Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
Become a Member | Sign in
Home>News>This Article

CCRM Uses ambr15 for Pluripotent Stem Cell Expansion

Published: Tuesday, August 05, 2014
Last Updated: Tuesday, August 05, 2014
Bookmark and Share
New protocols increase stem cell expansion outputs and improve differentiation into cardiomyocytes.

TAP Biosystems has announced that the ambr15™ micro bioreactor system is being used at the Centre for Commercialization of Regenerative Medicine (CCRM), Canada to rapidly develop methods for culturing stem cells in bioreactors, thus shortening timelines for successfully scaling-up and manufacturing more cost-effective cell therapies and regenerative medicines.

Scientists at CCRM have utilized the ambr15 micro bioreactor system to optimize methods for forming three-dimensional aggregates of pluripotent stem cells (PSCs) for cell expansion, and differentiating the aggregates into enriched cardiomyocytes populations.

In the new protocol for PSC aggregate culture, PSCs were incubated in media supplemented with FGF-2 and ROCKi, in six well plates on orbital shakers for 24 hours. The cell suspensions were transferred to ambr15 micro bioreactors or left on the orbital shakers where they were incubated for a further 48 hours.

Different agitation speeds of 300rpm or 600rpm were set for the ambr15 micro bioreactors and 70rpm for the controls. The results showed that cells cultured in the ambr15 micro bioreactors produced aggregates of a more consistent size distribution with greater total cell expansion compared to controls. Preliminary data indicate using ambr15 micro bioreactors to optimize parameters, such as agitation speed, could improve cell yield for PSC culture.

For differentiation, PSCs in ambr15 micro bioreactors were induced to cardiac cells over 14 days. The results showed that culture in the ambr15 significantly improved cardiomyocyte yield compared to the orbital shaker controls.

“These preliminary data provide promising leads on key parameters for further optimization of pluripotent stem cell culture and production of cardiomyocytes," explains Dr. Nick Timmins, Director Product and Process Development, Centre for Commercialization of Regenerative Medicine. "The ambr has been instrumental in enabling rapid translation of these cultures to controlled bioreactors, and lays the foundations for successful scale-up to clinically meaningful cell quantities in the future."

The full methods and results of the CCRM studies are available in a poster that can be requested via the link:

Dr Barney Zoro, ambr Product Manager at TAP Biosystems stated: "A challenge for the regenerative medicine industry is to develop cell culture processes that are well characterized and can be scaled up for production to ensure clinical and commercial success. Producing PSC aggregates is one example of how to culture non-adherent stem cells in bioreactors to enable generation of clinical scale cell yields.”

Zoro continued: “The research by the CCRM scientists with the ambr15 is very exciting because it shows that this technology not only allows modelling of stem cell expansion and differentiation but also optimization of stem cell related parameters. Utilizing ambr15 for these applications reduces reliance on costly and time consuming bench top bioreactors, allowing cell therapy scientists to reduce costs and timelines for their process development work. This will help them develop robust, cost-effective manufacturing processes and means affordable regenerative therapies for large patient numbers could become a reality.”

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Merck and TAP Biosystems Finalists for “Best Collaboration Award”
Recognizing ambr250 as a breakthrough technology for process development.
Wednesday, October 08, 2014
TAP and Gallus Co-host Free Webinar
Explaining the benefits of using ambr15 microbioreactors for DoE.
Wednesday, October 01, 2014
New Webinars Explore Feed Strategy Challenges Using Mini Bioreactors
Detailing how to achieve consistent feeding regimes for reproducible scale-up.
Thursday, September 25, 2014
New Webinar on Mimicking Perfusion Culture Using Micro Bioreactors
Presents validation data for improving media screening and process optimization.
Friday, February 14, 2014
TAP Biosystems Presents New Data on 3D Cell Culture Research
Discussing the application of RAFT 3D models in oncology, toxicology and neuroscience cell-based screening programmes.
Monday, January 20, 2014
Collaboration between TAP Biosystems and UCL to Develop Biomimetic 3D Cancer Models
TAP Biosystems announced new collaboration to develop solid tumour tissue models using its RAFT 3D cell culture system for use in drug discovery applications.
Friday, October 12, 2012
Scientific News
Novel Tumor Treatment
In the first published results from a $386,000 National Cancer Institute grant awarded earlier this year, a paper by Scott Verbridge and Rafael Davalos has been published.
Personalized Drug Screening for Multiple Myeloma Patients
A personalized method for testing the effectiveness of drugs that treat multiple myeloma may predict quickly and more accurately the best treatments for individual patients with the bone marrow cancer.
Cancer-Fighting Tomato Component Traced
The metabolic pathway associated with lycopene, the bioactive red pigment found in tomatoes, has been traced by researchers at the University of Illinois.
Some Gut Microbes May Be Keystones of Health
University of Oregon scientists have found that strength in numbers doesn’t hold true for microbes in the intestines. A minority population of the right type might hold the key to regulating good health.
The Life Story of Stem Cells
A model analyses the development of stem cell numbers in the human body.
Novel Stem Cell Line Avoids Risk of Introducing Transplanted Tumors
Progenitor cells might eventually be used to repair or rebuild damaged or destroyed organs.
Tissue Engineers Recruit Cells to Make Their Own Strong Matrix
Extracellular matrix is the material that gives tissues their strength and stretch. It’s been hard to make well in the lab, but a Brown University team reports new success. The key was creating a culture environment that guided cells to make ECM themselves.
Towards Patient-Specific Drug Screening
A new breakthrough by the 3D stem cell printing team at Heriot-Watt could pave the way to individually tailored drug testing regimes, both reducing the need for animal testing and ensuring that patients receive drugs which are most effective for their individual needs.
Artificial Kidney Research Gets A Boost
Development of a surgically implantable, artificial kidney — a promising alternative to kidney transplantation or dialysis for people with end-stage kidney disease — has received a $6 million boost.
Improving the Efficiency of Red Blood Cell Production
Study points to way of significantly reducing cost of laboratory-produced cells.

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos