Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
Become a Member | Sign in
Home>News>This Article

Apple Derived Cellulose Scaffolds for 3D Mammalian Cell Culture

Published: Tuesday, July 08, 2014
Last Updated: Tuesday, July 08, 2014
Bookmark and Share
Researchers demonstrate that cellulose scaffolds are a potentially useful biomaterial that can be successfully employed for in vitro 3D cell culture.

There are numerous approaches for producing natural and synthetic 3D scaffolds that support the proliferation of mammalian cells. 3D scaffolds better represent the natural cellular microenvironment and have many potential applications in vitro and in vivo. Here, we demonstrate that 3D cellulose scaffolds produced by decellularizing apple hypanthium tissue can be employed for in vitro 3D culture of NIH3T3 fibroblasts, mouse C2C12 muscle myoblasts and human HeLa epithelial cells. We show that these cells can adhere, invade and proliferate in the cellulose scaffolds. In addition, biochemical functionalization or chemical cross-linking can be employed to control the surface biochemistry and/or mechanical properties of the scaffold. The cells retain high viability even after 12 continuous weeks of culture and can achieve cell densities comparable with other natural and synthetic scaffold materials. Apple derived cellulose scaffolds are easily produced, inexpensive and originate from a renewable source. Taken together, these results demonstrate that naturally derived cellulose scaffolds offer a complementary approach to existing techniques for the in vitro culture of mammalian cells in a 3D environment.

The article, "Apple Derived Cellulose Scaffolds for 3D Mammalian Cell Culture,"  is published online in PLoS ONE and is free to access

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Assessing Anticancer Drugs Efficacy by Amino Acid Metabolomics
Researchers aimed to develop a method that determines the therapeutic efficacy of anticancer drugs based on the changes in amino acid metabolism at the cellular level.
Thursday, July 17, 2014
Advanced Cell Culture Techniques for Cancer Drug Discovery
This review focuses on the culturing of cell lines representative of solid cancers in advanced cell culture conditions.
Thursday, June 12, 2014
Silicon Substrate as a Novel Cell Culture Device for Myoblast Cells
Researchers describe the use of a commercially available monocrystaline silicon PV device to be used as substrate for culturing of C2C12 mammalian cells.
Thursday, June 12, 2014
3D Cell Culture System to Study Tumour-Stroma Interactions
The model allows investigation of tumour-stroma interactions, addressing the importance of having a more in vivo like cell culture model.
Friday, May 16, 2014
A Novel Experimental Hypoxia Chamber for Cell Culture
Researchers have validated an inflatable chamber made of transparent plastic materials as an alternative module for cell culture under hypoxia.
Thursday, February 13, 2014
Recent Advances in 2D and 3D in vitro Systems
The possibilities and limitations of the most useful liver in vitro systems are summarized, including 3D culture techniques, co-cultures with non-parenchymal cells.
Sunday, October 06, 2013
Synthetic Surface for Expansion of Human Mesenchymal Stem Cells in Xeno-Free, Chemically Defined Culture
This study evaluates the ability of the Corning Synthemax Surface to support long-term expansion of hMSCs in serum-free, defined medium.
Friday, August 16, 2013
Isolation and Culture of Smooth Muscle Cells from Human Acute type A Aortic Dissection
The aim of this work was to investigate whether the SMCs of acute TAAD could be isolated and cultured for further research.
Tuesday, April 23, 2013
Effects of 3-D Microwell Culture on Growth Kinetics and Metabolism of Human Embryonic Stem Cells
3-D culture systems have been utilized to enhance growth or differentiation of these hESCs by better mimicking the 3-D nature of in vivo tissue development than standard 2-D systems.
Tuesday, April 23, 2013
Miniaturized Mass-Spectrometry-Based Analysis System for Examination of Conditioned Cell Culture Media
Researchers present an automated system that allows in-line sampling of microliter amounts of extracellular conditioned cell culture media, preparing them for MALDI MS analysis.
Monday, November 05, 2012
Scientific News
Novel Tumor Treatment
In the first published results from a $386,000 National Cancer Institute grant awarded earlier this year, a paper by Scott Verbridge and Rafael Davalos has been published.
Personalized Drug Screening for Multiple Myeloma Patients
A personalized method for testing the effectiveness of drugs that treat multiple myeloma may predict quickly and more accurately the best treatments for individual patients with the bone marrow cancer.
Cancer-Fighting Tomato Component Traced
The metabolic pathway associated with lycopene, the bioactive red pigment found in tomatoes, has been traced by researchers at the University of Illinois.
Some Gut Microbes May Be Keystones of Health
University of Oregon scientists have found that strength in numbers doesn’t hold true for microbes in the intestines. A minority population of the right type might hold the key to regulating good health.
The Life Story of Stem Cells
A model analyses the development of stem cell numbers in the human body.
Novel Stem Cell Line Avoids Risk of Introducing Transplanted Tumors
Progenitor cells might eventually be used to repair or rebuild damaged or destroyed organs.
Tissue Engineers Recruit Cells to Make Their Own Strong Matrix
Extracellular matrix is the material that gives tissues their strength and stretch. It’s been hard to make well in the lab, but a Brown University team reports new success. The key was creating a culture environment that guided cells to make ECM themselves.
Towards Patient-Specific Drug Screening
A new breakthrough by the 3D stem cell printing team at Heriot-Watt could pave the way to individually tailored drug testing regimes, both reducing the need for animal testing and ensuring that patients receive drugs which are most effective for their individual needs.
Artificial Kidney Research Gets A Boost
Development of a surgically implantable, artificial kidney — a promising alternative to kidney transplantation or dialysis for people with end-stage kidney disease — has received a $6 million boost.
Improving the Efficiency of Red Blood Cell Production
Study points to way of significantly reducing cost of laboratory-produced cells.

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos