Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Well-known Protein Reveals New Tricks

Published: Friday, September 07, 2012
Last Updated: Friday, September 07, 2012
Bookmark and Share
A protein called "clathrin," which is found in every human cell and plays a critical role in transporting materials within them, also plays a key role in cell division.

The discovery, featured on the cover of the Journal of Cell Biology in August, sheds light on the process of cell division and provides a new angle for understanding cancer. Without clathrin, cells divide erratically and unevenly-a phenomenon that is one of the hallmarks of the disease.

"Clathrin is doing more than we thought it was doing," said Frances Brodsky, DPhil, who led the research. Brodsky is a professor in the UCSF Department of Bioengineering and Therapeutic Sciences, a joint department of the Schools of Pharmacy and Medicine, and she holds joint appointments in Microbiology and Immunology, as well as Pharmaceutical Chemistry.

Protein essential for transportation in more than one route

Akin to a three-pronged building block in a child's construction set, clathrin can provide links to create larger complexes. When lots of these proteins are assembled together, they can form tough little cages into which cells packs many of their essential biological molecules-hormones, neurotransmitters, membrane proteins and other payloads that need to be transported throughout the cell.

Once thought to be solely involved in transport inside cells, scientists have uncovered more and more of the protein's hidden functions in the last half-dozen years, including some roles it plays in cell division.

For instance, they learned several years ago about its role in the function of "spindles." Normally when a cell divides, it forms a spindle by laying down tracks of structural proteins, and uses them as scaffolding to separate the cell's DNA (in the form of chromosomes) into two equal collections-one identical set of DNA for each of the new daughter cells. Scientists found that clathrin is involved in stabilizing these spindles.

Now, however, Brodsky and her colleagues have shown that clathrin does even more. They deleted clathrin from cells using a technique called RNA interference, which involves infusing in small genetic fragments that block the cell from making the clathrin. Doing so, Brodsky and her colleagues showed that clathrin stabilizes the structures in dividing cells known as centrosomes.

Tagged with fluorescent chemicals and viewed under a microscope, the centrosomes within a cell that is about to divide look like two glowing eyes peering through the dark. But without clathrin, the team determined, the eyes increase in number.

Brodsky and her colleagues traced this effect to a protein complex formed by one particular component of clathrin called CHC17, which directly stabilizes the centrosome and helps it mature. Deleting CHC17 or chemically inactivating it, led to cells with a strange appearance. These cells contained multiple, fragmented centrosomes instead of the normal two and built abnormal spindles.

This discovery may reveal pathways towards abnormalities of chromosome segregation associated with cancer, said Brodsky.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,800+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Cellular “ORACLs” to Aid Drug Discovery
New approach for finding therapeutics is inspired by face-recognition software.
Wednesday, December 16, 2015
Artificial Kidney Research Gets A Boost
Development of a surgically implantable, artificial kidney — a promising alternative to kidney transplantation or dialysis for people with end-stage kidney disease — has received a $6 million boost.
Monday, November 09, 2015
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
Friday, July 03, 2015
May the Cellular Force be With You
Like tiny construction workers, cells sculpt embryonic tissues and organs in 3D space.
Friday, December 13, 2013
Understanding a Protein’s Role in Familial Alzheimer’s
Researchers have used genetic engineering of human iPSC’s to specifically and precisely parse the roles of a key mutated protein in causing familial Alzheimer's disease (AD).
Monday, November 18, 2013
Developmental Protein Plays Role in Spread of Cancer
A protein used by embryo cells during early development, and recently found in many different types of cancer, apparently serves as a switch regulating metastasis.
Tuesday, June 18, 2013
Nobel Prize Winner Yamanaka Remains at Forefront of Fast-Moving Stem Cell Field
Shinya Yamanaka, MD, PhD, named winner of the 2012 Nobel Prize for Physiology or Medicine, said he was doing some housecleaning when the call came in, and was “very surprised.”
Friday, October 12, 2012
Protein Build-up Leads to Neurons Misfiring
New evidence shows that alpha-synuclein protein build-up inside neurons causes them to not only become "leaky," but also to misfire due to calcium fluxes.
Friday, July 20, 2012
Scientific News
3D Models May Yield Ovarian Cancer Insights
Researchers are developing new tools to decipher ovarian cancer developments through a 3D printing technology.
A Novel Cell Culture Model For Forensic Biology Experiments
Researchers have developed a new cell culture model which provides an efficient research tool in forensic biology.
Mapping Zika’s Routes to Developing Fetus
UC researchers show how Zika virus travels from a pregnant woman to her fetus, and also identified a drug that could stop it.
3D Printing Cartilage
3D bioprinting has successfully manufactured cartilage using bioink sourced from cow cartilage strands.
New Device can Study Electric Field Cancer Therapy
Microfluidic device allows study of electric field cancer therapy through low-intensity fields, preventing malignant cells spreading.
Scientists Culture Elusive Yellowstone Microbe
ORNL scientists have successfully isolated and cultured a Yellowstone sourced acidic hot-spring based microbe.
A 3D Paper-Based Microbial Fuel Cell
Researchers have developed a proof-of-concept 3D paper-based microbial fuel cell (MFC) that could take advantage of capillary action to guide the liquids through the MFC system and to eliminate the need for external power.
Just Gellin’: How To Grow Strong Muscles-On-A-Chip
USC researchers hope to usher in new treatments for patients with muscular dystrophy.
Lasers Carve the Path to Tissue Engineering
A new technique, developed at EPFL, combines microfluidics and lasers to guide cells in 3D space, overcoming major limitations to tissue engineering.
How Cancer Spreads in the Body
Cancer cells appear to depend on an unusual survival mechanism to spread around the body, according to an early study led by Queen Mary University of London.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!