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Radiation Free Analysis of Phosphorylated Proteins

Published: Friday, March 22, 2013
Last Updated: Friday, March 22, 2013
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Phos-tag™ acrylamide, agarose, biotin and MS kit available from Alpha Laboratories.

Phosphorylated substances and stabilizing phosphorylated compounds were previously difficult to measure due to chemical instability.

Now they can be readily detected using the Phos-tag™ acrylamide, agarose, biotin and MS kit available exclusively in the UK from Alpha Laboratories.

Protein phosphorylation has a prominent role in signal transduction and the versatile Phos-tag has been adapted to create a number of tools for phosphorylation analysis.

This novel Phos-tag labelling system was developed by the Department of Functional Molecular Science at Hiroshima University and recognizes the phosphate monoester group.

Under neutral physiological pH conditions Phos-tag strongly binds phosphomonoester di-anions enabling it to replace conventional enzyme immunoassay and radioactive isotope methods for capturing substances with a phosphate monoester group.

Post-translational modification by phosphorylation plays a vital role in regulating the function, activity and location of numerous protein targets.

Phos-tag labelled acrylamide is available for electrophoresis applications like SDS-PAGE mobility shift assays and Phos-tag biotin can be used in Western Blot detection in place of an anti-phospho antibody.

Adaptation of the Phos-tag for use in column chromatography, by construction of a column with labelled agarose, enables purification based on phosphate affinity, providing enriched fractions for further phosphoproteomic investigations.

Phos-tag can even provide a radiation free solution for MALDI-TOF/MS analysis.

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