|Quantitative Cell-Based Bioassays for Individual and Combination Immune Checkpoint Immunotherapy Targets|
Zhi-jie Jey Cheng, Jamison Grailer, Pete Stecha, Jun Wang, Jim Hartnett, Frank Fan, and Mei Cong
Immune checkpoint receptors are promising new immunotherapy
targets for the treatment of a variety of diseases including cancer and
autoimmune-mediated disorders. We developed a suite of cell-based
bioluminescent reporter bioassays for individual and combination
immune checkpoint immunotherapy targets including: PD-1 (PD-L1 or PD-L2), CTLA-4, LAG-3, TIGIT, PD-1+TIGIT, GITR, 4-1BB, CD40, and OX40.
|A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources|
Mira Genser-Nir, Sharon Daniliuc, Marina Tevrovsky, Roni Hazan Brill, Yuliya-Yael Miropolski, David Fiorentini.
An overview of a novel SF, XF differentiation system which enables achieving defined conditions for rapid generation of differentiated hMSCs towards tissue engineering and drug screening applications
|A Synthetic CRISPR-Cas9 System for Homology-directed Repair|
John A. Schiel, Maren M. Gross, Emily M. Anderson*, Eldon T. Chou, Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA
Synthetic, dual-RNA-encoded Cas9 is used for precise homology-directed repair (HDR) gene engineering. Both short and long (GFP) inserts are covered.
|Phenotypic Cell-Based Screening of a High Content Imaging Cell Health Assay using the IN Cell Analyzer 2000|
Zaynab Neetoo-Isseljee, Chido Mpamhanga, David Tickle, Debra Taylor and Janet Brownlees
A High Content Imaging Cell Health assay has been established in-house for phenotypic High Content Screening using the IN Cell Analyzer 2000 and Genedata Screener analysis software. We describe the assay development and initial screening of the FDA set in HepG2 cells for validation of the assay.
|Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay|
Stefanie Buesch1 , John Langer2 , Sabine Schaepermeier1, Lubna Hussain2, Jeffrey Bergeron3, Volker Vogel1, Jenny Schroeder1
This poster explains how to measure cell viability easily in 3D cell cultures using the ViaLight™ Plus BioAssay.
|Cell Culture and Cell Analysis using the Real Architecture for 3D Tissue (RAFT™) Culture System|
Cecile Villemant1 , Sabine Schäpermeier2 , Stefanie Büsch2 , John Langer3 , Theresa D’Souza3 , Lubna Hussain3 , Grant Cameron1 , Volker Vogel2 , Jenny Schroeder2
This poster explains how standard analysis techniques, like fluorescence microscopy, can be applied easily to RAFT™ 3D Cell Cultures.
|Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System|
John Langer1 , Jenny Schroeder2 , Lubna Hussain1 , Claudia Schwartz2 and Theresa D’Souza1
The RAFT™ 3D cell culture system provides a valuable tool to investigate different cell types singularly or in co-cultures in an in-vivo like collagen based microenvironment.
|Pharmacological Responses in Cultured Human iPSC-Derived Cortical Neurons Using Multi-Electrode Array |
Aoi Odawara (1,2), Hiroki Katoh (1), Naoki Matsuda (1), Karolina Szczesna (3), Yichen Shi (3), Ryan Arant (4), Hideyasu Jiko (4), Ikuro Suzuki (1)
The functional network of human induced pluripotent stem cell (hiPSC)-derived neurons is a potentially powerful in vitro model for evaluating disease mechanisms and drug responses. However, the culture time required for the full functional maturation of individual neurons and networks is uncertain. We investigated the development of spontaneous electrophysiological activity and pharmacological responses for over 1 year in culture using multi-electrode arrays (MEAs).
|Translational Research of oral Neural Crest-Derived Stem Cells (oNCSCs) in Regenerative Dentistry|
Grimm W.-D1,2,4, S. N. Alekseenko9, D. V. Bobryshev1, W. Duncan11, B. Giesenhagen3, E. Gubareva9, Sema S. Hakki6, O.V. Pershina7, I. Schau4, S. V. Sirak1, E.G. Skurikhin7, A. A. Sletov1, F. Witte10, G. Varga8, O. V. Vladimirova1, M.A. Vukovic2, D. Widera5
In this review poster, we summarize current knowledge on the oral neural crest-derived stem cell populations (oNCSCs) and discuss their potential in regenerative periodontology as a part of regenerative dentistry.