Corporate Banner
Satellite Banner
Biologics & Bioprocessing
Scientific Community
 
Become a Member | Sign in
Home>Resources>White Papers>This White Paper
  White Papers
Scientific News
Developing a More Precise Seasonal Flu Vaccine
During the 2014-15 flu season, the poor match between the virus used to make the world’s vaccine stocks and the circulating seasonal virus yielded a vaccine that was less than 20 percent effective.
Fighting Cancer with Borrowed Immunity
A new step in cancer immunotherapy: researchers from the Netherlands Cancer Institute and University of Oslo/Oslo University Hospital show that even if one's own immune cells cannot recognize and fight their tumors, someone else's immune cells might.
Modified Microalgae Converts Sunlight into Valuable Medicine
A special type of microalgae can soon produce valuable chemicals such as cancer treatment drugs and much more just by harnessing energy from the sun.
Immune Cells Remember Their First Meal
Scientists at the University of Bristol have identified the trigger for immune cells' inflammatory response – a discovery that may pave the way for new treatments for many human diseases.
Paper Filter Can Remove Viruses from Water
A new paper filter can purify water from viruses, even the most difficult and contagious.
Large-scale HIV Vaccine Trial to Launch in South Africa
NIH-funded study will test safety, efficacy of vaccine regimen.
New HIV Vaccine Target Discovered
NIH-Led team have discovered a new vaccine target site on HIV.
Mimicking Evolution to Create Novel Proteins
A study by researchers in the Kuhlman lab offers a new route to design the 'cellular machines' needed to understand and battle diseases.
Antibody Therapy Opens Door to Potential New Treatment for HIV
Researchers at Rockefeller University show how a broadly neutralizing antibody could be used to help fight HIV.
Investigational Malaria Vaccine Protects Healthy U.S. Adults
Researchers at NIH have found that the malaria vaccine protected a small number of healthy, malaria-naïve adults in the U.S. from infection for more than one year after immunization.
Scroll Up
Scroll Down

Sensitivity of a combined DLS/Raman technique to estimate the conformational and colloidal stability of protein therapeutics
Bookmark and Share

Malvern Instruments Limited

Combining dynamic light scattering (DLS) and Raman spectroscopy provides the ability to extract a wealth of chemical, structural, and physical parameters from proteins at both low and formulation concentrations. DLS, used to study protein size, and Raman, used to determine protein conformation, is combined into a single instrument to monitor stressed protein samples under identical conditions.

DLS is ideally suited to measuring the size of proteins at low concentrations (down to 0.1 mg/mL), while Raman spectroscopy is ideally suited to deriving secondary and tertiary structural markers from higher concentration protein samples (50 mg/mL or greater). One practical area of interest is understanding the lowest concentration of a protein that can be studied using these combined analytical techniques. Because DLS is known to be appropriate for analyzing low concentration samples, this note will outline the lowest practical protein concentration limit for acquiring Raman spectra, and determine if the spectra provide useful secondary and tertiary structure information. Results using lysozyme indicate that useful information from concentrations as low as 3 mg/mL can be acquired, while monoclonal antibody samples (mAbs) may require a higher concentration to obtain correct structural and thermal information.

The ability of a combined DLS/Raman instrument to study various concentrations of protein samples is explored. DLS, with its capability to measure protein size, is ideal for low concentration samples, while Raman, with its ability to interrogate protein secondary and tertiary structure, is ideal for high concentration samples. Joining the techniques into a single technology allows for the determination of size and structure on a single small volume sample. However, because the complementary techniques have differing ideal concentrations, it is important to first establish the upper and lower practical concentration boundaries for a protein sample. This note focuses on determining the lowest concentration limit for studying proteins with combined DLS/ Raman. 

Further Information

Related Content

Oxford Researcher Explains Central Role of Microcalorimetry in Meningitis Vaccine Development
A highly sensitive, fully automated, high-throughput differential scanning calorimeter.
Saturday, October 24, 2015
Malvern Instruments Establishes New Biopharmaceutical Applications Laboratory
Malvern Instruments has established a new biopharmaceutical applications laboratory in the heart of San Diego’s biotechnology cluster.
Friday, July 31, 2015
Researchers use Nanoparticle Tracking Analysis to Study Extracellular Vesicles
Scientists are working to find a way deliver a specific microRNA species capable of retarding the growth of tumor cells.
Thursday, May 28, 2015
Malvern Completes Acquisition of MicroCal
Company announces purchase of Archimedes product from Affinity Biosensors.
Friday, July 25, 2014
Malvern to Acquire MicroCal Business from GE
Malvern Instruments has entered into an agreement with GE Healthcare Life Sciences to acquire the company’s MicroCal™ microcalorimetry business.
Wednesday, June 04, 2014
Regional Growth Fund Success Sees Malvern Instruments Stepping up UK Recruitment
£3 million support from the UK Government’s Regional Growth Fund (RGF) will lead to the creation of 47 new jobs in the UK over the next five years.
Tuesday, May 20, 2014
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!