Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Urine Excretion From Stem Cell-Derived Kidneys
Researchers report a strategy for enabling urine excretion from kidneys grown from stem cells.
The Black Box at the Beginning of Life
Kyoto University sheds light on the earliest stages of human germ cell development.
Flu Study, on Hold, Yields New Vaccine Technology
Vaccines to protect against an avian influenza pandemic as well as seasonal flu may be mass produced more quickly and efficiently using technology described today by researchers at the University of Wisconsin-Madison.
3D Spheroid Culture Trends
Three dimensional (3D) cell culture has been an area of increasing interest and relevance across a wide breadth of fields for some time.
The Mending Tissue - Cellular Instructions for Tissue Repair
NUS-led collaborative study identifies universal mechanism that explains how tissue shape regulates physiological processes such as wound healing and embryo development.
Most Complete Human Brain Model to Date is a ‘Brain Changer’
Once licensed, model likely to accelerate study of Alzheimer’s, autism, more.
Capturing Cell Growth in 3-D
Spinout’s microfluidics device better models how cancer and other cells interact in the body.
Protein That Turns Moles Into Melanoma Cancer Identified
Moles can turn into cancer, if the genetic factors recently identified by a team of researchers at the University of Pennsylvania were not present in humans.
Scientists Grow Human Serotonin Neurons in Petri Dish
The advance could facilitate the discovery of new antidepressants and drugs for illnesses involving serotonin.
Study Details Powerful Molecular Promoter of Colon Cancers
Findings show how suppression of microRNA family of molecules leads to intestinal tumors.
Scroll Up
Scroll Down

3D cell culture: a realistic and reproducible collagen-based environment
Bookmark and Share

Tecan Group

In drug discovery and many other areas of research, cell culture using primary cells of human or animal origin is often desirable, or even essential. TAP Biosystems has developed a novel 3D cell culture technique, RAFT (Real Architecture for 3D Tissue), which uses the most abundant matrix protein in the body: type I collagen. 

This technology allows researchers to culture cell types of their choice, including primary cells, in an in vivo like environment that is essentially a hydrogel with tissue-like properties. Uniquely, the RAFT process increases the collagen concentration to physiological levels rapidly and reproducibly, while maintaining high cell viability. 

Tecan and TAP Biosystems have developed an automated process for the preparation of RAFT cell cultures, aiding mixing procedures and enhancing reproducibility while maintaining excellent cell viability and reliability. Implementation on the Freedom EVO system, with its modular automation capabilities, increases experimental versatility and analytical flexibility. 

This application note presents cell viability and proliferation data for primary neonatal human dermal fibroblasts (Life Technologies) obtained over an 11-day culture period after seeding.

Further Information

Related Content

Investigating Protein Targets and Cellular Pathways in Yeasts
Scientists at the Donnelly Centre for Cellular and Biomolecular Research have chosen generations of Tecan microplate readers to monitor the effects of environmental or drug perturbation on molecularly barcoded yeasts.
Monday, October 07, 2013
Tecan and Reinnervate Team up for Automation of 3D Cell Culture
Alvetex Scaffold for the automation of 3D cell culture.
Tuesday, June 26, 2012

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos