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Videos
Importance of Culturing Cells in Hypoxia...
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Featured Project3D Human Liver Tissue Testing Services
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Webcasts
Novel Technology Approaches for Capture and Characterization of Circulating Tumor CellsNovel Technology Approaches for Capture and Characterization of Circulating Tumor Cells
Henry Lin,Graduate Student, University of California, speaking at CellTech 2013.
Selection of Label-free Cellular Imaging Applications for their Integration in the Screening StrategySelection of Label-free Cellular Imaging Applications for their Integration in the Screening Strategy
Gerardo Turcatti, Ecole Polytechnique Federale De Lausanne, speaking at Screening Europe 2015.

Market Reports
3D Spheroid Culture Trends 2015
HTStec Limited

This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on three dimensional (3D) spheroid culture carried out in May 2015.
Immunoassay Auto Trends 2014
HTStec

This report comprehensively documents current practices and preferences in immunoassays and to understand future user requirements, particularly with respect to immunoassay automation.
Cell-Based Label-Free Detection Trends 2014
HTStec Limited

This market report summarizes the results of HTStec’s 4th global web-based benchmarking survey on cell-based label-free (LF) detection carried out in July and August 2014. The study also examined in detail the current market landscape, application areas and interest in purchasing new products/systems.
Biologics
RI Technologies

This report on Biologics gives a market insight into products types including Monoclonal Antibodies, Blood & Blood Products, Vaccines, Insulin and Other, for both human and animal consumption.
Multi-Mode Microplate Reader Trends 2013
HTStec

This market report summarizes the results of HTStec’s 4th industry-wide global web-based benchmarking survey on multi-mode microplate readers carried out in September 2013.
3D Cell Culture Trends 2013
HTStec

This market report summarizes the results of HTStec’s 3rd industry-wide global web-based benchmarking survey on three dimensional (3D) cell culture technologies carried out in July 2013.
Cell Culture - Rising Demand for Biologics and Growing Visibility of Approval Pathway for Biosimilars will Create Growth Opportunities
Global Data

The report analyzes the role of biopharmaceutical products that basically provide enhancement to media, sera, reagents and cell culture market. Major manufacturing technologies involved in the cell culture technology have been detailed in the report. Furthermore, the report provides competitive benchmarking for the leading players that shape the global media, sera, reagent and overall cell culture market.
Cell Migration Assays Trends
HTStec

This market report summarizes the results of HTStec’s global web-based benchmarking survey on cell migration, motility or invasion assays carried out in July 2012.
Scientific News
The Mending Tissue - Cellular Instructions for Tissue Repair
NUS-led collaborative study identifies universal mechanism that explains how tissue shape regulates physiological processes such as wound healing and embryo development.
Most Complete Human Brain Model to Date is a ‘Brain Changer’
Once licensed, model likely to accelerate study of Alzheimer’s, autism, more.
Capturing Cell Growth in 3-D
Spinout’s microfluidics device better models how cancer and other cells interact in the body.
Protein That Turns Moles Into Melanoma Cancer Identified
Moles can turn into cancer, if the genetic factors recently identified by a team of researchers at the University of Pennsylvania were not present in humans.
Scientists Grow Human Serotonin Neurons in Petri Dish
The advance could facilitate the discovery of new antidepressants and drugs for illnesses involving serotonin.
Study Details Powerful Molecular Promoter of Colon Cancers
Findings show how suppression of microRNA family of molecules leads to intestinal tumors.
From Pluripotency to Totipotency
Studies results provide new elements for the understanding of pluripotency and could increase the efficiency of reprogramming somatic cells to be used for applications in regenerative medicine.
Cancer Treatment Models get Real
Researchers at Rice Univ. and Univ. of Texas MD Anderson Cancer Center have developed a way to mimic the conditions under which cancer tumors grow in bones.
Potential Treatment for Muscular Dystrophy
A new method for producing muscle cells could offer a better model for studying muscle diseases, such as muscular dystrophy, and for testing potential treatment options.
Protein Related to Long Term Traumatic Brain Injury Complications Discovered
NIH-study shows protein found at higher levels in military members who have suffered multiple TBIs.
Business News
Sartorius Stedim Biotech and GE Healthcare Sign Worldwide OEM Supply Agreement
Expands GE’s start-to-finish offering for cost efficient manufacture of biopharmaceuticals.
ZKT gGmbH Awarded AMG Manufacturing Licence for Platelet Lysate
Turbo-drive for stem cell cultivation.
InSphero Expands Global Availability of its Patented 3D Cell Culture Platforms
PerkinElmer named as exclusive distributor of InSphero 3D culture technology.
BioCision to Develop Controlled-Rate Cell Freezing Systems for Cook Regentec Cell Therapy Vials
BioCision and Cook ink exclusive deal to combine technologies to standardize cell cryopreservation in closed-system vials.
BioTek Instruments Receives 5x5x5 Growth Award for Technology
BioTek Instruments has been recognized as one of the 5x5x5 Growth Award winners for 2015.
InSphero AG Secures CHF 20 Million Series C Financing
3D cell culture company gains new long-term investor to fuel expansion of global operations and product portfolio.
InSphero AG Secures CHF 20 Million Series C Financing
Leading 3D cell culture company gains new long-term investor to fuel expansion of global operations and product portfolio.
Thermo Fisher Agrees to Buy Alfa Aesar for $403M
The company announced a definitive agreement to buy the research chemical manufacturer for $403 million.
David Newble Joins TTP Labtech as New Managing Director
Recent appointment promises to drive innovation strategy and further strengthen R&D pipeline
Biosyntia Recieves Investment for a Bio-based Vitamin Production Platform
The company have recently received significant funding from Novo Seeds to develop a vitamin production system aimed at disrupting the current market place.

