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METHOD DEVELOPMENT FOR THE SIMULTANEOUS DETERMINATION OF METHYLMERCURY AND INORGANIC MERCURY IN SEAFOOD
Sergio Carneado, Ariane V. Zmozinski, Carmen Ibáñez-Palomino, Àngels Sahuquillo, José Fermín López-Sánchez, Márcia M. da Silva

This work reports the method development for the simultaneous determination of methylmercury (MeHg+) and inorganic mercury (iHg) species in seafood. The study focused on the extraction and quantification of MeHg+ by liquid chromatography coupled to on-line UV irradiation and cold vapour atomic fluorescence spectroscopy (LC-UV-HG-AFS), using HCl 4 mol L-1 as extractant agent.

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Application of Central Composite Design (CCD) for the Optimization of Ultrasound-assisted Extraction (UAE) of Phenolics from Corn (Zea mays)
Widiastuti Setyaningsih, 1,2 Erwan Duros, 3 Ceferino Carrera, 4 Miguel Palma, 2 Carmelo G. Barroso 2

A new Ultrasound-assisted Extraction (UAE) of phenolic compounds from corn (Zea mays) has been optimized by means of chemometrics tool. The proposed method was validated and successfully applied to the analysis of phenolics content in several corn cultivars.

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The Longest Way Round is the Shortest Way Home: HTS Assay Development for Complex Multi-domain Protein Kinases
Doris Hafenbrandl, Vanessa Nardese, Maria Cristina Sidoli, Valeria Wanke, Mariantonietta Rubino and Daniele Carettoni

This study strongly supports the possibility to overcome the major bottlenecks in the production of long kinase recombinant forms, in order to perform drug discovery programmes with proteins more closely preserving the structural and functional properties of the native enzymes.

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Characterization of Proteins and Protein Self Association (Oligomerization) with SEC-MALS
Michel Terray, Mark Pothecary

In this poster, a series of proteins and their oligomers were characterized using SEC-MALS with UV and RI detection. The monomer molecular weights are measured and compared with those from column calibration. The molecular weights of their oligomers are also compared with column calibration and the differences explored.

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Amino-Coated Metallofullerene Nanoparticles for Glioblastoma Mutiforme Tumor Detection
Tinghui Li , Susan. Murphy, Kanwarpal Bakshi, Steven LaConte, Zhi Sheng, and Harry Dorn

We report the preparation of a new functionalized trimetallic nitride endohedral metallofullerene, with a cage surface consisting of positively charged amino groups, which is expected to bind more efficiently to negatively charged cell phospholipid bi-layer cellular surfaces and will more readily undergo endocytosis. We now report that this Gd-nanoplatform when subsequently conjugated with an IL-13 peptide, (IL-13-Gd3N@C80(OH)x(NH2)y) exhibits enhanced targeting of U-251 GBM cell lines.

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Significant Increase of Sensitivity on Rapid Influenza Antigen Assays Using Silver Amplification Immunochromatography.
Satoshi KIMURA1), Hideyoshi OHTO2), Hayato YAMAGUCHI1), Seiji FUKUOKA1), Emiko NAKAMA1), Akiko TERADA3), and Yoh UMEDA2)

We have developed a new rapid bed-side assay system for in vitro diagnosis of infectious diseases, using “silver amplification method”, an application of photography-developing technology. The automatic analyzer brings results in fifteen minutes, enhancing sensitivity up to 1000 times compared to the conventional immunochromatography. We applied the technology for diagnosis of hospitalized children. The analyzer was useful for rapid and precise diagnosis of influenza A and B.

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MsCompare: An Untargeted LC & GC/MS Metabolomics Platform for Quality Control, Precise Deconvolution and Data Analysis
Marco Ruijken

A GC/MS or LC/MS workflow for Metabolomics applications includes a number of distinct steps: experimental design, sampling, sample preparation, data acquisition, data processing, deconvolution, identification and data interpretation.

The MsXelerator software includes Quality Control procedures and Analysis of Variance (ANOVA) to properly control each step in this workflow and to obtain reliable results for both quantitation and identification.

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Analysis of 10 systemic pesticide residues in various baby foods using liquid chromatography-tandem mass spectrometry
Jae-Han Shim, Angel Yang, A. M. Abd El-Aty, Jong-Hyouk Park, Ayman Goudah, Md. Musfiqur Rahman, Jung-Ah Do

The method developed was sensitive and selective, and detected the analytes below the MRL established by the European Commission and the Korea Food and Drug Administration. The combination of QuEChERS sample preparation and LC-MS/MS analysis provided acceptable quantitative and qualitative results in baby food.

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Residue Analysis of Etoxazole in Red Pepper using Gas Chromatography: Application to Dissipation Pattern
Sung-Woo Kim, Jin Jang, Md. Musfiqur Rahman, A. M. Abd El-Aty, Ah-Young Ko, Jong-Heui Choi, Jae-Han Shim

The dSPE of QuEChERS was replaced with an SPE cartridge, which provided a very clean chromatogram, although a small recovery percentage was obtained. The method fulfilled all validation criteria and was useful for monitoring etoxazole residues in red pepper. It can also be used and optimized for other fruit and vegetable matrices.

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Scientific News
Investigating the Vape
Expert independent review concludes that e-cigarettes have potential to help smokers quit.
Oceanographers Solve Mystery of Beach Explosion
Scientists at the University of Rhode Island have solved the mystery of what caused an explosion at Salty Brine State Beach in Narrangansett.
New ACE-inhibiting Molecule Found in the Asparagus
Scientists have determined that sulfur-containing compounds in plants can inhibit ACE.
Researchers Pioneer Use of Capsules to Save Materials
Wax capsule delivery systems can simplify a wide range of chemistry transformations.
New Test Could Distinguish Between Bipolar Disorder, Depression
Scientists are developing a test that could tell the difference between bipolar disorder and depression.
Was Shakespeare high?
State-of-the-art forensic technology from South Africa has been used to try and unravel the mystery of what was smoked in tobacco pipes found in the Stratford-upon-Avon garden of British playwright William Shakespeare.
Diabetes Can Cause Immune System Malfunction
Case Western Reserve scientists show how sugar-derived molecules can weaken infection-fighting antimicrobial beta-defensin peptides.
Detecting Hidden Ingredients
Researchers from China have used mass spectrometry to reveal the use of undeclared substances in dietary supplements.
Researchers Find U.S. Breast Milk is Glyphosate Free
Washington State University scientists have found that glyphosate, the main ingredient in the herbicide Roundup, does not accumulate in mother’s breast milk.
Peering into the Vapors
Research suggests that e-cigarettes are much less harmful than previous studies have indicated.
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