Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay

Conventional in vitro assays are based on cells grown on two-dimensional (2D) substrates, which are not representative of a true in vivo cell environment. Three-dimensional (3D) cell culture methods, in contrast, allow cells to grow in structures resembling more the in vivo environment. Cells can develop cell-cell and cell-extracellular matrix (ECM) interactions in 3D.

While 3D cultures more accurately approach the in vivo environment, it might be difficult to analyze cells in 3D. However, this is not always the case. This poster explains how to measure cell viability easily in 3D cell cultures using the ViaLight™ Plus BioAssay. Cell viability was determined in two different 3D cell culture systems – the RAFT™ 3D Cell Culture System and classical spheroid cultures generated in ultra-low attachment plates.