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Human iPSC-derived Hepatogytes for 3D Mo...Life of a Scientific Poster...3D Tumor Spheroid-Based Functional Assay...

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Featured ProjectWhitley A95 Workstation
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Featured ProjectMaxCyte® STX™ Scalable Transfection System
MaxCyte® STX™ Scalable Transfection System

Webcasts
Novel Technology Approaches for Capture and Characterization of Circulating Tumor CellsNovel Technology Approaches for Capture and Characterization of Circulating Tumor Cells
Henry Lin,Graduate Student, University of California, speaking at CellTech 2013.
Selection of Label-free Cellular Imaging Applications for their Integration in the Screening StrategySelection of Label-free Cellular Imaging Applications for their Integration in the Screening Strategy
Gerardo Turcatti, Ecole Polytechnique Federale De Lausanne, speaking at Screening Europe 2015.

Market Reports
3D Spheroid Culture Trends 2015
HTStec Limited

This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on three dimensional (3D) spheroid culture carried out in May 2015.
Immunoassay Auto Trends 2014
HTStec

This report comprehensively documents current practices and preferences in immunoassays and to understand future user requirements, particularly with respect to immunoassay automation.
Cell-Based Label-Free Detection Trends 2014
HTStec Limited

This market report summarizes the results of HTStec’s 4th global web-based benchmarking survey on cell-based label-free (LF) detection carried out in July and August 2014. The study also examined in detail the current market landscape, application areas and interest in purchasing new products/systems.
Scientific News
Mitochondria Shown to Trigger Cell Ageing
An international team of scientists has for the first time shown that mitochondria, the batteries of the cells, are essential for ageing.
Cancer Cells Kill Off Healthy Neighbours
Cancer cells create space to grow by killing off surrounding healthy cells, according to UK researchers working with fruit flies.
Editing of Embryos Approved in the UK
The Human Fertilisation and Embryology Authority (HFEA) has approved a research application from the Francis Crick Institute to use new "gene editing" techniques on human embryos.
Microbes Take Their Vitamins
Scientists exploit organisms' needs in order to track 'vitamin mimics' in bacteria.
Machine Learning Uncovers Unknown Bacterial Features
Technique robustly identified characteristic gene expression patterns in response to antibiotics, low oxygen conditions.
CRISPR-Cas9 Gene Editing Advances Again
UC Berkeley researchers have made a major improvement in CRISPR-Cas9 technology that achieves an unprecedented success rate of 60 percent when replacing a short stretch of DNA with another.
Disrupting Cell’s Supply Chain Freezes Cancer Virus
When the cancer-causing Epstein-Barr virus moves into a B-cell of the human immune system, it tricks the cell into rapidly making more copies of itself, each of which will carry the virus.
Why Do Some Infections Persist?
In preparing for the possibility of an antibiotic onslaught, some bacterial cultures adopt an all-for-one/one-for-all strategy that would make a socialist proud, University of Vermont researchers have found.
ASCB: A CELLebration of Cell Biology
The last major congress of the year, ASCB is less a platform for launching new products, but one for confirming and consolidating the trends that have emerged over the past 12 months.
Squeezing Cells into Stem Cells
EPFL scientists have developed a new method that helps cells turn in usable stem cells. The new approach involves “squeezing” cells with a gel, and paves the way for large-scale production of stem cells for medical purposes.
Business News
URMC Partners Indivumed
The University of Rochester Medical Center announced it’s collaborating with Indivumed, a Germany-based company, to establish a bank of human tissues and tumor samples that are expertly preserved and stored for use in cancer research.
InSphero Streamlines 3D Microtissue Business Operations in US
US subsidiary of Swiss-based InSphero AG initiates direct product shipments, progresses towards full-scale US bioproduction capabilities.
InSphero, NCATS to Present Collaborative Research at SLAS2016
Study describes high-throughput compatible screening assay using 3D tumor microtissue models to classify efficacy, toxicity, and mechanism of action for compounds in NIH Oncology Library.
Biovest International Spins off Cell Culture Company
Biovest International Inc. announced that it will spin off its instrument and contract manufacturing offerings into a single wholly-owned subsidiary named “Cell Culture Company, LLC.”
InSphero Adds Head of Sales
Beate Schnell, Ph.D. joins senior team at global leader in 3D cell culture, new hires announced to support US and European growth.
Pall, Kaneka Enter Agreement
Pall Corporation announced the signing of a supply agreement for KANEKA KanCapA Japan for the primary capture of monoclonal antibodies from clarified cell culture.
Irvine Scientific Acquires BioCare Europe
Irvine Scientific, a world leader in the innovation and manufacture of cell culture media, reagents, and medical devices for industrial, clinical and research applications, has announced it has acquired BioCare Europe S.r.l. (BioCare).
Univercells SA Receives €466,500 Grant
Company plans to develop a highly productive biomanufacturing platform based on the results of industrial research that the grant has funded.
Corning, BioCision Announce Agreement
Corning to become global channel distributor for select BioCision products.
CEVEC and BioLamina Combine Technology Leadership
CEVEC adds laminins as CAP®Go application in the field of ex vivo cell culture products for cell therapies.

Events
Downstream Processing World Co
23 Feb 2016
Downstream Processing World Co ...
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Cell Culture World Congress 20
23 Feb 2016
Cell Culture World Congress 20 ...
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The Probiotics Congress: Asia
29 Feb 2016
The Probiotics Congress: Asia ...
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Pittcon 2016
06 Mar 2016
Pittcon 2016 ...
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ePosters
Design considerations for highly specific and efficient synthetic crRNA molecules
GE Healthcare Dharmacon, Inc.

