Cerilliant Introduces 13C-Labeled Internal Standards for Thyroid Hormone Testing by LC-MS/MS

Cerilliant introduces Certified Spiking Solutions® for use in preparation of calibrators and controls of T4-¹³C₆, T3-¹³C₆, and reverse T3-¹³C₆ for monitoring free and total thyroid hormone levels in serum or plasma by LC-MS/MS. Cerilliant’s new thyroid hormone internal standards complement their current offering of native T4, T3, and reverse T3 Certified Reference Materials (CRMs) at concentrations suitable for dilution to the desired concentration in the laboratory’s matrix of choice. 

Disorders of thyroid metabolism affect millions of patients worldwide. Clinical diagnosis and treatment requires testing and monitoring of patient thyroid hormone levels. Currently most thyroid hormone assays are by radioimmunoassay (RIA) which has limited shelf life and lack specificity. In recent years there has been significant push to develop LC-MS/MS methods for quantitation of thyroid hormones with the potential for higher accuracy, better specificity, and the ability to quantitate the different thyroid hormones separately. The choice of internal standard in these LC-MS/MS methods is critical for accurate quantitation of analytes, especially those at low concentrations or where matrix effects are an important consideration such as in thyroid analysis. “Using ¹³C-labeled internal standards for thyroid analysis can eliminate potential issues such as isotopic exchange and minimize separation of later-eluting analytes from a higher substituted deuterium-labeled internal standard.” comments Josh Cooper, PhD and Cerilliant analytical scientist specializing in LC-MS/MS analysis.