Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Women’s Immune System Genes Operate Differently from Men’s
A new technology reveals that immune system genes switch on and off differently in women and men, and the source of that variation is not primarily in the DNA.
DNA Damage Seen in Patients Undergoing CT Scanning
Along with the burgeoning use of advanced medical imaging tests over the past decade have come rising public health concerns about possible links between low-dose radiation and cancer.
Yeast Cells Use Signaling Pathway to Modify Their Genomes
Researchers at the Babraham Institute and Cambridge Systems Biology Centre, University of Cambridge have shown that yeast can modify their genomes to take advantage of an excess of calories in the environment and attain optimal growth.
New Material Forges the Way for 'Stem Cell Factories'
Researchers have discovered the first fully synthetic substrate with potential to grow billions of stem cells. The researchcould forge the way for the creation of 'stem cell factories' - the mass production of human embryonic (pluripotent) stem cells.
New Measurements Reveal Differences Between Stem Cells for Treating Retinal Degeneration
By growing two types of stem cells in a “3-D culture” and measuring their ability to produce retinal cells, a team lead by St. Jude Children’s Research Hospital researchers has found one cell type to be better at producing retinal cells.
Researchers Identify Critical Genes Responsible for Brain Tumor Growth
After generating new brain tumor models scientists have identified the role of a family of genes underlying tumor growth in a wide spectrum of high grade brain tumors.
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
A New Path Towards a Universal Flu Vaccine
New research suggests it may be possible to harness a previously unknown mechanism within the immune system to create more effective and efficient vaccines against this ever-mutating virus.
Potential New Class of Cancer Drugs
Scientists have found a way to stop cancer cell growth by targeting the Warburg Effect, a trait of cancer cell metabolism that scientists have been eager to exploit.
Human Trials of Manufactured Blood Within Two Years
The first human trials of lab-produced blood to help create better-matched blood for patients with complex blood conditions has been announced by NHS Blood and Transplant.
Scroll Up
Scroll Down

3D cell culture: a realistic and reproducible collagen-based environment
Bookmark and Share

Tecan Group

In drug discovery and many other areas of research, cell culture using primary cells of human or animal origin is often desirable, or even essential. TAP Biosystems has developed a novel 3D cell culture technique, RAFT (Real Architecture for 3D Tissue), which uses the most abundant matrix protein in the body: type I collagen. 

This technology allows researchers to culture cell types of their choice, including primary cells, in an in vivo like environment that is essentially a hydrogel with tissue-like properties. Uniquely, the RAFT process increases the collagen concentration to physiological levels rapidly and reproducibly, while maintaining high cell viability. 

Tecan and TAP Biosystems have developed an automated process for the preparation of RAFT cell cultures, aiding mixing procedures and enhancing reproducibility while maintaining excellent cell viability and reliability. Implementation on the Freedom EVO system, with its modular automation capabilities, increases experimental versatility and analytical flexibility. 

This application note presents cell viability and proliferation data for primary neonatal human dermal fibroblasts (Life Technologies) obtained over an 11-day culture period after seeding.


Further Information

Related Content

Investigating Protein Targets and Cellular Pathways in Yeasts
Scientists at the Donnelly Centre for Cellular and Biomolecular Research have chosen generations of Tecan microplate readers to monitor the effects of environmental or drug perturbation on molecularly barcoded yeasts.
Monday, October 07, 2013
Tecan and Reinnervate Team up for Automation of 3D Cell Culture
Alvetex Scaffold for the automation of 3D cell culture.
Tuesday, June 26, 2012
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!