Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Ptychographic Microscope for Three-Dimensional Imaging

Published: Thursday, May 29, 2014
Last Updated: Thursday, May 29, 2014
Bookmark and Share
3D ptychographic method used to visualize 3D specimens up to 34 tomographic sections in depth.

Phase Focus reports on their latest publication released in Optics Express entitled “Ptychographic microscope for three-dimensional imaging.”

Optics Express is the all-electronic journal for optics published by the Optical Society of America and edited by Andrew M. Weiner of Purdue University. It publishes peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.

Label-free 3D live cell imaging is a challenge that remains to be addressed if advances in the development of 3D cell culture constructs for early-stage drug discovery are to reach their full potential. Improved label-free imaging methods are required that can simultaneously analyze all the cells within a 3D specimen rather than simply integrating a cumulative signal response.

In the latest paper based on ptychography, the authors from the universities of Sheffield & York together with UK company, Phasefocus, report on their latest research showing advances towards that goal. It is demonstrated that the technique may be used to visualize 3D specimens up to 34 tomographic sections in depth. These new results compare well with sectioned images collected from a confocal microscope but have the added advantage of strong phase contrast, which removes the need for sample labelling or staining.

The technique is demonstrated on various samples including specimens of common Spirogyra algae and Volvox algae. In both cases, embedded animated images are available to the reader, one of the advantages of an online publication like Optics Express.

The paper also reports images of Arabidopsis Thaliana plant embryo collected using ptychography together with those from a fluorescence confocal microscope; these comparisons clearly illustrate the excellent phase contrast that can be achieved with ptychography. The ptychography images are reconstructions of the optical phase shift throughout the whole specimen, whereas those from the confocal microscope represent the fluorescence intensity from the fluorophores which are embedded within the cell walls only.

In summary, the paper demonstrates how a form of 3D ptychography can be implemented on a conventional microscope platform and can produce optically sectioned images of relatively thick samples on the micron scale. It also demonstrates an increase in the number of sections, or slices, imaged using this method from 5 to 34.

The Phasefocus 3D ptychographic method is unique in that it does not require rotation of the specimen with respect to the illumination source, or any translation of the specimen in the axial direction. This work clearly has potential applications in high contrast and stain-free live cell imaging, as well as in the imaging of thick, strongly scattering specimens using X-rays and electrons.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Mitochondria Shown to Trigger Cell Ageing
An international team of scientists has for the first time shown that mitochondria, the batteries of the cells, are essential for ageing.
Cancer Cells Kill Off Healthy Neighbours
Cancer cells create space to grow by killing off surrounding healthy cells, according to UK researchers working with fruit flies.
Editing of Embryos Approved in the UK
The Human Fertilisation and Embryology Authority (HFEA) has approved a research application from the Francis Crick Institute to use new "gene editing" techniques on human embryos.
Microbes Take Their Vitamins
Scientists exploit organisms' needs in order to track 'vitamin mimics' in bacteria.
Machine Learning Uncovers Unknown Bacterial Features
Technique robustly identified characteristic gene expression patterns in response to antibiotics, low oxygen conditions.
CRISPR-Cas9 Gene Editing Advances Again
UC Berkeley researchers have made a major improvement in CRISPR-Cas9 technology that achieves an unprecedented success rate of 60 percent when replacing a short stretch of DNA with another.
Disrupting Cell’s Supply Chain Freezes Cancer Virus
When the cancer-causing Epstein-Barr virus moves into a B-cell of the human immune system, it tricks the cell into rapidly making more copies of itself, each of which will carry the virus.
Why Do Some Infections Persist?
In preparing for the possibility of an antibiotic onslaught, some bacterial cultures adopt an all-for-one/one-for-all strategy that would make a socialist proud, University of Vermont researchers have found.
ASCB: A CELLebration of Cell Biology
The last major congress of the year, ASCB is less a platform for launching new products, but one for confirming and consolidating the trends that have emerged over the past 12 months.
Squeezing Cells into Stem Cells
EPFL scientists have developed a new method that helps cells turn in usable stem cells. The new approach involves “squeezing” cells with a gel, and paves the way for large-scale production of stem cells for medical purposes.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!