Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Join | Sign in
Home>Videos>This Video
  Videos

Return

3D Organotypic Liver Cultures and their Application in Predictive Toxicology
SELECTBIO

3D cultivation allows organotypic microtissue formation ensuring cell-cell contacts and contacts to the extracellular matrix. The communication between cells at tight junctions and across extracellular matrix induces and maintains cellular differentiation, functionality and viability. 3D cell cultivation techniques can tremendously improve studies on drug metabolism, drug toxicity especially long-term repeated dose effects. Using a high-throughput 3D cultivation system, we produced 3D organotypic cultures of human hepatic cells using a 96 well plate based hanging drop method. This method allows scaffold-free reorganization of cells under the force of gravity. We show that the production of 3D organotypic cultures of various hepatic cell lines and primary liver cells is possible (Mueller et al., 2011, 2013). The hepatic cells in these 3D cultures were analyzed for viability and functionality. We show that the organotypic cultures maintain high liver-specific function over 3 weeks of culture (Gunness et al., 2013). The effects of several compounds (acetaminophen, aflatoxin B, valproic acid, chlorpromazine, troglitazone and rosiglitazone) were monitored and compared. Currently we are using this system for mechanistic studies. In conclusion, better functionality of 3D systems improves the prediction of toxicity and will have impact in the changed paradigm of drug screening.

Request more information
Company product page


Access to this article and other content is for registered users.

Join the Technology Networks Community

  • Access to the latest scientific news, products and research through Technology Networks
  • Upload and share your posters on ePosters
  • View a library of 1,800+ scientific and medical posters
  • A library of 3,000+ scientific videos on LabTube


Sign In



Forgotten your details? Click Here
If you already have an account with Technology Networks, please use your existing login details. If you do not yet have an account please join here.

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
Study Points to Possible Treatment for Lethal Pediatric Brain Cancer
NIH-funded preclinical study suggests epigenetic drugs may be used to treat leading cause of pediatric brain cancer death.
Simpler Method to Grow Stem Cells
University of Texas at El Paso study shows that stem cells can grow with dead, or fixed, feeder cells.
A New Wrinkle For Cell Culture
Researchers at Brown University have developed an advanced technique for cell culturing that uses sheets of wrinkled graphene to mimic the complex 3-D environment inside the body.
A New Lease on Life for Prostate Tissue
Rutgers Cancer Institute of New Jersey research results in development of organoid prostate models
A New Tool for Understanding ALS: Patients’ Brain Cells
Researchers create a free public library of versatile stem cells from ALS patients.
MIPT Researchers Grow Cardiac Tissue on “Spider Silk” Substrate
Researchers discuss their findings in an article that has recently come out in the journal PLOS ONE.
Scientists Make No Bones About First Study of Osteocyte Cultures on Space Station
Investigation team will analyze the effects of microgravity on this type of bone cell.
Amniotic Stem Cells Demonstrate Healing Potential
Rice University, Texas Children’s Hospital study proves cells promote vasculature in hydrogel therapy.
How the Human Immune System Keeps TB at Bay
Scientists create model of host, bacteria behavior in latent TB infection.
A Single-Cell Breakthrough
UNC School of Medicine scientist Scott Magness and collaborators use their newly developed technology to dissect properties of single stem cells. The advancement will allow researchers to study gastrointestinal disorders and cancers like never before.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters