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Monday, January 26, 2015
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Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry
SC Cribbes, S Brightwell, K Kaji

Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.

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Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method
Sarah Kessel, Eric Sincoff, Olivier Dery

This study demonstrates that the cell culture assay for tumorsphere size was successfully adapted to 384-well plate for a 3D spheroid assay using U-bottom low attachment 384-well and the Celigo Automated Imager for image acquisition and analysis. Moreover, the utilization of an imager provided insight on sphere and cell fate after long term drug treatment.

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Using An in‐vitro Human Airways Model to Study the Effects of e‐cigarettes on Bronchial Epithelial Cells
Vasanthi Bathrinarayanan, P.1 Marshall, L.J.2 Leslie, L.J.1

The prime aim of our study is to use in-vitro human airway models as an effective alternative to replace animals in traditional/E-cigarette research.

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"In-vitro studies on hydroxamic acid- CT DNA binding"
Rubi Khilari

In vitro studies of five the interaction between five different derivatives of hydroxamic acids, N-phenyl 2,4dichloro phenoxybutyro, N-m-tolyl 2,4 dichlorophenoxyglutero, N-m-tolyl-4-chlorophenoxyaceto, N-m-chloro-phenyl-tertiarybutylbenzo and N-p-tolyl-iso-valero hydroxamic acids and calf thymus DNA was investigated under simulated physiological condition.

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High Titre BacMam viruses improve transduction efficiency of mammalian cells
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King

We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.

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Identification and Characterisation of Novel Positive Allosteric Modulators of the Galanin 2 Receptor
Hayley Jones

An HTS against GalR2 was carried out to find novel PAMs to treat neuropathic pain.

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The Longest Way Round is the Shortest Way Home: HTS Assay Development for Complex Multi-domain Protein Kinases
Doris Hafenbrandl, Vanessa Nardese, Maria Cristina Sidoli, Valeria Wanke, Mariantonietta Rubino and Daniele Carettoni

This study strongly supports the possibility to overcome the major bottlenecks in the production of long kinase recombinant forms, in order to perform drug discovery programmes with proteins more closely preserving the structural and functional properties of the native enzymes.

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Automated Fluorescence Detection and Imaging of RNA Species in Live Cells
Paul Held, Victor Koong, Don Weldon, Peter Banks

This poster describes the detection and quantification of RNA species in Live cells through automated imaging.

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Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses
Brad Larson 1, Grant Cameron 2, Nicolas Pierre 3, and Peter Banks 1

Here we demonstrate an in vitro HTRF® microplate assay that can quantify total, as well as phosphorylated elF4E.

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Validation of a 3-Dimensional Human Liver Microtissue Model for Long-term Hepatotoxicity Studies
Brad Larson1, Stewart Hunt2, Timothy Moeller3, Diana Long4, and Peter Banks1

Non-steroidal anti-inflammatory drugs (NSAIDs) are a class of drug commonly used as analgesics and antipyretics, as well as for management of rheumatological disorders. They are one of the most highly prescribed drug families around the world, and consequently, along with antimicrobial agents, are the most frequent causes of druginduced liver injury (DILI) (Bjornsson et al., 2010).

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Showing Results 1 - 10 of 49
Scientific News
New Revolutionary Culturing Technique for Liver and Pancreas
Culturing of mini-guts from single mouse intestine stem cells.
First Contracting Human Muscle Grown in Laboratory
Researchers at Duke University report the first lab-grown, contracting human muscle, which could revolutionize drug discovery and personalized medicine.
How Bacteria Control Their Size
By monitoring thousands of individual bacteria scientists discovered how they maintain their size from generation to generation.
New Technology Makes Tissues, Someday Maybe Organs
A new device for building large tissues from living components of three-dimensional microtissues borrows on ideas from electronics manufacturing.
3-D Culture System for Pancreatic Cancer has Potential to Change Therapeutic Approaches
Organoid technology with human tissue provides a model for full progression of the disease.
Adapting Influenza for Vaccine Mass Production
The ability to grow influenza in cell culture rather than chicken eggs paves the way for mass production of the vaccine.
Tailor-Made Cancer Treatments? New Cell Culture Technique Paves The Way
Technique grew cells from 73% of patients in the study, more than three times as effective as previous methods.
Recreating Stem Cells From Deceased Patients to Study Present-Day Illnesses
Cedars-Sinai team remakes brain and gut stem cells in a dish to test potential therapies for debilitating and fatal diseases.
Protein Key To The Development Of Blood Stem Cells Identified
Understanding the self-replication mechanisms is critical for improving stem cell therapies for blood-related diseases and cancers.
Pain In A Dish
Turning skin cells into pain-sensing neurons.

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