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Development of a novel xeno-free medium for feeder-free culture of human stem cells
Annand R; Okuda Y; Inamura M

A new xeno-free medium (ReproXFTM) has been developed for feeder-free culture of stem cells.

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Novel culture medium using a small-molecule agonist of thrombopoetin receptor
Hondo M1; Nishino T2; Inamura M1

ReproHSC medium for the culture of hematopoietic stem cells. facilitates the culture and expansion of CD34+/CD38- cells that retain their HSC properties

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A novel multi-organ microfluidic chip: on the way to the complexity of a living organism
Timur R Samatov, Svetlana A Tonevitskaya, Natalya Pulkova, Evgeny A Tonevitsky

A novel physiologically relevant multi-organ chip is developed capable of culturing up to six different organotypic models integrated into a single microfluidic circuit.

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Continuous Collection of Stem Cells from a Human Placenta Perfusion Co-Culture
John J.S. Cadwell & James C. Hardy

The potential for human placenta-derived cells to produce stem cells in a hollow fiber bioreactor co-culture system was investigated.

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Identification of microRNA targets using microRNA modulation techniques and gene expression arrays
Emily M. Anderson, Maren Mayer, Kevin Sullivan, Barbara Robertson, Žaklina Strezoska, Annaleen Vermeulen, and Devin Leake

By examining the overlap of messages down-regulated by miRNA mimics and up-regulated by miRNA inhibitors, we robustly identify miRNA-regulated messages, many of which have canonical seed matches and some which are not identied by standard target prediction programs.

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Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry
Leo L. Chan, Srinivas S. Somanchi, Kelsey J. Rosbach, and Dean A. Lee.

In this work, we demonstrate a novel high throughput cytotoxicity detection assay using the Celigo Imaging Cytometry method. Utilizing imaging cytometry, direct cell counting of live fluorescent target cells can be performed, which is a direct method for assessment of cytotoxicity.

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Idebenone Inhibits Cell Proliferation by Blocking of ANO1/ TMEM16A Chloride Channel in Adenocarcinoma Cells
Yohan Seo

Idebenone significantly reduced cell proliferation and induced apoptosis in PC-3, CFPAC-1, HT-29, T-84 and Calu-3 cells having CaCCs activities. These data suggest that idebenone, an ANO1/CaCC inhibitor, has potential for use in cancer therapy.

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Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry
SC Cribbes, S Brightwell, K Kaji

Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.

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Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method
Sarah Kessel, Eric Sincoff, Olivier Dery

This study demonstrates that the cell culture assay for tumorsphere size was successfully adapted to 384-well plate for a 3D spheroid assay using U-bottom low attachment 384-well and the Celigo Automated Imager for image acquisition and analysis. Moreover, the utilization of an imager provided insight on sphere and cell fate after long term drug treatment.

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Using An in‐vitro Human Airways Model to Study the Effects of e‐cigarettes on Bronchial Epithelial Cells
Vasanthi Bathrinarayanan, P.1 Marshall, L.J.2 Leslie, L.J.1

The prime aim of our study is to use in-vitro human airway models as an effective alternative to replace animals in traditional/E-cigarette research.

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Scientific News
A New Wrinkle For Cell Culture
Researchers at Brown University have developed an advanced technique for cell culturing that uses sheets of wrinkled graphene to mimic the complex 3-D environment inside the body.
A New Lease on Life for Prostate Tissue
Rutgers Cancer Institute of New Jersey research results in development of organoid prostate models
A New Tool for Understanding ALS: Patients’ Brain Cells
Researchers create a free public library of versatile stem cells from ALS patients.
MIPT Researchers Grow Cardiac Tissue on “Spider Silk” Substrate
Researchers discuss their findings in an article that has recently come out in the journal PLOS ONE.
Scientists Make No Bones About First Study of Osteocyte Cultures on Space Station
Investigation team will analyze the effects of microgravity on this type of bone cell.
Amniotic Stem Cells Demonstrate Healing Potential
Rice University, Texas Children’s Hospital study proves cells promote vasculature in hydrogel therapy.
How the Human Immune System Keeps TB at Bay
Scientists create model of host, bacteria behavior in latent TB infection.
A Single-Cell Breakthrough
UNC School of Medicine scientist Scott Magness and collaborators use their newly developed technology to dissect properties of single stem cells. The advancement will allow researchers to study gastrointestinal disorders and cancers like never before.
‘Mini-Lungs’ Grown To Aid The Study Of Cystic Fibrosis
'Mini-lungs’ have been created using stem cells derived from skin cells of patients with cystic fibrosis.
Designing A Better Way To Study Stomach Flu
Texas Medical Center team aims to improve research of gastrointestinal disease.
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