|A New Approach to Increase Yields and Improve Functionality of Recombinant Proteins|
Antti Vasala, BioSilta Oy, Oulu, Finland
The ability to express and extract fully functional proteins in sufficient quantities from bacterial cultures is a prerequisite for many projects in which recombinant proteins are required for structural studies, functional characterization, as assay components or for other applications. However, low yield, poor solubility and lack of functionality are often associated with, what should be, a routine procedure.
|A Novel Synthetic Peptide as a Tool for Microvesicles / Exosomes isolation ©|
We have engineered and validated a synthetic peptide (Vn96*) with specific affinity for canonical heat shock proteins (HSPs) as a tool for rapid isolation of Exosomes or extracellular microvesicles (together referred to as eMVs here) from cell culture media, human and animal body-fluids.
|Chia-Hsun Hsieh and Sanger Hung-Chih Lin win at CellTech 2012|
Sanger Hung-Chih Lin, Chia-Hsun Hsieh, Hung-Chih Hsu, Hung-Ming Wang, Chi-Ya Huang, Min-Hsien Wu and Ching-Ping Tseng
Chia-Hsun Hsieh and Sanger Hung-Chih Lin win at CellTech 2012
|Determination and Comparison of Specifics of Nucleus Pulposus Cells of Human Intervertebral Disc in Alginate and Chitosan– Gelatin Scaffolds|
Masoud Ghorbani, Hamid Bahramian Renani , Batool Hashemibeni Beni, Zeinab Karimi
In this work we studied specefices of NP cells obtained from nucleus pulposus by collagenase enzymatic hydrolysis obtained from patients undergoing open surgery.
|Transporter-based In Vitro-In Vivo Extrapolation (IVIVE)|
Viera Lukacova, Michael B. Bolger, Walter S. Woltosz, Neil J. Parrott, Agnès Poirier and T. Lavè
Mechanistic analysis of in vitro measurements to obtain values of kinetic parameters for drug interactions with transporters, as well as for passive diffusion through cell membranes.
|The Mesenchymal Stem Cells Bioengineered Tissue Using Cobalt-60 Radiation|
Mosca, RC., Isaac, C., Mathor, MB
The biological effects of gamma radiation by cobalt-60 sources on epidermal-dermal matrix have not been fully elucidated when mesenchymal stem cells cultured add in. This poster shows how scientists standardized the keratinocytes and adipose-derived stem cell (ADSC) culture procedure before constructing a bioengineered dermal-epidermal equivalent populated by keratinocytes and ADSC cultured cells.
|Effect of Culture Media and Serum Supplementation on the Development of in vitro Fertilized Buffalo Embryos|
D Kumar, T Anand, P Palta and M.S. Chauhan
This poster compares the development of buffalo embryos in simple and complex culture media and aims to determine the effects of serum supplementation on the development of buffalo embryos.
|An Emerging Revolution in Automated 3D Cell Culture|
Cal Trepagnier, Stacey Szymanski, Kevin Huff, Alision Rush, Richard Peltier, Berta strulovici, Rebecca Lobo and Robin Felder
Market pressure exists for new paradigms to meet the demands for more rapid and reproducible cell production to keep up with advances in cell-based assays used in drug discovery and development. Novel automation platforms enable high productivity on three dimensional surfaces.
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