Corporate Banner
Satellite Banner
Technology Networks Header
Friday, October 31, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > 3D Organotypic Liver Cultures and their Application in Predictive Toxicology
  Videos

Return

3D Organotypic Liver Cultures and their Application in Predictive Toxicology
SELECTBIO

3D cultivation allows organotypic microtissue formation ensuring cell-cell contacts and contacts to the extracellular matrix. The communication between cells at tight junctions and across extracellular matrix induces and maintains cellular differentiation, functionality and viability. 3D cell cultivation techniques can tremendously improve studies on drug metabolism, drug toxicity especially long-term repeated dose effects. Using a high-throughput 3D cultivation system, we produced 3D organotypic cultures of human hepatic cells using a 96 well plate based hanging drop method. This method allows scaffold-free reorganization of cells under the force of gravity. We show that the production of 3D organotypic cultures of various hepatic cell lines and primary liver cells is possible (Mueller et al., 2011, 2013). The hepatic cells in these 3D cultures were analyzed for viability and functionality. We show that the organotypic cultures maintain high liver-specific function over 3 weeks of culture (Gunness et al., 2013). The effects of several compounds (acetaminophen, aflatoxin B, valproic acid, chlorpromazine, troglitazone and rosiglitazone) were monitored and compared. Currently we are using this system for mechanistic studies. In conclusion, better functionality of 3D systems improves the prediction of toxicity and will have impact in the changed paradigm of drug screening.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Eppendorf Award for Young European Investigators 2015: Call for Entries!
Eppendorf Award celebrates 20 years in 2015.
Heart Drug May Help Treat ALS, Mouse Study Shows
Scientists blocked the enzyme in ALS astrocytes using digoxin, a medication used in the treatment of heart failure.
Lab-developed Intestinal Organoids form Mature Human Tissue in Mice
Study produces unprecedented model to study intestinal diseases.
Culture System Replicates Course Of Alzheimer’s Disease
Three-dimensional system should significantly reduce time and costs of drug development.
Researchers Describe New Method for Cell Line Authentication
SNP profiling, an effective method to detect misidentification, contamination, and chromosomal abnormalities in mouse cell lines.
Giant Leap Against Diabetes
Ability to produce embryonic stem cells will allow researchers to push faster toward cure.
Vital New Insight into How we Produce New Brain Cells
Researchers have identified a key mechanism in the birth of new brain cells, with implications for treating brain injury and diseases.
How Fluid Flow Influences Neuron Growth
A University of Texas at Arlington team exploring how neuron growth can be controlled in the lab and, possibly, in the human body has published a new paper in Nature Scientific Reports on how fluid flow could play a significant role.
A New Molecule Allows for an Increase in Stem Cell Transplants
A clinical study using UM171 and a new type of bioreactor developed for stem culture will be initiated in December 2014.
Scientists Discover Neurochemical Imbalance in Schizophrenia
Researchers discovers that neurons from schizophrenia patients secrete higher amounts of three neurotransmitters.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv