Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
Become a Member | Sign in
Home>News>This Article

Ultra Low Adhesion Plates for Superior 3D Spheroid Cell Culture

Published: Tuesday, March 04, 2014
Last Updated: Tuesday, March 04, 2014
Bookmark and Share
Lipidure®-Coat low adhesion plates and dishes from AMSBIO.

Available from AMSBIO -Lipidure®-Coat low adhesion plates and dishes are shown in cited research to be one of the most effective tools for 3D spheroid cell culture. These new plates and dishes are available in 384-well format optimized for drug screening or other high throughput applications.

Spheroid cell culture is based on the spontaneous formation of an aggregation of cells in an environment where the interactions between cells are superior to the interactions between cells and substrate. This can be achieved by using low-adhesion cell culture conditions.

Lipidure®-Coat surfaces use MPC (2-methacryloyloxtethyl phosphorylcholine), a biocompatible synthetic polymer with the same structure as the phosphorylcholine polar bases that form cell membranes.

As a consequence of the high biocompatibility of this coating, cells will not adhere to the surface of Lipidure®-Coat plates or dishes. Different well-shapes available for 96-well plates (U, V and flat-bottom) can be used to control size, position and density of spheroids.

Studies have shown that Lipidure®-Coat promotes the formation of a single spheroid in each well of U-bottom multi-well plate, while other brands of plate lead to formation of satellite spheroids - indicating a higher degree of cell adhesion is taking place.

Formation of spheroids, tumorspheres, embryonic bodies and neurospheres using LIPIDURE® plates has already been demonstrated for cell types including ES and iPS (human and mouse), NIH3T3, pre-adipocytes, HepG2 and other cancer cell lines as well as primary neuronal cells.

For larger scale assays as well as in applications such as Western blotting or gene expression microarrays - Lipidure®-COAT dishes have produced highly reproducible, top quality results.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Novel Organoid Matrix Enables Long-Term Culture of Human Hepatocytes
Organoid growth matrix to enable long-term culture of genome-stable bipotent stem cells from adult human liver.
Saturday, January 31, 2015
AMSBIO Expands US Operations
Company announces opening of a new North American headquarters in Cambridge, MA.
Wednesday, April 23, 2014
AMSBIO Publishes Comprehensive Cell Culture Handbook
Extensive 48-page handbook is intended for research scientists looking to culture cells in more physiologically relevant environments.
Friday, April 04, 2014
Webinar Addresses 3D Cell-Based Models for Regenerative Medicine
Dr Elad Katz presents new on-demand webinar.
Friday, October 25, 2013
Scientific News
Developing a Gel that Mimics Human Breast for Cancer Research
Scientists at the Universities of Manchester and Nottingham have been funded to develop a gel that will match many of the biological structures of human breast tissue, to advance cancer research and reduce animal testing.
Restoring Vision with Stem Cells
Age-related macular degeneration (AMRD) could be treated by transplanting photoreceptors produced by the directed differentiation of stem cells, thanks to findings published today by Professor Gilbert Bernier of the University of Montreal and its affiliated Maisonneuve-Rosemont Hospital.
Tissue-Engineered Colon from Human Cells
A study by scientists at Children’s Hospital Los Angeles has shown that tissue-engineered colon derived from human cells is able to develop the many specialized nerves required for function, mimicking the neuronal population found in native colon.
Tension Helps Heart Cells Develop Normally in the Lab
Stanford engineers have uncovered the important role tension plays in growing heart cells out of the body.
Urine Excretion From Stem Cell-Derived Kidneys
Researchers report a strategy for enabling urine excretion from kidneys grown from stem cells.
The Black Box at the Beginning of Life
Kyoto University sheds light on the earliest stages of human germ cell development.
Flu Study, on Hold, Yields New Vaccine Technology
Vaccines to protect against an avian influenza pandemic as well as seasonal flu may be mass produced more quickly and efficiently using technology described today by researchers at the University of Wisconsin-Madison.
3D Spheroid Culture Trends
Three dimensional (3D) cell culture has been an area of increasing interest and relevance across a wide breadth of fields for some time.
The Mending Tissue - Cellular Instructions for Tissue Repair
NUS-led collaborative study identifies universal mechanism that explains how tissue shape regulates physiological processes such as wound healing and embryo development.
Most Complete Human Brain Model to Date is a ‘Brain Changer’
Once licensed, model likely to accelerate study of Alzheimer’s, autism, more.

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos