|Idebenone Inhibits Cell Proliferation by Blocking of ANO1/ TMEM16A Chloride Channel in Adenocarcinoma Cells|
Idebenone significantly reduced cell proliferation and induced apoptosis in PC-3, CFPAC-1, HT-29, T-84 and Calu-3 cells having CaCCs activities. These data suggest that idebenone, an ANO1/CaCC inhibitor, has potential for use in cancer therapy.
|Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry|
SC Cribbes, S Brightwell, K Kaji
Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.
|Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method|
Sarah Kessel, Eric Sincoff, Olivier Dery
This study demonstrates that the cell culture assay for tumorsphere size was successfully adapted to 384-well plate for a 3D spheroid assay using U-bottom low attachment 384-well and the Celigo Automated Imager for image acquisition and analysis. Moreover, the utilization of an imager provided insight on sphere and cell fate after long term drug treatment.
|Using An in‐vitro Human Airways Model to Study the Effects of e‐cigarettes on Bronchial Epithelial Cells|
Vasanthi Bathrinarayanan, P.1 Marshall, L.J.2 Leslie, L.J.1
The prime aim of our study is to use in-vitro human airway models as an effective alternative to replace animals in traditional/E-cigarette research.
|"In-vitro studies on hydroxamic acid- CT DNA binding" |
In vitro studies of five the interaction between five different derivatives of hydroxamic acids, N-phenyl 2,4dichloro phenoxybutyro, N-m-tolyl 2,4 dichlorophenoxyglutero, N-m-tolyl-4-chlorophenoxyaceto, N-m-chloro-phenyl-tertiarybutylbenzo and N-p-tolyl-iso-valero hydroxamic acids and calf thymus DNA was investigated under simulated physiological condition.
|High Titre BacMam viruses improve transduction efficiency of mammalian cells|
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King
We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.
|Identification and Characterisation of Novel Positive Allosteric Modulators of the Galanin 2 Receptor|
An HTS against GalR2 was carried out to find novel PAMs to treat neuropathic pain.
|The Longest Way Round is the Shortest Way Home: HTS Assay Development for Complex Multi-domain Protein Kinases|
Doris Hafenbrandl, Vanessa Nardese, Maria Cristina Sidoli, Valeria Wanke, Mariantonietta Rubino and Daniele Carettoni
This study strongly supports the possibility to overcome the major bottlenecks in the production of long kinase recombinant forms, in order to perform drug discovery programmes with proteins more closely preserving the structural and functional properties of the native enzymes.
|Automated Fluorescence Detection and Imaging of RNA Species in Live Cells|
Paul Held, Victor Koong, Don Weldon, Peter Banks
This poster describes the detection and quantification of RNA species in Live cells through automated imaging.
|Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses|
Brad Larson 1, Grant Cameron 2, Nicolas Pierre 3, and Peter Banks 1
Here we demonstrate an in vitro HTRF® microplate assay that can quantify total, as well as phosphorylated elF4E.
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