Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

CYTOO’s 2D+ Cell Culture Platform Reproduces in vivo Conditions to Study Tumor Cell Motility

Published: Thursday, October 18, 2012
Last Updated: Thursday, October 18, 2012
Bookmark and Share
New perspectives in oncology, genetics and drug screening.

CYTOO S.A. announced new results that demonstrate the ability of the Company’s 2D+ Cell Culture Platform to reproduce in vivo conditions to analyze tumor cell motility and in particular to study fibrillar ECM-dependent tumor cell-macrophage pairing and migration involved in tumor metastasis. These results have recently been published in the first issue of the journal IntraVital, edited by Landes Bioscience.

The CYTOO 2D+ Cell Culture Platform is based on the use of adhesive micropatterns to guide cell architecture and behavior in culture, in contrast to current 2D cell culture where cells spread and move in an uncontrolled manner. By defining the 2D topology of cell adhesion, 2D+ Technology enables the fine control of the spreading and 3D shape of cultured cells in single- or multi-cellular configurations resulting in control of cell contractility, cell polarity, organelle positioning, or cell division axis.

Researchers Ved Sharma, Brian Beaty, Antonia Patsialou, Dianne Cox, John Condeelis and Robert Eddy from the Albert Einstein College of Medicine, NY, with collaborators Huiping Liu from University of Chicago and Michael Clarke at Stamford School of Medicine, used CYTOOchipsTM Motility to reconstitute an in vitro model of fibrillar tumor extracellular matrix (ECM). The micropatterned 1D adhesive tracks were used to mimic the linear ECM fibers of the tumor microenvironment.

Similar morphologies, behaviors and motility rates were observed in vivo and on micropatterned lines. In particular, tumor cell velocity on 1D substrates was in agreement with the high velocity values of tumor cells on ECM fibers observed in vivo. In contrast, on classical 2D substrates, motility rates were ten fold lower than what can be observed in vivo. On micropatterned lines, the authors could also reproduce the assembly of alternating tumor cells and macrophages identified as streams in vivo, the ability of macrophages to enhance protrusion velocity and average velocity of tumor cells and showed that this effect was dependent on an intact paracrine loop without any additional need of co-factors.

The authors concluded that their “1D micropatterned substrate model more closely approximates the fibrillar nature of the in vivo tumor microenvironment and offers a simple and more appropriate substrate for detailed analyses of cell protrusion, cell-cell pairing and migration than conventional 2D substrates. The data presented here validates the use of micropatterned 1D adhesive substrates to study the fibrillar ECM found within the tumor microenvironment.”

Co-author Robert Eddy commented “It was a surprise that tumor cell and macrophage streaming behavior we observe in the highly complex tumor microenvironment was self-organizing and required no other extracellular cues on 1D adhesive substrates.”


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Olivier Pasquier Joins CYTOO as Chief Commercial Officer
Olivier Pasquier first graduated from the French University Nice-Sophia Antipolis in molecular biology. He then completed his background with a marketing MBA from ESCP Europe.
Monday, March 18, 2013
Scientific News
Women’s Immune System Genes Operate Differently from Men’s
A new technology reveals that immune system genes switch on and off differently in women and men, and the source of that variation is not primarily in the DNA.
DNA Damage Seen in Patients Undergoing CT Scanning
Along with the burgeoning use of advanced medical imaging tests over the past decade have come rising public health concerns about possible links between low-dose radiation and cancer.
Yeast Cells Use Signaling Pathway to Modify Their Genomes
Researchers at the Babraham Institute and Cambridge Systems Biology Centre, University of Cambridge have shown that yeast can modify their genomes to take advantage of an excess of calories in the environment and attain optimal growth.
New Material Forges the Way for 'Stem Cell Factories'
Researchers have discovered the first fully synthetic substrate with potential to grow billions of stem cells. The researchcould forge the way for the creation of 'stem cell factories' - the mass production of human embryonic (pluripotent) stem cells.
New Measurements Reveal Differences Between Stem Cells for Treating Retinal Degeneration
By growing two types of stem cells in a “3-D culture” and measuring their ability to produce retinal cells, a team lead by St. Jude Children’s Research Hospital researchers has found one cell type to be better at producing retinal cells.
Researchers Identify Critical Genes Responsible for Brain Tumor Growth
After generating new brain tumor models scientists have identified the role of a family of genes underlying tumor growth in a wide spectrum of high grade brain tumors.
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
A New Path Towards a Universal Flu Vaccine
New research suggests it may be possible to harness a previously unknown mechanism within the immune system to create more effective and efficient vaccines against this ever-mutating virus.
Potential New Class of Cancer Drugs
Scientists have found a way to stop cancer cell growth by targeting the Warburg Effect, a trait of cancer cell metabolism that scientists have been eager to exploit.
Human Trials of Manufactured Blood Within Two Years
The first human trials of lab-produced blood to help create better-matched blood for patients with complex blood conditions has been announced by NHS Blood and Transplant.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!