Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

How Absolute Molar Mass can be Measured Using UHPLC SEC-MALS

Published: Tuesday, April 08, 2014
Last Updated: Tuesday, April 08, 2014
Bookmark and Share
New study highlights benefits of combining light scattering and refractive index detectors designed specifically for UHPLC applications.

New Study Highlights Benefits of Combining Light Scattering and Refractive Index Detectors Designed Specifically for UHPLC Applications.

Wyatt Technology Corporation has announced the availability of a new application note which outlines the combined use of the Wyatt μDAWN™ multi-angle light scattering (MALS) detector and the Optilab® UT-rEX™ refractive index (RI) detector to measure the absolute molecular weight (or molar mass) and size of eluting species in ultra high performance liquid chromatography (UHPLC). The study highlights how these instruments, engineered specifically for use with ultra-high performance size exclusion chromatography (UHP-SEC), overcome the limitations associated with conventional UHP-SEC for the characterization of size and composition of aggregates or fragments in a biomolecular sample.

Conventional SEC relies on reference standards to calibrate column elution time as a function of molar mass. However, these standards often do not accurately represent the conformation of the sample, or the sample may exhibit column interactions that change its elution properties relative to the standard. SEC-MALS with conventional HPLC is commonly utilized to determine molar masses independently of reference standards and elution time. The application note discusses how UHP-SEC-MALS provides improved resolution, higher throughput, less solvent and smaller sample consumption for analysis of precious biological samples in comparison to standard SEC-MALS. Light scattering detectors designed for standard HPLC have generally been incompatible with UHPLC. The powerful combination of the μDAWN and UT-rEX makes UHP-SEC-MALS a reality, resulting in faster and more efficient characterization of biomolecular samples.

The study demonstrates that the new detectors maintain the narrow peak widths typical of UHPLC while providing even more information than standard SEC-MALS thanks to the superb upstream resolution obtained with 300 mm UHPLC columns. The time difference between the two techniques clearly demonstrates how UHP-SEC-MALS results in much faster analyses. The application note also showcases how users can easily transition from existing HPLC methods to UHPLC while maintaining the same accurate, high-quality molecular weight data.

Geoffrey Wyatt, President of Wyatt Technology explains, “We are committed to helping raise standards in molecular analysis, and this new application note demonstrates the absolute quantification of molecules now possible through the use of the μDAWN and the Optilab UT-rEX. Our complete set of detectors enables researchers to enjoy the efficiency of UHPLC without sacrificing the data quality and the ability to measure absolute molar mass of a given protein in solution via MALS. We anticipate that this capability will help researchers achieve unprecedented levels of in-depth analysis for their UHPLC applications.”

With the introduction of the μDAWN, not only is it possible to quantify absolute molecular weight, users also have access to all the in-depth analyses available with the miniDAWN™ TREOS® for UHPLC. This range of instrumentation providing triple detection with UV, MALS, and RI also enables the application of Protein Conjugate Analysis to identify the amount of post-translational modification, associated detergent, drug-conjugate, or other additions to a polypeptide background.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

New Whitepaper from Wyatt on Branching in Synthetic and Natural Polymers
New whitepaper highlights the importance of multi-angle light scattering instrumentation.
Tuesday, February 11, 2014
Wyatt Technology Recognized Among Top 15 Advances

Thursday, December 19, 2013
MALS Detectors Used in Breakthrough Virus Research
Wyatt Technology Corporation have announced that its instruments were used in a recent landmark study that transforms the previous scientific understanding of immunity to viral diseases like the common cold, 'winter vomiting' and gastroenteritis.
Wednesday, January 05, 2011
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
New Analysis Technique for Chiral Activity in Molecules
Professor Hyunwoo Kim of the Chemistry Department and his research team have developed a technique that can easily analyze the optical activity of charged compounds by using nuclear magnetic resonance (NMR) spectroscopy.
Metabolic Profiles Distinguish Early Stage Ovarian Cancer with Unprecedented Accuracy
Studying blood serum compounds of different molecular weights has led scientists to a set of biomarkers that may enable development of a highly accurate screening test for early-stage ovarian cancer.
Genetically Engineering Algae to Kill Cancer Cells
New interdisciplinary research has revealed the frontline role tiny algae could play in the battle against cancer, through the innovative use of nanotechnology.
Acetaldehyde and Formaldehyde Content in Foods
Korean researchers have determined the content of the toxic and carcinogenic aldehydes, acetaldehyde and formaldehyde, in a variety of food groups.
Combining the Power of Mass Spectrometry & Microscopy
A tool that provides world-class microscopy and spatially resolved chemical analysis shows considerable promise for advancing a number of areas of study, including chemical science, pharmaceutical development and disease progression.
Advancing Lithium-Ion Battery Research
The increasing number of components in a lithium-ion battery makes it essential to use a variety of analytical methods to attain complete characterization.
Fruit Fly Pheromone Flags Great Real Estate for Starting a Family
Finding could aid efforts to control mosquito-borne diseases like malaria by manipulating odorants
Education and Expense: The Barriers to Mass Spectrometry in Clinical Laboratories?
Here we examine the perceived barriers to mass spec in clinical laboratories and explore the possible drivers behind the recent shift in uptake of the technology in clinical settings.
Metabolomic Platform Reveals Fundamental Flaw in Common Lab Technology
A new study led by scientists at The Scripps Research Institute (TSRI) shows that a technology used in thousands of laboratories, called gas chromatography mass spectrometry (GC-MS), fundamentally alters the samples it analyzes.

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos