In a study using ion chromatography, two of Hamilton’s anion exchange columns demonstrate excellent results. These columns provide reliable results in a variety of buffer compositions, lending themselves to be used in existing instrumentation and methodologies.
Ion chromatography is a reliable method for determining anion concentration in aqueous solutions or environmental samples.
The process analyzes standard inorganic ions typically using a carbonate-bicarbonate buffer as the mobile phase.
The affect of various buffer compositions with the stationary phase is the subject of numerous research discussions.
Test run: Hamilton’s separation columns with three common buffers
A study has now verified to what extent retention time is influenced by buffer composition. To test this, the three most widely used buffer compositions were used with two Hamilton ion chromatography columns.
The columns are part of a new Anion Line that the leading manufacturer of lab technology has developed as a solution for ion chromatography.
The tested columns were Anion Resolution, a high-resolution column with high sample loading capacity, and Anion Fast, developed for fast separation processes.
All Anion Line columns, including the two used in this study, are made of polyetheretherketone (PEEK) hardware. The stationary phase was a PRP-X100 resin that consists of cross-linked polystyrene-divinylbenzene particles functionalized with trimethyl ammonium anion exchange groups.
The Anion Resolution column was a 4.6 x 250 mm with a 7 µm particle size, while the Anion Fast column was 4.6 x 100 mm with a 5 µm particle size.
Test result: excellent results in all buffer compositions (0.1 mM NaSCN was added to all solvents)
In the test run carried out on a Dionex DX 500 ion chromatograph made by Thermo Fisher, both columns delivered excellent results in all three buffer compositions.
The Anion Resolution column (suitable especially for analyzing unknown samples) demonstrated high separation efficiency. The Anion Fast column used above is best for quickly analyzing samples with known components, giving an optimal ratio of good resolution and short analytical run times.
A benefit is that retention time differences between anions can be increased in unknown samples simply by reducing buffer concentration to prevent possible sample signal overlapping in high concentrations.
In fast analysis, on the other hand, the elution of the anions is accelerated by making eluent concentration stronger.
Further test results demonstrate that the quality of the results was independent from the buffer composition and the Anion Resolution and Anion Fast could be easily integrated into existing instrumentation without extra expense.
Chromatographers realize immediately benefit from the high performance of Hamilton columns.