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Thin Layer Chromatographic Analysis of Beta-Lactam Antibiotics

Published: Tuesday, December 10, 2013
Last Updated: Tuesday, December 10, 2013
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TLC is a less expensive and less complicated chromatographic procedure than HPLC and can be successfully used for the preliminary screening of pharmaceutical substances.

Purpose: 
The paper describes some thin layer chromatographic procedures that allow simple and rapid separation and identification of penicillins and cephalosporins from complex mixtures. 

Methods:
Using silicagel GF254 as stationary phase and selecting different mobile phases we succeeded in the separation of the studied beta-lactamins. Our aim was not only to develop a simple, rapid and efficient method for their separation but also the optimization of the analytical conditions. Results: No system will separate all the beta-lactams, but they could be identified when supplementary information is used from color reactions and/or by using additional chromatographic systems.

Conclusions:
The right combination of solvent system and detection method allows the identification of the studied penicillins and cephalosporins and can be successfully used in the preliminary analysis beta-lactam antibiotics. 

The article is published online in Advanced Pharmaceutical Bulletin and is free to access.


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