Corporate Banner
Satellite Banner
Chromatography
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Radiation Free Analysis of Phosphorylated Proteins

Published: Friday, March 22, 2013
Last Updated: Friday, March 22, 2013
Bookmark and Share
Phos-tag™ acrylamide, agarose, biotin and MS kit available from Alpha Laboratories.

Phosphorylated substances and stabilizing phosphorylated compounds were previously difficult to measure due to chemical instability.

Now they can be readily detected using the Phos-tag™ acrylamide, agarose, biotin and MS kit available exclusively in the UK from Alpha Laboratories.

Protein phosphorylation has a prominent role in signal transduction and the versatile Phos-tag has been adapted to create a number of tools for phosphorylation analysis.

This novel Phos-tag labelling system was developed by the Department of Functional Molecular Science at Hiroshima University and recognizes the phosphate monoester group.

Under neutral physiological pH conditions Phos-tag strongly binds phosphomonoester di-anions enabling it to replace conventional enzyme immunoassay and radioactive isotope methods for capturing substances with a phosphate monoester group.

Post-translational modification by phosphorylation plays a vital role in regulating the function, activity and location of numerous protein targets.

Phos-tag labelled acrylamide is available for electrophoresis applications like SDS-PAGE mobility shift assays and Phos-tag biotin can be used in Western Blot detection in place of an anti-phospho antibody.

Adaptation of the Phos-tag for use in column chromatography, by construction of a column with labelled agarose, enables purification based on phosphate affinity, providing enriched fractions for further phosphoproteomic investigations.

Phos-tag can even provide a radiation free solution for MALDI-TOF/MS analysis.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
‘Smelling’ Prostate Cancer
A research team from the University of Liverpool and the University of the West of England (UWE Bristol) has reached an important milestone towards creating a urine diagnostic test for prostate cancer that could mean that invasive diagnostic procedures that men currently undergo eventually become a thing of the past.
Charting Kidney Cancer Metabolism
Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy.
Portable Kit Can Recover Traces of Chemical Evidence
A chemist at the National Institute of Standards and Technology (NIST) has developed a portable version of his method for recovering trace chemicals such as environmental pollutants and forensic evidence including secret graves and arson fire debris.
Diagnosis of Two Rare Childhood Diseases Improved
For the first time, researchers have developed tests that could improve the diagnosis of two rare childhood diseases known as congenital disorders of glycosylation (CDGs) and metachromatic leukodystrophy, and that could even lead to new treatments for CDGs.
Artemisinin Mechanism Uncovered
New understanding of how artemisinin works could facilitate development of new drugs and therapeutic strategies against malaria.
Cleaning Out the Membrane Each Day
Cell membranes are made up of a lipid bilayer that is constantly changing due to the flux of material in and out of the cell.
AMRI Acquires Whitehouse Laboratories
Strategically extends AMRI's analytical offerings in rapidly expanding area of outsourcing services.
Novel Approach to Understanding Brain Function
Russell Poldrack scanned his brain to create the most detailed map of brain connectivity ever.
New Analysis Technique for Chiral Activity in Molecules
Professor Hyunwoo Kim of the Chemistry Department and his research team have developed a technique that can easily analyze the optical activity of charged compounds by using nuclear magnetic resonance (NMR) spectroscopy.
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!