Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
Become a Member | Sign in
Home>News>This Article

Ptychographic Microscope for Three-Dimensional Imaging

Published: Thursday, May 29, 2014
Last Updated: Thursday, May 29, 2014
Bookmark and Share
3D ptychographic method used to visualize 3D specimens up to 34 tomographic sections in depth.

Phase Focus reports on their latest publication released in Optics Express entitled “Ptychographic microscope for three-dimensional imaging.”

Optics Express is the all-electronic journal for optics published by the Optical Society of America and edited by Andrew M. Weiner of Purdue University. It publishes peer-reviewed articles that emphasize scientific and technology innovations in all aspects of optics and photonics.

Label-free 3D live cell imaging is a challenge that remains to be addressed if advances in the development of 3D cell culture constructs for early-stage drug discovery are to reach their full potential. Improved label-free imaging methods are required that can simultaneously analyze all the cells within a 3D specimen rather than simply integrating a cumulative signal response.

In the latest paper based on ptychography, the authors from the universities of Sheffield & York together with UK company, Phasefocus, report on their latest research showing advances towards that goal. It is demonstrated that the technique may be used to visualize 3D specimens up to 34 tomographic sections in depth. These new results compare well with sectioned images collected from a confocal microscope but have the added advantage of strong phase contrast, which removes the need for sample labelling or staining.

The technique is demonstrated on various samples including specimens of common Spirogyra algae and Volvox algae. In both cases, embedded animated images are available to the reader, one of the advantages of an online publication like Optics Express.

The paper also reports images of Arabidopsis Thaliana plant embryo collected using ptychography together with those from a fluorescence confocal microscope; these comparisons clearly illustrate the excellent phase contrast that can be achieved with ptychography. The ptychography images are reconstructions of the optical phase shift throughout the whole specimen, whereas those from the confocal microscope represent the fluorescence intensity from the fluorophores which are embedded within the cell walls only.

In summary, the paper demonstrates how a form of 3D ptychography can be implemented on a conventional microscope platform and can produce optically sectioned images of relatively thick samples on the micron scale. It also demonstrates an increase in the number of sections, or slices, imaged using this method from 5 to 34.

The Phasefocus 3D ptychographic method is unique in that it does not require rotation of the specimen with respect to the illumination source, or any translation of the specimen in the axial direction. This work clearly has potential applications in high contrast and stain-free live cell imaging, as well as in the imaging of thick, strongly scattering specimens using X-rays and electrons.

Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,200+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
Study Implicates Glial Cells in Fragile X Syndrome
The genetic defect behind a common cause of mental retardation affects not only neurons but also the more numerous glial cells in the brain.
Study Finds Key Regulator in Pulmonary Fibrosis
Researchers identify an enzyme that could open the way to therpies for chronic fatal lung disease.
A Step Forward in Building Functional Human Tissues
Researchers at Wyss Institute have bioprinted a tubular 3D renal architecture that recapitulates functions of the kidney.
Stiffening a Blow to Cancer Cells
Researchers develop a way to predict how a tumor tissue's physical properties affect its response to chemotherapy drugs.
Curcumin Shows Promise as Cancer Treatment
When delivered at the correct circadian phase, curcumin demonstrates sustained toxicity in cancer cells and should be considered for use in patient care.
3D-Printing in Science: Conference Co-Staged with LABVOLUTION
LABVOLUTION 2017 will have an added highlight of a simultaneous conference, "3D-Printing in Science".
New Therapeutic Target for Crohn’s Disease
A promising new target for drugs that treat IBD has been identified along with a possible biomarker for IBD severity.
Uncovering Water Bear Resilience
A protein identified in water bears can protect DNA of human cells from lethal doses of radiation damage.
Using Stem Cells to Grow a 3D Lung-in-a-Dish
Researchers have created 3D lung-like tissue from lung-derived stem cells. The tissue can be used to study lung diseases.
Reprogramming Lymph Nodes to Fight MS
Bioengineers work to reprogram lymph node function to fight multiple sclerosis.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,200+ scientific videos