Events
3rd Annual Cell Culture Asia C
15 Sep 2015
3rd Annual Cell Culture Asia C ...
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2nd Annual Cell Culture and Bi
23 Sep 2015
2nd Annual Cell Culture and Bi ...
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EMBO | EMBL Symposium:  Seeing
06 Oct 2015
EMBO | EMBL Symposium: Seeing ...
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4th Annual Cell Culture & Biop
09 Nov 2015
4th Annual Cell Culture & Biop ...
More Information

ePosters
Increasing gene editing efficiencies in eukaryotic cell lines by selection of appropriate CRISPR-Cas9 reagents
GE Healthcare Dharmacon, Inc.

Overview of various CRISPR-Cas9 reagents to provide the highest efficiency of gene editing in your experiments.
Knockdown of p53 by Accell self-delivering siRNA causes inhibition of p53-dependent DNA damage response in IMR-32 neuroblastoma cell line and β-amyloid toxicity in rat cortical neurons
GE Healthcare Dharmacon, Inc.

Here we describe how application of Accell siRNA enabled the development of a high content screening assay in IMR-32 neuroblastoma cells and a whole culture cell viability assay in primary rat cortical neurons.
An Efficient Method for the Incorporation of Molecular Probes at Multiple/Specific sites in RNA: Levulinyl Protection for 2'-ACE ® , 5'-Silyl Oligoribonucleotide Synthesis
GE Healthcare Dharmacon, Inc.

A unique method that uses a levulinate ester as a protecting group to introduce conjugates or molecular probes to virtually any location in a synthetic RNA molecule is discussed. The Levulinyl protecting group is stable in RNA synthesis conditions and can be removed without affecting the other parts of the synthesized RNA. We show the capabilities of this approach with three high-complexity synthesis examples.
Innovative technology that enables RNAi in difficult to transfect cells
GE Healthcare Dharmacon, Inc.

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.
Innovative technology that enables RNAi in difficult to transfect cells
GE Healthcare Dharmacon, Inc.

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.
Measuring Antitumor Effect of c-Myr-Max heterodimerization inhibitor 100258-F4 on Ovarian Cancer Cells using Cellometer Imaging Cytometry
Nexcelom Bioscience LLC, University of North Carolina

The effect from the c-Mycinhibitor 100258-F4 was clearly observed from the G0/G1 cell cycle arrest and the increase in early and late apoptotic cell. The Cellometer method can measure fluorescent cell-based assays such as cell cycle, apoptosis, protein expression, autophagy and viability. The ability to export the data to FCS Express facilitates simple data analysis and reporting to generate results for publications.
Modelling CLL cell and T-cell Migration in a Dynamic Circulating Model of CLL
'Cardiff CLL Research Group, Institute of Cancer & Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff, UK. 2CRUK Institute for Cancer Studies, University of Birmingham, Vincent Driv

