Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Researchers Develop a New Cell and Animal Model of Inflammatory Breast Cancer

Published: Tuesday, April 10, 2012
Last Updated: Tuesday, April 10, 2012
Bookmark and Share
The new model, developed by researchers at the Fox Chase Cancer Center, may provide scientists with a better understanding of the disease and help with developing effective intervention.

Inflammatory breast cancer (IBC) is a very aggressive, often misunderstood type of cancer that is diagnosed more frequently in younger women compared with other types of breast cancer. The five-year survival rate is between 25 and 50 percent—significantly lower than the survival rate for other types of breast cancer. The reason for the poor prognosis is that IBC usually grows rapidly and often spreads quickly to other parts of the body, including the brain, bone and lymph nodes. In an effort to better understand the biology of IBC, researchers at Fox Chase Cancer Center have developed a new cell and animal model that holds promise for providing a detailed understanding of the molecular mechanisms underlying the disease and for developing effective interventions.

“In order for us to improve the treatment of these patients, we need to understand the biology of the disease—why these cells are so aggressive, invade very early on, and are resistant to standard treatments—and this starts with having good laboratory and preclinical models,” says Massimo Cristofanilli, MD, FACP, chairman of Fox Chase’s department of medical oncology and senior investigator for the research, which will be presented at the AACR Annual Meeting 2012 on Wednesday, April 4.

The researchers developed a unique model that recapitulates the aggressive metastasis and cancer stem cell activity associated with poor outcomes in patients with IBC. Understanding of the molecular basis of IBC may help increase the research community’s knowledge of the metastatic process of other types of breast cancers.

“Because there are only a few models of inflammatory breast cancer, it’s important to develop more models of this disease, and ours represents an ideal model to evaluate stem cell-targeting therapies,” says Sandra Fernandez, PhD, assistant research professor at Fox Chase and lead author on the study.

To develop the new disease model, Fernandez, Cristofanilli and their colleagues developed an IRB-approved prospective protocol allowing for the collection of tissue and pleural fluid from patients with advanced IBC. The new cell line, known as FC-IBC02, was established from the pleural fluid collected from a 49-year-old patient whose cells lacked the protein HER2/neu, as well as receptors for the female hormones estrogen and progesterone. About 15 percent of breast cancer patients share these features and, as a result, they do not respond to hormonal therapies and certain medications that target these proteins.

“Currently, the only option to treat these patients is chemotherapy,” Fernandez says. “So it’s important to have a specific model that we can use to test different drugs and see which ones work for this kind of disease.”

Moreover, the researchers grew culture tumor cells derived from the patient’s fluid and found that they contained a large amount of the protein tetraspanin CD151, which controls tumor cell migration and invasion. In addition, these cells formed multicellular spheroids that displayed markers of cancer stem cells, including the marker CD44. When injected into the mammary fat pad of mice, the tumor spheroids rapidly developed into tumors and spread to the lungs.

Furthermore, using the latest CytoScan HD arrays, the FCCC researchers found that these cells have multiple losses and gains across almost the whole genome, a phenomenon known as chromothripsis. In particular, FC-IBC02 cells have an amplification on chromosome 8q where the oncogene MYC is located and a deletion on chromosome 7p where tumor suppressor gene p53 is embedded.  These analysis will identify novel molecular targets to fight the disease. By culturing cells from a large pool of patients, they will look for promising targets that are commonly associated with IBC as well as test new stem cell-targeting drugs that could reduce metastasis.

“I think it’s a major step forward for us as clinicians and scientists to develop better therapies and new diagnostic tools for patients with inflammatory breast cancer,” Cristofanilli says. “We would like to translate our discoveries from bench to bedside very quickly, as these patients really need new treatments.”

Co-investigators on this study include Zhaomei Mu, Lucy Aburto, and Xiaoshen Dong from Fox Chase; Khoi Chu, Kimberly Boley, and Fredika Robertson of the Anderson Cancer Center in Houston, Texas; and Fedor Berditchevski of the University of Birmingham, School of Cancer, Edgbaston, Birmingham, UK.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
DNA Damage Seen in Patients Undergoing CT Scanning
Along with the burgeoning use of advanced medical imaging tests over the past decade have come rising public health concerns about possible links between low-dose radiation and cancer.
Yeast Cells Use Signaling Pathway to Modify Their Genomes
Researchers at the Babraham Institute and Cambridge Systems Biology Centre, University of Cambridge have shown that yeast can modify their genomes to take advantage of an excess of calories in the environment and attain optimal growth.
New Material Forges the Way for 'Stem Cell Factories'
Researchers have discovered the first fully synthetic substrate with potential to grow billions of stem cells. The researchcould forge the way for the creation of 'stem cell factories' - the mass production of human embryonic (pluripotent) stem cells.
New Measurements Reveal Differences Between Stem Cells for Treating Retinal Degeneration
By growing two types of stem cells in a “3-D culture” and measuring their ability to produce retinal cells, a team lead by St. Jude Children’s Research Hospital researchers has found one cell type to be better at producing retinal cells.
Researchers Identify Critical Genes Responsible for Brain Tumor Growth
After generating new brain tumor models scientists have identified the role of a family of genes underlying tumor growth in a wide spectrum of high grade brain tumors.
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
A New Path Towards a Universal Flu Vaccine
New research suggests it may be possible to harness a previously unknown mechanism within the immune system to create more effective and efficient vaccines against this ever-mutating virus.
Potential New Class of Cancer Drugs
Scientists have found a way to stop cancer cell growth by targeting the Warburg Effect, a trait of cancer cell metabolism that scientists have been eager to exploit.
Human Trials of Manufactured Blood Within Two Years
The first human trials of lab-produced blood to help create better-matched blood for patients with complex blood conditions has been announced by NHS Blood and Transplant.
How Anthrax Spores Grow in Cultured Human Tissues
New findings to help predict risk and outcomes of anthrax attacks.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!