Corporate Banner
Satellite Banner
Chromatography
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Malvern Instruments and Novozymes Biopharma Publish Collaborative Study

Published: Monday, October 29, 2012
Last Updated: Monday, October 29, 2012
Bookmark and Share
Recently published article describe collaborative work using light scattering techniques to support formulation development.

In a recently published article, researchers from Novozymes Biopharma (Nottingham, UK) and Malvern Instruments (Malvern, UK) describe collaborative work using light scattering techniques to demonstrate the short- and long-term stability of the novel recombinant human albumins Albucult® and Recombumin®.

These rAlbumins have been developed and optimized to deliver a safe, stable and regulatory-compliant product for therapeutic formulations.

The teams applied the resolving and detection capabilities of size exclusion chromatography light scattering (SEC-LS) and dynamic light scattering (DLS) offered by Malvern systems to monitor the stability of these Novozymes products.

The article ‘Tools for evaluating the stability of human recombinant albumins used in human therapeutics’ can be accessed at www.malvern.com/formulation-development.

Novozymes Biopharma develops and manufactures high quality, animal-free and regulatory-compliant recombinant ingredients and technologies to support the development of innovative pharmaceutical products.

Understanding and manipulating protein behaviour is a central element of the associated research and a core company expertise. The work described here focused on the use of light scattering techniques to test protein stability under a variety of conditions.

Malvern’s Zetasizer APS, Zetasizer Nano ZS and Viscotek TDAmax SEC-LS were used in studies that examined the effects of pH, temperature and concentration on oligomerization and aggregation behaviors. The results can be used to demonstrate short-term stability and predict shelf life.

Both SEC-LS and DLS were found to be useful for the rigorous testing required to understand and confirm protein stability under various conditions.

SEC-LS allows detailed characterization of the type of oligomers or aggregates present in samples of therapeutic proteins. DLS provides a rapid measurement that allows rapid comparison of a number of sample conditions and to indicate the presence of large aggregates and small oligomers or aggregates.

Malvern offers a growing range of systems for protein characterization.

The Zetasizer Nano is one of the most widely applied systems for measuring particle size and molecular size using dynamic light scattering, and protein mobility (zeta potential) by electrophoretic light scattering, while the Zetasizer APS offers robust, simple to operate, dynamic and static light scattering and automates the measurement of samples in industry standard 96- or 384-well plates.

The Viscotek TDAmax is a complete, research grade, temperature controlled, multi-detector SEC system suitable for the molecular weight and molecular size determination of proteins and other macromolecules.

For more about Malvern’s range of systems for protein characterization go to http://www.malvern.com/labeng/industry/protein/proteins.htm.

Novozymes' animal-free, recombinant albumin (rAlbumin) range helps pharmaceutical drug and medical device manufacturers deliver safe and affordable product improvements in a variety of applications.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More than 4,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Malvern Instruments Opens New Multipurpose Facility in Houston
At 22,000 sq. ft. the new building is double the size of Malvern’s previous premises in Houston and houses state-of-the-art laboratories, product development, manufacturing and commercial operations.
Tuesday, March 27, 2012
Malvern Instruments and Postnova Analytics Co-operate
Both companies co-operate to deliver combined solution for characterizing challenging particulate systems.
Friday, February 17, 2012
Malvern Scientists will lead Protein Characterization Workshop at PEGS Summit
Drs Kevin Mattison, Ulf Nobbmann and Jason Sanchez will lead a workshop at the 7th Annual PEGS.
Tuesday, September 13, 2011
Malvern Instruments and Purdue University Present Joint Symposium on Light Scattering Techniques for Nanoparticle Characterization
The symposium will take place on the Purdue Campus at West Lafayette, Indiana.
Thursday, September 08, 2011
Scientific News
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Making Metabolite Identification More Efficient
Metasense combines the industry's most-comprehensive metabolic transformation prediction with efficient analysis of LC/MS analytical measurements to identify, visualize, and report chemical biotransformations.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
Origins of Life Delivered from Space
Researchers have for the first time shown that ribose, a sugar that is one of the building blocks of genetic material in living organisms, may have formed in cometary ices.
Protecting Yourself from Mosquitoes Just Got Easier
New GoClip™ from RESCUE!® offers six days of protection from mosquitoes as well as biting flies and ticks.
Structure of Essential Digestive Enzyme Uncovered
Using a powerful combination of techniques from biophysics to mathematics, researchers have revealed new insights into the mechanism of a liver enzyme that is critical for human health.
Why do Tomatoes Smell "Grassy"?
Researchers identify enzymes that convert the grassy smell of tomatoes into a sweeter scent.
Plasma Biomarkers for Breast Cancer Diagnosis
Plasma lipidomics profiling identified lipid biomarkers in distinguishing early-stage breast cancer from benign lesions.
A Mechanistic Tool for Studying Fungal Pathogens
This study presents an overview of lipid extraction, chromatographic separation and mass spectrometry employed in qualitative and quantitative sphingolipidomics in fungi.
Newborn Screening Test Developed For Rare, Deadly Neurological Disorder
Scientists have developed a new dried blood spot screening test for Niemann-Pick type C, with goal to speed diagnosis and treatment.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!