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Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry
Leo L. Chan, Srinivas S. Somanchi, Kelsey J. Rosbach, and Dean A. Lee.

In this work, we demonstrate a novel high throughput cytotoxicity detection assay using the Celigo Imaging Cytometry method. Utilizing imaging cytometry, direct cell counting of live fluorescent target cells can be performed, which is a direct method for assessment of cytotoxicity.

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Idebenone Inhibits Cell Proliferation by Blocking of ANO1/ TMEM16A Chloride Channel in Adenocarcinoma Cells
Yohan Seo

Idebenone significantly reduced cell proliferation and induced apoptosis in PC-3, CFPAC-1, HT-29, T-84 and Calu-3 cells having CaCCs activities. These data suggest that idebenone, an ANO1/CaCC inhibitor, has potential for use in cancer therapy.

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Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry
SC Cribbes, S Brightwell, K Kaji

Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.

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Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method
Sarah Kessel, Eric Sincoff, Olivier Dery

This study demonstrates that the cell culture assay for tumorsphere size was successfully adapted to 384-well plate for a 3D spheroid assay using U-bottom low attachment 384-well and the Celigo Automated Imager for image acquisition and analysis. Moreover, the utilization of an imager provided insight on sphere and cell fate after long term drug treatment.

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Using An in‐vitro Human Airways Model to Study the Effects of e‐cigarettes on Bronchial Epithelial Cells
Vasanthi Bathrinarayanan, P.1 Marshall, L.J.2 Leslie, L.J.1

The prime aim of our study is to use in-vitro human airway models as an effective alternative to replace animals in traditional/E-cigarette research.

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"In-vitro studies on hydroxamic acid- CT DNA binding"
Rubi Khilari

In vitro studies of five the interaction between five different derivatives of hydroxamic acids, N-phenyl 2,4dichloro phenoxybutyro, N-m-tolyl 2,4 dichlorophenoxyglutero, N-m-tolyl-4-chlorophenoxyaceto, N-m-chloro-phenyl-tertiarybutylbenzo and N-p-tolyl-iso-valero hydroxamic acids and calf thymus DNA was investigated under simulated physiological condition.

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High Titre BacMam viruses improve transduction efficiency of mammalian cells
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King

We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.

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Automated Fluorescence Detection and Imaging of RNA Species in Live Cells
Paul Held, Victor Koong, Don Weldon, Peter Banks

This poster describes the detection and quantification of RNA species in Live cells through automated imaging.

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Identification and Characterisation of Novel Positive Allosteric Modulators of the Galanin 2 Receptor
Hayley Jones

An HTS against GalR2 was carried out to find novel PAMs to treat neuropathic pain.

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Showing Results 11 - 20 of 60
Scientific News
A Patient’s Budding Cortex — In A Dish?
Networking neurons thrive in 3-D human “organoid”
Researchers use Nanoparticle Tracking Analysis to Study Extracellular Vesicles
Scientists are working to find a way deliver a specific microRNA species capable of retarding the growth of tumor cells.
Tiny Spheres Of Human Cells Mimic The Brain
Researchers have figured out how to create spheres of neuronal cells resembling the cerebral cortex, making functional human brain tissue available for the first time to study neuropsychiatric diseases such as autism and schizophrenia.
Study Maps Prenatal Cells
Babies’ sex cells unprotected during pregnancy. Research delivers new data for stem cell scientists to more accurately study infertility.
Bold Steps Toward Engineering New Lungs
ASU researchers are working to improve aspects of lung engineering that may in the future contribute to providing a nearly limitless supply of donor organs, ideally matched to their recipients, or to repairing damaged lungs.
Bacteria Cooperate to Repair Damaged Siblings
New research unearths the unique ability of a certain type of soil borne bacteria to repair nearby damaged cells.
UB and Roswell Park receive $1.85M Grant to Launch Stem Cell Research Program
The program will bring together 18 faculty members to advance translation of stem cell breakthroughs into cell therapies.
Solving Streptide from Structure to Biosynthesis
Researchers reveal new information about how bacteria communicate via the protein, streptide.
Bioprinting in 3D: Looks Like Candy, Could Regenerate Nerve Cells
Tolou Shokuhfar and colleagues are developing techniques using 3D bioprinting to generate human tissue.
Possible Treatment for Lethal Pediatric Brain Cancer
NIH-funded preclinical study suggests epigenetic drugs may be used to treat leading cause of pediatric brain cancer death.
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