We have recently developed a novel circulating model of chronic lymphocytic leukaemia (CLL) that mimics the transient interactions that take place between circulating lymphocytes and vascular endothelium. Here we show that both normal and malignant lymphocytes actively underwent transendothelial migration.
Quantification of Natural Killer Cell-Mediated Cytotixicity using Celigo Imaging Cytometry
Nexcelom Bioscience LLC, The University of Texas MD Anderson Cancer Center

Time-course tracking of % lysis eliminates multiple controls & the effect of non-uniform cell seeding in the final cytotoxicity calculation. The # of cells used is less than the cells needed for Release assays & Flow Cytometry. Flow cytometry & Release assays require a seeding density of 100K target cells increasing the number of effector cells to the millions. The visual observation of ADCC or CDC on the images can be convincing to conclude the functionality of antibodies or complements.
A rapid 3D tumor spheroid analysis method using the Celigo Imaging Cytometry
Nexcelom Bioscience LLC, The Institute of Cancer Reseach

• Celigo Imaging Cytometer is a versatile and powerful tool for 3D tumorspheroid analysis
• Assays such as measuring optimal seeding density for forming tumorspheroids and viability measurement have been demonstrated
• Advanced assays such size measurement, growth inhibition, invasion into matrigel®, migration on to collagen and HUVEC, and tissue invasion that have been performed by manual microscope observation can now be easily quantified using the automated imaging cytometry method
The Power Decoder simulator for the evaluation of pooled shRNA screen performance
GE Healthcare Dharmacon, Inc.

Power Decoder (written in R and Python) simulates shRNA pooled screening experiments in silico to allow for the estimation of a screen’s statistical power. Populations of shRNAs were engineered in such a way that the magnitude of depletion and enrichment was known, then using the negative binomial distribution, an in silico model was developed to successfully resemble data from an actual laboratory experiment.
Application Notes
Aseptic Identification of Polysorbates using Handheld Raman
Rigaku Raman Technologies

As polysorbates are reactive to light and air, packaging is usually in thick, dark, amber bottles. This makes the identification process more difficult, as opening the container could compromise the sterility and quality of these materials. Handheld Raman is capable of identifying these materials without any sampling or preparation.
Cell Culture Media Identification In Biopharmaceuticals With Handheld Raman
Rigaku Raman Technologies

This application note discusses the identification of cell culture media using Raman analysis.
Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
Tecan Group Ltd.

The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.
3D Cell Culture: mimicking real tissue
Tecan Group Ltd.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
3D cell culture: a realistic and reproducible collagen-based environment
Tecan Group

Researchers are increasingly turning to 3D cell culture techniques, due to the improved physiological relevance of the cellular environment. 3D cell cultures offer a more in vivo-like response to stimuli more closely resembling the behavior of animal models or the human body.
Optimized cell culture
Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
Determination of microbial cell viability
Tecan Group Ltd.

The BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells. The assay protocol comprises the addition of the BacTiter-Glo reagent directly to the sample, and subsequent measurement of luminescence; cell washing, removal of culture medium, and multiple pipetting steps are not required.
Stem cell research: Creating blood for high throughput screening
Tecan Group Ltd.

Many different research fields, including investigation of early human development processes, disease research, drug discovery and cellular therapy applications, require appropriate in vitro models which can accurately mimic in vivo processes to provide biologically relevant results. Pluripotent stem cells (PSCs) offer a powerful system for creating in vitro models to address these questions.
Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants
BioTek Instruments

This application note compares the performance of the highest and lowest affinity variants using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader. Sensitive detection of the 22F basal signal allows for use of this variant under a broad range of conditions, providing an assay with both superior dynamic range and sensitivity.
Direct Visualisation, Sizing and Counting of Aggregation in Proteins
NanoSight Ltd

Characterising the state of aggregation in proteins is of paramount importance when trying to understand biopharmaceutical product stability and efficacy. Product quality, both in terms of biological activity and immunogenicity can be highly influenced by the state of protein aggregation.
SELECTBIO

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