, a cancer of the plasma cells that is largely incurable.
Ferring Bets on Bacteriophages to Treat Inflammatory Bowel Disease
Ferring Pharmaceuticals have annoucned that it will collaborate with Intralytix in the latest phase of its early stage development programme for a bacteriophage-based therapy for inflammatory bowel disease (IBD).
A Novel Drug to FIght Malaria
An international team of scientists has announced that a new compound to fight malaria is ready for human trials.
Ebola Vaccine Trial Begins in Senegal
A clinical trial to evaluate an Ebola vaccine has begun in Dakar, Senegal, after initial research started at the Jenner Institute, Oxford University.
New Cell Structure Finding Might Lead to Novel Cancer Therapies
University of Warwick scientists in the U.K. say they have discovered a cell structure which could help researchers understand why some cancers develop.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!