Corporate Banner
Satellite Banner
Chromatography
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Researchers Find U.S. Breast Milk is Glyphosate Free
Washington State University scientists have found that glyphosate, the main ingredient in the herbicide Roundup, does not accumulate in mother’s breast milk.
Peering into the Vapors
Research suggests that e-cigarettes are much less harmful than previous studies have indicated.
New Technique for Mining Health-conferring Soy Compounds
A new procedure devised by U.S. Department of Agriculture (USDA) scientists to extract lunasin from soybean seeds could expedite further studies of this peptide for its cancer-fighting potential and other health benefits.
Could a simple saliva test detect Alzheimer's?
Researchers have presented findings suggesting that a simple, non-invasive diagnostic for Alzheimer's could be within reach.
Extracting Ions From A Single Drop of Blood
A new, miniature device that extracts trace ions from just one drop of blood with minimal sample preparation could vastly simplify the analysis of blood samples for certain components.
Improving Snake Antivenom
Scientists are working to determine the effectiveness of antivenom to improve treatment options for people who have been bitten by a poisonous snake.
The Perfect Partnership: Research & Industry; Software & Instrumentation. It really starts to come together at ASMS 2015
Collaboration and knowledge-sharing were evident everywhere: on the bus, in the hallways and in the bars. This article aims to capture this theme and share with you some of the fruits of this coming together of science and industry.
Researchers Use Nanotubes to Better Understand Diseases
Researchers in UC’s Department of Cancer Biology are collaborating with material scientists from the University of Houston to create and use nanotubes to capture and understand the regulation of proteins involved in a variety of diseases.
3D for Top-Down Proteomics: Extra Dimension for More Proteins
Researchers from the University of Wisconsin- Madison have tested the possible applications of adding a "third dimension" to chromatography.
Scientists Come Up with New Technique to Detect Alien Life on Mars
Researchers have provided evidence that combining chromatography/mass spectroscopy with Raman spectroscopy could improve astrobiological studies.
Scroll Up
Scroll Down

Can LC-MS/MS Be Used in Horse Meat Detection?
Bookmark and Share

SCIEX

Most testing methods are based on detection of species-specific DNA in meat, using the polymerase chain reaction (PCR) – which does not detect or identify proteins. This is a concern because DNA can be easily disrupted or removed during standard meat processing and food manufacturing. As a result, horse tissue or other contaminants remain undetected in food samples, despite strong presence of the contaminating proteins. An alternative protein-based method, ELISA (enzyme-linked immunosorbent assay), can be used to complement DNA testing, but this method has limitations, including that it detects only one part of the protein and not multiple protein markers. 

This application note presents an LC-MS/MS-based method that offers a more accurate and reliable approach to meat speciation than PCR or ELISA based techniques or other indirect methods, and also allows for the detection of veterinary drug residues in the same analysis, which is not possible by ELISA or PCR. 

The method was developed using an Eksigent ekspert™ microLC 200 UHPLC system coupled with an AB SCIEX QTRAP® 5500 LC/MS/MS system.

Further Information

Related Content

SCIEX and New Objective Announce Partnership
New nanospray sources to offer maximum sensitivity and robustness for NanoLC-MS.
Tuesday, June 02, 2015
SCIEX and New Objective Partner
Partnership will provide advanced nanospray ionization technologies for next-generation proteomics.
Tuesday, May 26, 2015
Dr. Gyula Vigh wins Arnold O. Beckman Award
SCIEX sponsored award recognizes outstanding contributions to the field of electrodriven separation techniques.
Thursday, May 07, 2015
New Capabilities for Bioanalysis Studies at the International Pharmaceutical Research Center
AB SCIEX Triple Quad™ 6500 LC/MS/MS systems enable new levels of sensitivity for clinical trials of inhaled drugs.
Wednesday, February 11, 2015
AB SCIEX Scientist Wins HUPO 2014 Science and Technology Award
Winner becomes second team member to be recognized for the commercialization of transformative chemistries such as iTRAQ® eagents.
Monday, October 06, 2014
AB SCIEX, Dalton Pharma Services Announce ADC Partnership
Research collaboration aims to develop Antibody-Drug Conjugate analysis capabilities.
Wednesday, August 27, 2014
Barry Karger Receives Arnold O. Beckman Award
Award honors Prof Karger for his outstanding achievements in the field of electrodriven separation techniques.
Monday, April 28, 2014
AB SCIEX to Present ESI-MS Workshop
The workshop will be presented at HPLC 2014.
Tuesday, April 22, 2014
Halal Meat Authenticity Testing Reassures Consumers of No Pig or Horse Contamination
New LC/MS/MS-based meat speciation method allows food testing laboratories to detect pig and horse contamination in diverse food products, with higher accuracy than existing methods.
Tuesday, January 28, 2014
AB SCIEX Brings MASStastic Voyage to Ireland
The tour features a state-of-the-art mobile laboratory and will coincide with a special conference being held by the Irish Separation Science Cluster (ISSC).
Monday, November 11, 2013
AB SCIEX Expands in Singapore
AB SCIEX announce the next major phase of its expansion in Singapore, with the grand opening of a new R&D centre.
Friday, November 01, 2013
AB SCIEX Proteomics Scientist Wins HUPO 2013 Science and Technology Award
Dr. Christie Hunter is being recognized for her contributions to the development and commercialization of a breakthrough approach for targeted proteomics.
Tuesday, September 10, 2013
AB Sciex to Combine Eksigent Chromatography with Capillary Electrophoresis from Beckman
The move brings together Beckman Coulter’s expertise in microscale separation with the expertise in microfluidics of the Eksigent chromatography team of AB Sciex.
Thursday, July 11, 2013
AB SCIEX and LECO Announces Co-marketing Agreement
Bundled solutions leverage the synergy between two technology leaders.
Thursday, July 04, 2013
AB SCIEX Responds to Milk Contamination Concerns with New Method to Detect DCD
Easy-to-use method equips food and beverage testing laboratories around the world for rapid response.
Wednesday, February 13, 2013
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!