Thermo Fisher Scientific Inc. has introduced the Thermo Scientific HyClone HyCell-STEM and HyCell-STEM-FF stem cell culture media, designed to optimize the expansion rate of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) per passage for cell culture applications including cell signaling, drug discovery and therapeutic research.
The new HyCell-STEM media solutions will be showcased this week at the 11th Annual International Society for Stem Cell Research (ISSCR) conference at the Boston Convention and Exhibition Center, Booth #637.
“There are exciting discoveries being made in the stem cell area today, and researchers demand the highest quality products to increase yields and integrity of their cell cultures,” said Roberta Morris, business director for Thermo Fisher Scientific.
Morris continued, “With the introduction of HyCell-STEM and HyCell-STEM-FF, we are offering researchers solutions that increase the rate of stem cell expansion while preserving cell morphology, stemness and pluripotency. Further, scientists may anticipate outstanding recovery after thaw, following cryopreservation of cells.”
HyCell-STEM is designed for use with stem cells on feeder layer cultures, and HyCell-STEM-FF is designed for stem cells in feeder-free conditions.
Both HyCell-STEM and HyCell-STEM-FF offer an increased expansion rate compared to market competitors based on internal testing, yielding as many as twice the cells on every passage.
HyCell-STEM and HyCell-STEM-FF require no adaptation and hESCs and iPSCs can be thawed or seeded directly into the systems. Additionally, robust hESCs and iPSCs grown in HyCell-STEM do not have to be fed daily, saving time, resources and weekend demands for researchers.
For feeder-free culturing with the HyCell-STEM FF, the need of feeder cells is eliminated entirely, and stem cell culture preparation is simplified. Other key features and benefits of the HyCell-STEM and HyCell-STEM-FF systems include:
• Optimized expansion rate of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) per passage
• Healthy stem cell morphology demonstrated by tight clustering and smooth borders of cultured cells
• Preservation of stemness and pluripotency over multiple passages
• Superior post-thaw recovery of stem cells from cryopreservation with cells thawed directly in HyCell-STEM or HyCell-STEM-FF yielding more cells faster