Corporate Banner
Satellite Banner
Biologics & Bioprocessing
Scientific Community
 
Become a Member | Sign in
Home>Resources>White Papers>This White Paper
  White Papers
Scientific News
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Anthrax Proteins Might Help Treat Cancerous Tumors
Studies in mice reveal novel treatment regimen.
HIV Structure Stabilized
Findings represent ‘big accomplishment’ in biomedical engineering and design.
Anthrax Vaccine Protects Monkeys
Vaccination with the anthrax capsule—a naturally occurring component of the bacterium that causes the disease—completely protected monkeys from lethal anthrax infection, according to a recently published study.
Long-Term Culturing of Adult Stem Cells
A new procedure developed by Harvard Stem Cell Institute researchers (HSCI) at Massachusetts General Hospital (MGH) may revolutionize the culturing of adult stem cells.
Solutions for Biotherapeutic Characterization
Innovation to speed the routine.
Plant-Based Vaccine Among Front Runners In Search For New Polio Jab
A researcher from Norwich is part of a consortium that has been awarded $1.5 million to develop safer polio vaccines, using a new technique developed at the John Innes Centre.
Harnessing Helpful Microbes
Seeking to further harness microbes’ many uses, the federal government has launched the National Microbiome Initiative (NMI) to “foster the integrated study of microbiomes across different ecosystems.”
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
A New Approach to Chemical Synthesis
Communesins, originally found in fungus, could hold potential as cancer drugs.
Scroll Up
Scroll Down

Sensitivity of a combined DLS/Raman technique to estimate the conformational and colloidal stability of protein therapeutics
Bookmark and Share

Malvern Instruments Limited

Combining dynamic light scattering (DLS) and Raman spectroscopy provides the ability to extract a wealth of chemical, structural, and physical parameters from proteins at both low and formulation concentrations. DLS, used to study protein size, and Raman, used to determine protein conformation, is combined into a single instrument to monitor stressed protein samples under identical conditions.

DLS is ideally suited to measuring the size of proteins at low concentrations (down to 0.1 mg/mL), while Raman spectroscopy is ideally suited to deriving secondary and tertiary structural markers from higher concentration protein samples (50 mg/mL or greater). One practical area of interest is understanding the lowest concentration of a protein that can be studied using these combined analytical techniques. Because DLS is known to be appropriate for analyzing low concentration samples, this note will outline the lowest practical protein concentration limit for acquiring Raman spectra, and determine if the spectra provide useful secondary and tertiary structure information. Results using lysozyme indicate that useful information from concentrations as low as 3 mg/mL can be acquired, while monoclonal antibody samples (mAbs) may require a higher concentration to obtain correct structural and thermal information.

The ability of a combined DLS/Raman instrument to study various concentrations of protein samples is explored. DLS, with its capability to measure protein size, is ideal for low concentration samples, while Raman, with its ability to interrogate protein secondary and tertiary structure, is ideal for high concentration samples. Joining the techniques into a single technology allows for the determination of size and structure on a single small volume sample. However, because the complementary techniques have differing ideal concentrations, it is important to first establish the upper and lower practical concentration boundaries for a protein sample. This note focuses on determining the lowest concentration limit for studying proteins with combined DLS/ Raman. 

Further Information

Related Content

Oxford Researcher Explains Central Role of Microcalorimetry in Meningitis Vaccine Development
A highly sensitive, fully automated, high-throughput differential scanning calorimeter.
Saturday, October 24, 2015
Malvern Instruments Establishes New Biopharmaceutical Applications Laboratory
Malvern Instruments has established a new biopharmaceutical applications laboratory in the heart of San Diego’s biotechnology cluster.
Friday, July 31, 2015
Researchers use Nanoparticle Tracking Analysis to Study Extracellular Vesicles
Scientists are working to find a way deliver a specific microRNA species capable of retarding the growth of tumor cells.
Thursday, May 28, 2015
Malvern Completes Acquisition of MicroCal
Company announces purchase of Archimedes product from Affinity Biosensors.
Friday, July 25, 2014
Malvern to Acquire MicroCal Business from GE
Malvern Instruments has entered into an agreement with GE Healthcare Life Sciences to acquire the company’s MicroCal™ microcalorimetry business.
Wednesday, June 04, 2014
Regional Growth Fund Success Sees Malvern Instruments Stepping up UK Recruitment
£3 million support from the UK Government’s Regional Growth Fund (RGF) will lead to the creation of 47 new jobs in the UK over the next five years.
Tuesday, May 20, 2014
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!