An overview of our rational design algorithm for picking highly functional crRNA sequences in combination with comprehensive specificity analysis.
CellTiter-Glo® 2.0: A Novel Luminescent Cell Viability Assay with Greatly Enhanced Storage Stability
Promega Corporation

Here we report on the attributes of a novel ATP detection reagent for cell viability with all of the assay performance of the previous CellTiter-Glo® Reagent, but now with markedly enhanced stability as a single component in a liquid format. These new features provide for much greater ease-of-use in that storage of the reagent at 4°C eliminates the requirement for reagent thawing and minimizes temperature equilibration time.
Design and Validation of Bioluminescent Assays for 3D Cell Culture Models Poster
Promega Corporation

Cells cultured in 3D model systems often acquire relatively large in vivo-like structures compared to the thickness of a 2D monolayer of cells grown on standard plastic plates.
Testing a Novel Real Time Cell Viability Assay
Promega Corporation

Recently developed assay technologies make it possible to use multi-well plate readers to measure the number of live or dead cells in culture in real time over a period of days. Live cells are measured in real time by adding a reagent containing a shrimp-derived luciferase and a pro-substrate directly to the culture medium. Only viable cells can convert the pro-substrate into a luciferase substrate and generate light.
Picking the best CRISPR-Cas9 targets for functional gene knockout: a machine learning algorithm based on both specificity and functionality
GE Healthcare Dharmacon, Inc.

The CRISPR-Cas9 system has the potential to significantly advance basic and applied research.
Scaffold design, function and over-expression of lentiviral-based microRNAs
GE Healthcare Dharmacon, Inc.

Here we describe the strategy for scaffold design, the importance of an optimal promoter, and demonstrate gene target down-regulation from the over-expression of lentiviral microRNA mimics.
Homology-directed repair with Dharmacon™ Edit-R™ CRISPR-Cas9 and single-stranded DNA oligos
GE Healthcare Dharmacon, Inc.

Here we demonstrate how to perform lipid based transfections for homology directed repair using DharmaFECT Duo, CRISPR-Cas9 reagents and, synthetic DNA donor oligos.
Increasing gene editing efficiencies in eukaryotic cell lines by selection of appropriate CRISPR-Cas9 reagents
GE Healthcare Dharmacon, Inc.

Overview of various CRISPR-Cas9 reagents to provide the highest efficiency of gene editing in your experiments.
Knockdown of p53 by Accell self-delivering siRNA causes inhibition of p53-dependent DNA damage response in IMR-32 neuroblastoma cell line and β-amyloid toxicity in rat cortical neurons
GE Healthcare Dharmacon, Inc.

Here we describe how application of Accell siRNA enabled the development of a high content screening assay in IMR-32 neuroblastoma cells and a whole culture cell viability assay in primary rat cortical neurons.
An Efficient Method for the Incorporation of Molecular Probes at Multiple/Specific sites in RNA: Levulinyl Protection for 2'-ACE ® , 5'-Silyl Oligoribonucleotide Synthesis
GE Healthcare Dharmacon, Inc.

A unique method that uses a levulinate ester as a protecting group to introduce conjugates or molecular probes to virtually any location in a synthetic RNA molecule is discussed. The Levulinyl protecting group is stable in RNA synthesis conditions and can be removed without affecting the other parts of the synthesized RNA. We show the capabilities of this approach with three high-complexity synthesis examples.
Application Notes
Aseptic Identification of Polysorbates using Handheld Raman
Rigaku Raman Technologies

As polysorbates are reactive to light and air, packaging is usually in thick, dark, amber bottles. This makes the identification process more difficult, as opening the container could compromise the sterility and quality of these materials. Handheld Raman is capable of identifying these materials without any sampling or preparation.
Cell Culture Media Identification In Biopharmaceuticals With Handheld Raman
Rigaku Raman Technologies

This application note discusses the identification of cell culture media using Raman analysis.
Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
Tecan Group Ltd.

The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.
3D Cell Culture: mimicking real tissue
Tecan Group Ltd.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
3D cell culture: a realistic and reproducible collagen-based environment
Tecan Group

Researchers are increasingly turning to 3D cell culture techniques, due to the improved physiological relevance of the cellular environment. 3D cell cultures offer a more in vivo-like response to stimuli more closely resembling the behavior of animal models or the human body.
Optimized cell culture
Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
Determination of microbial cell viability
Tecan Group Ltd.

The BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells. The assay protocol comprises the addition of the BacTiter-Glo reagent directly to the sample, and subsequent measurement of luminescence; cell washing, removal of culture medium, and multiple pipetting steps are not required.
Stem cell research: Creating blood for high throughput screening
Tecan Group Ltd.

Many different research fields, including investigation of early human development processes, disease research, drug discovery and cellular therapy applications, require appropriate in vitro models which can accurately mimic in vivo processes to provide biologically relevant results. Pluripotent stem cells (PSCs) offer a powerful system for creating in vitro models to address these questions.
Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants
BioTek Instruments

This application note compares the performance of the highest and lowest affinity variants using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader. Sensitive detection of the 22F basal signal allows for use of this variant under a broad range of conditions, providing an assay with both superior dynamic range and sensitivity.
Direct Visualisation, Sizing and Counting of Aggregation in Proteins
NanoSight Ltd

Characterising the state of aggregation in proteins is of paramount importance when trying to understand biopharmaceutical product stability and efficacy. Product quality, both in terms of biological activity and immunogenicity can be highly influenced by the state of protein aggregation.
SELECTBIO

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